Morphological characterisation of yeast colony growth on solid media using image processing

Journal Article

1998

Permanent link to this Item
http://hdl.handle.net/11427/21282
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Dickason_Morphological_1998.pdf (268.67 KB)
Authors
Dickason, G
Robinson, A
Sadr-kazemi, N
Harrison, S T L
Journal Title

Biotechnology Techniques

Link to Journal
http://link.springer.com/journal/10542
Journal ISSN
Volume Title
Publisher

Springer

Publisher

University of Cape Town

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Abstract
To rapidly determine the effect of environmental factors on yeast growth, a cell counting and colony sizing image analysis method was developed to characterise colony growth on solid media. A digitised microscopic image of the yeast was analysed using the Watershed algorithm for cell number determination and a morphological edge detection for colony size determination. The influence of temperature and physiological stress on yeast growth was then investigated over 12.5 h and data extracted by the image analysis method.
Description
Keywords
Image processing
Watershed algorithm
morphology
growth rate

Reference:

Robinson, A., Sadr-Kazemi, N., Dickason, G., & Harrison, S. T. L. (1998). Morphological characterisation of yeast colony growth on solid media using image processing. Biotechnology techniques, 12(10), 763-767.

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