Exercise intervention alters HDL subclass distribution and function in obese women
| dc.contributor.author | Woudberg, Nicholas J | |
| dc.contributor.author | Mendham, Amy E | |
| dc.contributor.author | Katz, Arieh A | |
| dc.contributor.author | Goedecke, Julia H | |
| dc.contributor.author | Lecour, Sandrine | |
| dc.date.accessioned | 2018-10-15T14:21:42Z | |
| dc.date.available | 2018-10-15T14:21:42Z | |
| dc.date.issued | 2018-10-10 | |
| dc.date.updated | 2018-10-14T03:22:07Z | |
| dc.description.abstract | Background Obesity is associated with a change in high-density lipoprotein (HDL) function and subclass. Exercise training reduces cardiovascular risk in obese patients. We aimed to explore the effect of an exercise training stimulus on HDL functionality and subclass in obese women. Methods Thirty-two obese black South African women were randomly assigned to exercise (combined aerobic and resistance exercise) or control (no exercise) conditions for 12-weeks. Pre- and post-testing included venous blood sampling for analysis of lipid profile and HDL functionality, by measuring cellular cholesterol efflux capacity, reduction in endothelial vascular cell adhesion molecule (VCAM) expression (anti-inflammatory function), paraoxonase (PON) (antioxidative function) and platelet activating factor acetylhydrolase (PAF-AH) activities (anti-thrombotic function). PON-1 and PAF-AH expression were determined in serum and in isolated HDL using Western blotting. Levels of large, intermediate and small HDL subclasses were measured using the Lipoprint® system. Results Exercise training resulted in a decrease in body mass index (− 1.0 ± 0.5% vs + 1.2 ± 0.6%, p = 0.010), PON activity (− 8.7 ± 2.4% vs + 1.1 ± 3.0%, p = 0.021), PAF-AH serum expression (− 22.1 ± 8.0% vs + 16.9 ± 9.8, p = 0.002), and the distribution of small HDL subclasses (− 10.1 ± 5.4% vs + 15.7 ± 6.6%, p = 0.004) compared to controls. Exercise did not alter HDL cellular cholesterol efflux capacity and anti-inflammatory function. Conclusions These results demonstrate the potential for exercise training to modify HDL subclass distribution and HDL function in obese women. Trial registration Clinical trials number: PACTR201711002789113 . | |
| dc.identifier.apacitation | Woudberg, N. J., Mendham, A. E., Katz, A. A., Goedecke, J. H., & Lecour, S. (2018). Exercise intervention alters HDL subclass distribution and function in obese women. <i>Lipids in Health and Disease</i>, http://hdl.handle.net/11427/28927 | en_ZA |
| dc.identifier.chicagocitation | Woudberg, Nicholas J, Amy E Mendham, Arieh A Katz, Julia H Goedecke, and Sandrine Lecour "Exercise intervention alters HDL subclass distribution and function in obese women." <i>Lipids in Health and Disease</i> (2018) http://hdl.handle.net/11427/28927 | en_ZA |
| dc.identifier.citation | Lipids in Health and Disease. 2018 Oct 10;17(1):232 | |
| dc.identifier.ris | TY - Journal Article AU - Woudberg, Nicholas J AU - Mendham, Amy E AU - Katz, Arieh A AU - Goedecke, Julia H AU - Lecour, Sandrine AB - Background Obesity is associated with a change in high-density lipoprotein (HDL) function and subclass. Exercise training reduces cardiovascular risk in obese patients. We aimed to explore the effect of an exercise training stimulus on HDL functionality and subclass in obese women. Methods Thirty-two obese black South African women were randomly assigned to exercise (combined aerobic and resistance exercise) or control (no exercise) conditions for 12-weeks. Pre- and post-testing included venous blood sampling for analysis of lipid profile and HDL functionality, by measuring cellular cholesterol efflux capacity, reduction in endothelial vascular cell adhesion molecule (VCAM) expression (anti-inflammatory function), paraoxonase (PON) (antioxidative function) and platelet activating factor acetylhydrolase (PAF-AH) activities (anti-thrombotic function). PON-1 and PAF-AH expression were determined in serum and in isolated HDL using Western blotting. Levels of large, intermediate and small HDL subclasses were measured using the Lipoprint® system. Results Exercise training resulted in a decrease in body mass index (− 1.0 ± 0.5% vs + 1.2 ± 0.6%, p = 0.010), PON activity (− 8.7 ± 2.4% vs + 1.1 ± 3.0%, p = 0.021), PAF-AH serum expression (− 22.1 ± 8.0% vs + 16.9 ± 9.8, p = 0.002), and the distribution of small HDL subclasses (− 10.1 ± 5.4% vs + 15.7 ± 6.6%, p = 0.004) compared to controls. Exercise did not alter HDL cellular cholesterol efflux capacity and anti-inflammatory function. Conclusions These results demonstrate the potential for exercise training to modify HDL subclass distribution and HDL function in obese women. Trial registration Clinical trials number: PACTR201711002789113 . DA - 2018-10-10 DB - OpenUCT DP - University of Cape Town J1 - Lipids in Health and Disease LK - https://open.uct.ac.za PB - University of Cape Town PY - 2018 T1 - Exercise intervention alters HDL subclass distribution and function in obese women TI - Exercise intervention alters HDL subclass distribution and function in obese women UR - http://hdl.handle.net/11427/28927 ER - | en_ZA |
| dc.identifier.uri | https://doi.org/10.1186/s12944-018-0879-1 | |
| dc.identifier.uri | http://hdl.handle.net/11427/28927 | |
| dc.identifier.vancouvercitation | Woudberg NJ, Mendham AE, Katz AA, Goedecke JH, Lecour S. Exercise intervention alters HDL subclass distribution and function in obese women. Lipids in Health and Disease. 2018; http://hdl.handle.net/11427/28927. | en_ZA |
| dc.language.iso | en | |
| dc.publisher | BioMed Central | |
| dc.publisher.department | Department of Medicine | en_ZA |
| dc.publisher.faculty | Faculty of Health Sciences | en_ZA |
| dc.publisher.institution | University of Cape Town | |
| dc.rights.holder | The Author(s). | |
| dc.source | Lipids in Health and Disease | |
| dc.source.uri | https://lipidworld.biomedcentral.com/ | |
| dc.subject.other | Obesity | |
| dc.subject.other | Exercise intervention | |
| dc.subject.other | HDL structure | |
| dc.subject.other | Cholesterol efflux | |
| dc.subject.other | HDL subclass | |
| dc.subject.other | Antioxidative | |
| dc.subject.other | Anti-inflammatory | |
| dc.title | Exercise intervention alters HDL subclass distribution and function in obese women | |
| dc.type | Journal Article | |
| uct.type.filetype | Text | |
| uct.type.filetype | Image |