Proteomic Analysis of Excretory-Secretory Products of Mesocestoides corti Metacestodes Reveals Potential Suppressors of Dendritic Cell Functions

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dc.contributor.authorVendelova, Emiliaen_ZA
dc.contributor.authorDe Lima, Jeferson Camargoen_ZA
dc.contributor.authorLorenzatto, Karina Rodriguesen_ZA
dc.contributor.authorMonteiro, Karina Marianteen_ZA
dc.contributor.authorMueller, Thomasen_ZA
dc.contributor.authorVeepaschit, Jyotishmanen_ZA
dc.contributor.authorGrimm, Clemensen_ZA
dc.contributor.authorBrehm, Klausen_ZA
dc.contributor.authorHrčková, Gabrielaen_ZA
dc.contributor.authorLutz, Manfred Ben_ZA
dc.contributor.authorFerreira, Henrique Ben_ZA
dc.contributor.authorNono, Justin Komguepen_ZA
dc.date.accessioned2016-10-31T07:38:03Z
dc.date.available2016-10-31T07:38:03Z
dc.date.issued2016en_ZA
dc.description.abstractAuthor Summary: The metacestode larval stages of life-threatening tapeworms grow within the organs of its mammalian hosts, thus causing severe and long-lasting morbidity. Immunosuppression, which mainly depends on factors that are released or leaking from the parasite, plays an important role in both survival and proliferation of the larvae. These parasite-derived molecules are potential targets for developing new anti-parasitic drugs and/or improving the effectiveness of current therapies. Moreover, an optimized use of such factors could help to minimize pathologies resulting from uncontrolled immune responses, like allergies and autoimmune diseases. The authors herein demonstrate that larvae from a parasitic cestode release factors that sufficiently support the suppression of dendritic cells, a set of innate immune cells that recognizes and initiates host immune responses against invading pathogens. Employing modern analytic proteomic tools combined with immunological bioassays, several cestode-derived candidate immunomodulators were identified. This is the first bioassay-guided comprehensive library of candidate immunomodulators from a tissue-dwelling cestode larva. This work validates the unmet value of the Mesocestoides corti system in characterizing the mechanisms of host immunomodulation by metacestodes and reveals the largest database of candidate metacestode-derived immunomodulators until date.en_ZA
dc.identifier.apacitationVendelova, E., De Lima, J. C., Lorenzatto, K. R., Monteiro, K. M., Mueller, T., Veepaschit, J., ... Nono, J. K. (2016). Proteomic Analysis of Excretory-Secretory Products of Mesocestoides corti Metacestodes Reveals Potential Suppressors of Dendritic Cell Functions. <i>PLOS Neglected Tropical Diseases</i>, http://hdl.handle.net/11427/22353en_ZA
dc.identifier.chicagocitationVendelova, Emilia, Jeferson Camargo De Lima, Karina Rodrigues Lorenzatto, Karina Mariante Monteiro, Thomas Mueller, Jyotishman Veepaschit, Clemens Grimm, et al "Proteomic Analysis of Excretory-Secretory Products of Mesocestoides corti Metacestodes Reveals Potential Suppressors of Dendritic Cell Functions." <i>PLOS Neglected Tropical Diseases</i> (2016) http://hdl.handle.net/11427/22353en_ZA
dc.identifier.citationVendelova, E., de Lima, J. C., Lorenzatto, K. R., Monteiro, K. M., Mueller, T., Veepaschit, J., ... & Ferreira, H. B. (2016). Proteomic Analysis of Excretory-Secretory Products of Mesocestoides corti Metacestodes Reveals Potential Suppressors of Dendritic Cell Functions. PLoS Negl Trop Dis, 10(10), e0005061. doi:10.1371/journal.pntd.0005061en_ZA
dc.identifier.ris TY - Journal Article AU - Vendelova, Emilia AU - De Lima, Jeferson Camargo AU - Lorenzatto, Karina Rodrigues AU - Monteiro, Karina Mariante AU - Mueller, Thomas AU - Veepaschit, Jyotishman AU - Grimm, Clemens AU - Brehm, Klaus AU - Hrčková, Gabriela AU - Lutz, Manfred B AU - Ferreira, Henrique B AU - Nono, Justin Komguep AB - Author Summary: The metacestode larval stages of life-threatening tapeworms grow within the organs of its mammalian hosts, thus causing severe and long-lasting morbidity. Immunosuppression, which mainly depends on factors that are released or leaking from the parasite, plays an important role in both survival and proliferation of the larvae. These parasite-derived molecules are potential targets for developing new anti-parasitic drugs and/or improving the effectiveness of current therapies. Moreover, an optimized use of such factors could help to minimize pathologies resulting from uncontrolled immune responses, like allergies and autoimmune diseases. The authors herein demonstrate that larvae from a parasitic cestode release factors that sufficiently support the suppression of dendritic cells, a set of innate immune cells that recognizes and initiates host immune responses against invading pathogens. Employing modern analytic proteomic tools combined with immunological bioassays, several cestode-derived candidate immunomodulators were identified. This is the first bioassay-guided comprehensive library of candidate immunomodulators from a tissue-dwelling cestode larva. This work validates the unmet value of the Mesocestoides corti system in characterizing the mechanisms of host immunomodulation by metacestodes and reveals the largest database of candidate metacestode-derived immunomodulators until date. DA - 2016 DB - OpenUCT DO - 10.1371/journal.pntd.0005061 DP - University of Cape Town J1 - PLOS Neglected Tropical Diseases LK - https://open.uct.ac.za PB - University of Cape Town PY - 2016 T1 - Proteomic Analysis of Excretory-Secretory Products of Mesocestoides corti Metacestodes Reveals Potential Suppressors of Dendritic Cell Functions TI - Proteomic Analysis of Excretory-Secretory Products of Mesocestoides corti Metacestodes Reveals Potential Suppressors of Dendritic Cell Functions UR - http://hdl.handle.net/11427/22353 ER - en_ZA
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pntd.0005061en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/22353
dc.identifier.vancouvercitationVendelova E, De Lima JC, Lorenzatto KR, Monteiro KM, Mueller T, Veepaschit J, et al. Proteomic Analysis of Excretory-Secretory Products of Mesocestoides corti Metacestodes Reveals Potential Suppressors of Dendritic Cell Functions. PLOS Neglected Tropical Diseases. 2016; http://hdl.handle.net/11427/22353.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentInstitute of Infectious Disease and Molecular Medicineen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2016 Vendelova et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLOS Neglected Tropical Diseasesen_ZA
dc.source.urihttp://journals.plos.org/plosntdsen_ZA
dc.subject.otherHost-pathogen interactionsen_ZA
dc.subject.otherLarvaeen_ZA
dc.subject.otherCestodesen_ZA
dc.subject.otherParasitic diseasesen_ZA
dc.subject.otherImmune responseen_ZA
dc.subject.otherGene ontologiesen_ZA
dc.subject.otherElutionen_ZA
dc.subject.otherHost cellsen_ZA
dc.titleProteomic Analysis of Excretory-Secretory Products of Mesocestoides corti Metacestodes Reveals Potential Suppressors of Dendritic Cell Functionsen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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