Brain distribution and release of Cholecystokinin octapeptide

dc.contributor.advisorMillar, Robert Pen_ZA
dc.contributor.authorHudson, Anne Maryen_ZA
dc.date.accessioned2018-02-12T08:41:03Z
dc.date.available2018-02-12T08:41:03Z
dc.date.issued1980en_ZA
dc.description.abstractIn this thesis the in vitro release of immunoreactive CCK₈ (iCCK₈) from rat central nervous system preparations and the regulation of this release have been studied. Rat brain was dissected into the following regions; hypothalamus, cerebral cortex, striatum and thalamus, according to the method of Brownstein, Arimura, Sato et. al. (1975). CCK₈ was found to be distributed throughout these regions (range of 9 - 300 pmoles), with the highest concentration in cortex (300 pmol). In addition, low levels (range 9 - 30 pmoles) of CCK₈ were found in spinal cord, brain stem and cerebellum, in agreement with other workers. Immunohistochemical techniques have demonstrated CCK-like immunoreactivity in nerve cell bodies and fibres throughout brain, particularly in the cortex. Subcellular fractionation of rat brain was used to study the subcellular localisation of CCK. Tissue was homogenised to shear off nerve terminals (synaptosomes) which were purified and used to study the release of the peptide from hypothalamic and extrahypothalamic nerve endings.en_ZA
dc.identifier.apacitationHudson, A. M. (1980). <i>Brain distribution and release of Cholecystokinin octapeptide</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Chemical Pathology. Retrieved from http://hdl.handle.net/11427/27500en_ZA
dc.identifier.chicagocitationHudson, Anne Mary. <i>"Brain distribution and release of Cholecystokinin octapeptide."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Chemical Pathology, 1980. http://hdl.handle.net/11427/27500en_ZA
dc.identifier.citationHudson, A. 1980. Brain distribution and release of Cholecystokinin octapeptide. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Hudson, Anne Mary AB - In this thesis the in vitro release of immunoreactive CCK₈ (iCCK₈) from rat central nervous system preparations and the regulation of this release have been studied. Rat brain was dissected into the following regions; hypothalamus, cerebral cortex, striatum and thalamus, according to the method of Brownstein, Arimura, Sato et. al. (1975). CCK₈ was found to be distributed throughout these regions (range of 9 - 300 pmoles), with the highest concentration in cortex (300 pmol). In addition, low levels (range 9 - 30 pmoles) of CCK₈ were found in spinal cord, brain stem and cerebellum, in agreement with other workers. Immunohistochemical techniques have demonstrated CCK-like immunoreactivity in nerve cell bodies and fibres throughout brain, particularly in the cortex. Subcellular fractionation of rat brain was used to study the subcellular localisation of CCK. Tissue was homogenised to shear off nerve terminals (synaptosomes) which were purified and used to study the release of the peptide from hypothalamic and extrahypothalamic nerve endings. DA - 1980 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1980 T1 - Brain distribution and release of Cholecystokinin octapeptide TI - Brain distribution and release of Cholecystokinin octapeptide UR - http://hdl.handle.net/11427/27500 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/27500
dc.identifier.vancouvercitationHudson AM. Brain distribution and release of Cholecystokinin octapeptide. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Chemical Pathology, 1980 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/27500en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Chemical Pathologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherNeurophysiologyen_ZA
dc.subject.otherCholecystokininen_ZA
dc.titleBrain distribution and release of Cholecystokinin octapeptideen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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