Entomological examination of the relationship between ante-mortem and post-mortem amitriptyline concentrations in insects

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dc.contributor.advisorHeyns, Mariseen_ZA
dc.contributor.advisorMole, Calvin Geralden_ZA
dc.contributor.authorSanyanga, Taremekedzwaen_ZA
dc.date.accessioned2016-08-19T13:16:53Z
dc.date.available2016-08-19T13:16:53Z
dc.date.issued2016en_ZA
dc.description.abstractWhen the death of an individual has occurred, the body of the deceased is not always discovered immediately and at times the body may be discovered after a long period of time. The consequence of discovering a body after a long period of time is that the body maybe found severely decomposed or skeletonized. As a result no viable blood, urine or tissue samples may exist that can be collected and utilized in toxicological analyses. Entomotoxicology offers a supplementary method to detect and analyse the presence of drugs post-mortem, especially in cases where viable toxicological specimens such as human tissue cannot be obtained. In South Africa and globally, standardised methodology is required to perform entomological examinations accurately, however due to the large variation of experimental set-up no such standardised methods yet exist. The main aim of the research was to analyse the effect of Amitriptyline on the development and growth rate of forensically important blow flies, and to investigate the potential in using blow fly larvae of the Western Cape, South Africa in forensic entomotoxicological analyses and future implementation. To achieve this blow fly species C. chloropyga and L. sericata were reared on homogenised pig liver containing 1000 mg/kg Amitriptyline until emergence of imago. The duration of time taken by the blow fly larvae species to reach 1 st , 2 nd , 3 rd , pupae and imago growth stages in the presence and absence of Amitriptyline was noted. The presence of drugs in larvae was investigated using high-pressure liquid chromatography coupled with dual mass spectrometry (HPLC/MS-MS). Amitriptyline was detected in all experimental larvae samples and was found to delay pupation by at least 26 hours and imago emergence by at least 72 hours. Amitriptyline however showed no distinct effect on C. chloropyga length and mass but was associated with a smaller length and mass in L. sericata compared to controls. Results indicate that entomotoxicology can be of use for qualitative analysis for the presence of Amitriptyline in forensic cases. Results also highlight the unpredictability of research using biological specimens.en_ZA
dc.identifier.apacitationSanyanga, T. (2016). <i>Entomological examination of the relationship between ante-mortem and post-mortem amitriptyline concentrations in insects</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Forensic Medicine and Toxicology. Retrieved from http://hdl.handle.net/11427/21372en_ZA
dc.identifier.chicagocitationSanyanga, Taremekedzwa. <i>"Entomological examination of the relationship between ante-mortem and post-mortem amitriptyline concentrations in insects."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Forensic Medicine and Toxicology, 2016. http://hdl.handle.net/11427/21372en_ZA
dc.identifier.citationSanyanga, T. 2016. Entomological examination of the relationship between ante-mortem and post-mortem amitriptyline concentrations in insects. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Sanyanga, Taremekedzwa AB - When the death of an individual has occurred, the body of the deceased is not always discovered immediately and at times the body may be discovered after a long period of time. The consequence of discovering a body after a long period of time is that the body maybe found severely decomposed or skeletonized. As a result no viable blood, urine or tissue samples may exist that can be collected and utilized in toxicological analyses. Entomotoxicology offers a supplementary method to detect and analyse the presence of drugs post-mortem, especially in cases where viable toxicological specimens such as human tissue cannot be obtained. In South Africa and globally, standardised methodology is required to perform entomological examinations accurately, however due to the large variation of experimental set-up no such standardised methods yet exist. The main aim of the research was to analyse the effect of Amitriptyline on the development and growth rate of forensically important blow flies, and to investigate the potential in using blow fly larvae of the Western Cape, South Africa in forensic entomotoxicological analyses and future implementation. To achieve this blow fly species C. chloropyga and L. sericata were reared on homogenised pig liver containing 1000 mg/kg Amitriptyline until emergence of imago. The duration of time taken by the blow fly larvae species to reach 1 st , 2 nd , 3 rd , pupae and imago growth stages in the presence and absence of Amitriptyline was noted. The presence of drugs in larvae was investigated using high-pressure liquid chromatography coupled with dual mass spectrometry (HPLC/MS-MS). Amitriptyline was detected in all experimental larvae samples and was found to delay pupation by at least 26 hours and imago emergence by at least 72 hours. Amitriptyline however showed no distinct effect on C. chloropyga length and mass but was associated with a smaller length and mass in L. sericata compared to controls. Results indicate that entomotoxicology can be of use for qualitative analysis for the presence of Amitriptyline in forensic cases. Results also highlight the unpredictability of research using biological specimens. DA - 2016 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2016 T1 - Entomological examination of the relationship between ante-mortem and post-mortem amitriptyline concentrations in insects TI - Entomological examination of the relationship between ante-mortem and post-mortem amitriptyline concentrations in insects UR - http://hdl.handle.net/11427/21372 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21372
dc.identifier.vancouvercitationSanyanga T. Entomological examination of the relationship between ante-mortem and post-mortem amitriptyline concentrations in insects. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Forensic Medicine and Toxicology, 2016 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/21372en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Forensic Medicine and Toxicologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBiomedical Forensic Scienceen_ZA
dc.titleEntomological examination of the relationship between ante-mortem and post-mortem amitriptyline concentrations in insectsen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMPhilen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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