Brain distribution and release of Cholecystokinin octapeptide
dc.contributor.advisor | Millar, Robert P | en_ZA |
dc.contributor.author | Hudson, Anne Mary | en_ZA |
dc.date.accessioned | 2018-02-12T08:41:03Z | |
dc.date.available | 2018-02-12T08:41:03Z | |
dc.date.issued | 1980 | en_ZA |
dc.description.abstract | In this thesis the in vitro release of immunoreactive CCK₈ (iCCK₈) from rat central nervous system preparations and the regulation of this release have been studied. Rat brain was dissected into the following regions; hypothalamus, cerebral cortex, striatum and thalamus, according to the method of Brownstein, Arimura, Sato et. al. (1975). CCK₈ was found to be distributed throughout these regions (range of 9 - 300 pmoles), with the highest concentration in cortex (300 pmol). In addition, low levels (range 9 - 30 pmoles) of CCK₈ were found in spinal cord, brain stem and cerebellum, in agreement with other workers. Immunohistochemical techniques have demonstrated CCK-like immunoreactivity in nerve cell bodies and fibres throughout brain, particularly in the cortex. Subcellular fractionation of rat brain was used to study the subcellular localisation of CCK. Tissue was homogenised to shear off nerve terminals (synaptosomes) which were purified and used to study the release of the peptide from hypothalamic and extrahypothalamic nerve endings. | en_ZA |
dc.identifier.apacitation | Hudson, A. M. (1980). <i>Brain distribution and release of Cholecystokinin octapeptide</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Chemical Pathology. Retrieved from http://hdl.handle.net/11427/27500 | en_ZA |
dc.identifier.chicagocitation | Hudson, Anne Mary. <i>"Brain distribution and release of Cholecystokinin octapeptide."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Chemical Pathology, 1980. http://hdl.handle.net/11427/27500 | en_ZA |
dc.identifier.citation | Hudson, A. 1980. Brain distribution and release of Cholecystokinin octapeptide. University of Cape Town. | en_ZA |
dc.identifier.ris | TY - Thesis / Dissertation AU - Hudson, Anne Mary AB - In this thesis the in vitro release of immunoreactive CCK₈ (iCCK₈) from rat central nervous system preparations and the regulation of this release have been studied. Rat brain was dissected into the following regions; hypothalamus, cerebral cortex, striatum and thalamus, according to the method of Brownstein, Arimura, Sato et. al. (1975). CCK₈ was found to be distributed throughout these regions (range of 9 - 300 pmoles), with the highest concentration in cortex (300 pmol). In addition, low levels (range 9 - 30 pmoles) of CCK₈ were found in spinal cord, brain stem and cerebellum, in agreement with other workers. Immunohistochemical techniques have demonstrated CCK-like immunoreactivity in nerve cell bodies and fibres throughout brain, particularly in the cortex. Subcellular fractionation of rat brain was used to study the subcellular localisation of CCK. Tissue was homogenised to shear off nerve terminals (synaptosomes) which were purified and used to study the release of the peptide from hypothalamic and extrahypothalamic nerve endings. DA - 1980 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1980 T1 - Brain distribution and release of Cholecystokinin octapeptide TI - Brain distribution and release of Cholecystokinin octapeptide UR - http://hdl.handle.net/11427/27500 ER - | en_ZA |
dc.identifier.uri | http://hdl.handle.net/11427/27500 | |
dc.identifier.vancouvercitation | Hudson AM. Brain distribution and release of Cholecystokinin octapeptide. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Chemical Pathology, 1980 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/27500 | en_ZA |
dc.language.iso | eng | en_ZA |
dc.publisher.department | Division of Chemical Pathology | en_ZA |
dc.publisher.faculty | Faculty of Health Sciences | en_ZA |
dc.publisher.institution | University of Cape Town | |
dc.subject.other | Neurophysiology | en_ZA |
dc.subject.other | Cholecystokinin | en_ZA |
dc.title | Brain distribution and release of Cholecystokinin octapeptide | en_ZA |
dc.type | Master Thesis | |
dc.type.qualificationlevel | Masters | |
dc.type.qualificationname | MSc (Med) | en_ZA |
uct.type.filetype | Text | |
uct.type.filetype | Image | |
uct.type.publication | Research | en_ZA |
uct.type.resource | Thesis | en_ZA |
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