A cluster of two serine transfer RNA genes from Clostridium acetobutylicum P262

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dc.contributor.advisorWoods, David Ren_ZA
dc.contributor.advisorReid, Sharon Jen_ZA
dc.contributor.authorSealy, Victoria Rosamonden_ZA
dc.date.accessioned2016-08-24T12:54:12Z
dc.date.available2016-08-24T12:54:12Z
dc.date.issued1994en_ZA
dc.descriptionBibliography: pages 105-115.en_ZA
dc.description.abstractA cloning system, using metronidazole as a screening tool and E. coli FI9 as a selection host, was previously established to clone C. acetobutylicum P262 electron transport genes which may play a role in solvent metabolism in this bacterium. In theory, metronidazole would be reduced under anaerobic conditions to a cytotoxic intermediate by C. acetobutylicum P262 electron transport genes or reductive enzymes cloned into a recombinant plasmid. This intermediate would kill the host E. coli FI9. One C. acetobutylicum P262 clone, pMETIOB, was found to render the E. coli strain FI9 sensitive to metronidazole, under anaerobic conditions. A number of subclones of the 2.56kb C. acetobutylicum P262 insert DNA were generated in Bluescript pKS and pSK. A range of exonuclease III deletions of this C. acetobutylicum P262 insert DNA were also generated which were shown to lose the metronidazole sensitivity phenotype on progressive deletion of the insert DNA. In vitro and in vivo protein transcription/translation experiments failed to reveal a protein product that was related to the metronidazole sensitivity phenotype. DNA hybridization confrrmed that the insert DNA of pMETIOB hybridized to C. acetobutylicum P262 chromosomal DNA, but not to E. coli chromosomal DNA. The nucleotide sequence of a 933-bp fragment of the C. acetobutylicum P262 insert DNA was determined.en_ZA
dc.identifier.apacitationSealy, V. R. (1994). <i>A cluster of two serine transfer RNA genes from Clostridium acetobutylicum P262</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/21497en_ZA
dc.identifier.chicagocitationSealy, Victoria Rosamond. <i>"A cluster of two serine transfer RNA genes from Clostridium acetobutylicum P262."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1994. http://hdl.handle.net/11427/21497en_ZA
dc.identifier.citationSealy, V. 1994. A cluster of two serine transfer RNA genes from Clostridium acetobutylicum P262. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Sealy, Victoria Rosamond AB - A cloning system, using metronidazole as a screening tool and E. coli FI9 as a selection host, was previously established to clone C. acetobutylicum P262 electron transport genes which may play a role in solvent metabolism in this bacterium. In theory, metronidazole would be reduced under anaerobic conditions to a cytotoxic intermediate by C. acetobutylicum P262 electron transport genes or reductive enzymes cloned into a recombinant plasmid. This intermediate would kill the host E. coli FI9. One C. acetobutylicum P262 clone, pMETIOB, was found to render the E. coli strain FI9 sensitive to metronidazole, under anaerobic conditions. A number of subclones of the 2.56kb C. acetobutylicum P262 insert DNA were generated in Bluescript pKS and pSK. A range of exonuclease III deletions of this C. acetobutylicum P262 insert DNA were also generated which were shown to lose the metronidazole sensitivity phenotype on progressive deletion of the insert DNA. In vitro and in vivo protein transcription/translation experiments failed to reveal a protein product that was related to the metronidazole sensitivity phenotype. DNA hybridization confrrmed that the insert DNA of pMETIOB hybridized to C. acetobutylicum P262 chromosomal DNA, but not to E. coli chromosomal DNA. The nucleotide sequence of a 933-bp fragment of the C. acetobutylicum P262 insert DNA was determined. DA - 1994 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1994 T1 - A cluster of two serine transfer RNA genes from Clostridium acetobutylicum P262 TI - A cluster of two serine transfer RNA genes from Clostridium acetobutylicum P262 UR - http://hdl.handle.net/11427/21497 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21497
dc.identifier.vancouvercitationSealy VR. A cluster of two serine transfer RNA genes from Clostridium acetobutylicum P262. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1994 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/21497en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMolecular and Cell Biologyen_ZA
dc.titleA cluster of two serine transfer RNA genes from Clostridium acetobutylicum P262en_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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