Magnesium-dependent Association and Folding of Oligonucleosomes Reconstituted with Ubiquitinated H2A

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dc.contributor.authorJason, Laure J M
dc.contributor.authorMoore, Susan C
dc.contributor.authorAusió, Juan
dc.contributor.authorLINDSEY, George
dc.date.accessioned2021-10-08T07:20:47Z
dc.date.available2021-10-08T07:20:47Z
dc.date.issued2001
dc.description.abstractThe MgCl2-induced folding of defined 12-mer nucleosomal arrays, in which ubiquitinated histone H2A (uH2A) replaced H2A, was analyzed by quantitative agarose gel electrophoresis and analytical centrifugation. Both types of analysis showed that uH2A arrays attained a degree of compaction similar to that of control arrays in 2 mM MgCl2. These results indicate that attachment of ubiquitin to H2A has little effect on the ability of nucleosomal arrays to form higher order folded structures in the ionic conditions tested. In contrast, uH2A arrays were found to oligomerize at lower MgCl2 concentrations than control nucleosomal arrays, suggesting that histone ubiquitination may play a role in nucleosomal fiber association.
dc.identifier.apacitationJason, L. J. M., Moore, S. C., Ausió, J., & LINDSEY, G. (2001). Magnesium-dependent Association and Folding of Oligonucleosomes Reconstituted with Ubiquitinated H2A. <i>The Journal of Biological Chemistry</i>, 276(18), 14597 - 14601. http://hdl.handle.net/11427/35004en_ZA
dc.identifier.chicagocitationJason, Laure J M, Susan C Moore, Juan Ausió, and George LINDSEY "Magnesium-dependent Association and Folding of Oligonucleosomes Reconstituted with Ubiquitinated H2A." <i>The Journal of Biological Chemistry</i> 276, 18. (2001): 14597 - 14601. http://hdl.handle.net/11427/35004en_ZA
dc.identifier.citationJason, L.J.M., Moore, S.C., Ausió, J. & LINDSEY, G. 2001. Magnesium-dependent Association and Folding of Oligonucleosomes Reconstituted with Ubiquitinated H2A. <i>The Journal of Biological Chemistry.</i> 276(18):14597 - 14601. http://hdl.handle.net/11427/35004en_ZA
dc.identifier.issn0021-9258
dc.identifier.issn1083-351X
dc.identifier.ris TY - Journal Article AU - Jason, Laure J M AU - Moore, Susan C AU - Ausió, Juan AU - LINDSEY, George AB - The MgCl2-induced folding of defined 12-mer nucleosomal arrays, in which ubiquitinated histone H2A (uH2A) replaced H2A, was analyzed by quantitative agarose gel electrophoresis and analytical centrifugation. Both types of analysis showed that uH2A arrays attained a degree of compaction similar to that of control arrays in 2 mM MgCl2. These results indicate that attachment of ubiquitin to H2A has little effect on the ability of nucleosomal arrays to form higher order folded structures in the ionic conditions tested. In contrast, uH2A arrays were found to oligomerize at lower MgCl2 concentrations than control nucleosomal arrays, suggesting that histone ubiquitination may play a role in nucleosomal fiber association. DA - 2001 DB - OpenUCT DP - University of Cape Town IS - 18 J1 - The Journal of Biological Chemistry LK - https://open.uct.ac.za PY - 2001 SM - 0021-9258 SM - 1083-351X T1 - Magnesium-dependent Association and Folding of Oligonucleosomes Reconstituted with Ubiquitinated H2A TI - Magnesium-dependent Association and Folding of Oligonucleosomes Reconstituted with Ubiquitinated H2A UR - http://hdl.handle.net/11427/35004 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/35004
dc.identifier.vancouvercitationJason LJM, Moore SC, Ausió J, LINDSEY G. Magnesium-dependent Association and Folding of Oligonucleosomes Reconstituted with Ubiquitinated H2A. The Journal of Biological Chemistry. 2001;276(18):14597 - 14601. http://hdl.handle.net/11427/35004.en_ZA
dc.language.isoeng
dc.publisher.departmentDivision of Medical Biochemistry
dc.publisher.facultyFaculty of Health Sciences
dc.sourceThe Journal of Biological Chemistry
dc.source.journalissue18
dc.source.journalvolume276
dc.source.pagination14597 - 14601
dc.source.urihttps://dx.doi.org/10.1074/jbc.M011153200
dc.subject.otherAnimals
dc.subject.otherCattle
dc.subject.otherCentrifugation
dc.subject.otherChickens
dc.subject.otherElectrophoresis, Agar Gel
dc.subject.otherHistones
dc.subject.otherMagnesium Chloride
dc.subject.otherNucleosomes
dc.subject.otherUbiquitins
dc.subject.otherHistones
dc.subject.otherNucleosomes
dc.subject.otherUbiquitins
dc.subject.otherMagnesium Chloride
dc.titleMagnesium-dependent Association and Folding of Oligonucleosomes Reconstituted with Ubiquitinated H2A
dc.typeJournal Article
uct.type.publicationResearch
uct.type.resourceJournal Article
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