The cyanide degrading nitrilase from Pseudomonas stutzeri AK61 is a two-fold symmetric, 14-subunit spiral

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dc.contributor.authorSewell, B T
dc.contributor.authorBerman, M N
dc.contributor.authorMeyers, P R
dc.contributor.authorJandhyala, D
dc.contributor.authorBenedik, M J
dc.date.accessioned2016-08-04T14:12:52Z
dc.date.available2016-08-04T14:12:52Z
dc.date.issued2003
dc.date.updated2016-08-01T11:22:42Z
dc.description.abstractThe quaternary structure of the cyanide dihydratase from Pseudomonas stutzeri AK61 was determined by negative stain electron microscopy and three-dimensional reconstruction using the single particle technique. The structure is a spiral comprising 14 subunits with 2-fold symmetry. Interactions across the groove cause a decrease in the radius of the spiral at the ends and the resulting steric hindrance prevents the addition of further subunits. Similarity to two members of the nitrilase superfamily, the Nit domain of NitFhit and N-carbamyl-D-amino acid amidohydrolase, enabled the construction of a partial atomic model that could be unambiguously fitted to the stain envelope. The model suggests that interactions involving two significant insertions in the sequence relative to these structures leads to the left-handed spiral assembly.en_ZA
dc.identifierhttp://dx.doi.org/DOI 10.1016/j.str.2003.10.005
dc.identifier.apacitationSewell, B. T., Berman, M. N., Meyers, P. R., Jandhyala, D., & Benedik, M. J. (2003). The cyanide degrading nitrilase from Pseudomonas stutzeri AK61 is a two-fold symmetric, 14-subunit spiral. <i>Structure</i>, http://hdl.handle.net/11427/21125en_ZA
dc.identifier.chicagocitationSewell, B T, M N Berman, P R Meyers, D Jandhyala, and M J Benedik "The cyanide degrading nitrilase from Pseudomonas stutzeri AK61 is a two-fold symmetric, 14-subunit spiral." <i>Structure</i> (2003) http://hdl.handle.net/11427/21125en_ZA
dc.identifier.citationSewell, B. T., Berman, M. N., Meyers, P. R., Jandhyala, D., & Benedik, M. J. (2003). The cyanide degrading nitrilase from Pseudomonas stutzeri AK61 is a two-fold symmetric, 14-subunit spiral. Structure, 11(11), 1413-1422.en_ZA
dc.identifier.issn0969-2126en_ZA
dc.identifier.ris TY - Journal Article AU - Sewell, B T AU - Berman, M N AU - Meyers, P R AU - Jandhyala, D AU - Benedik, M J AB - The quaternary structure of the cyanide dihydratase from Pseudomonas stutzeri AK61 was determined by negative stain electron microscopy and three-dimensional reconstruction using the single particle technique. The structure is a spiral comprising 14 subunits with 2-fold symmetry. Interactions across the groove cause a decrease in the radius of the spiral at the ends and the resulting steric hindrance prevents the addition of further subunits. Similarity to two members of the nitrilase superfamily, the Nit domain of NitFhit and N-carbamyl-D-amino acid amidohydrolase, enabled the construction of a partial atomic model that could be unambiguously fitted to the stain envelope. The model suggests that interactions involving two significant insertions in the sequence relative to these structures leads to the left-handed spiral assembly. DA - 2003 DB - OpenUCT DP - University of Cape Town J1 - Structure LK - https://open.uct.ac.za PB - University of Cape Town PY - 2003 SM - 0969-2126 T1 - The cyanide degrading nitrilase from Pseudomonas stutzeri AK61 is a two-fold symmetric, 14-subunit spiral TI - The cyanide degrading nitrilase from Pseudomonas stutzeri AK61 is a two-fold symmetric, 14-subunit spiral UR - http://hdl.handle.net/11427/21125 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21125
dc.identifier.vancouvercitationSewell BT, Berman MN, Meyers PR, Jandhyala D, Benedik MJ. The cyanide degrading nitrilase from Pseudomonas stutzeri AK61 is a two-fold symmetric, 14-subunit spiral. Structure. 2003; http://hdl.handle.net/11427/21125.en_ZA
dc.languageengen_ZA
dc.publisherElsevieren_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceStructureen_ZA
dc.source.urihttp://www.sciencedirect.com/science/journal/09692126
dc.titleThe cyanide degrading nitrilase from Pseudomonas stutzeri AK61 is a two-fold symmetric, 14-subunit spiralen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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