Demonstration of the Crabtree effect in Phaffia rhodozyma during continuous and fed-batch cultivation
| dc.contributor.author | Reynders, M B | |
| dc.contributor.author | Rawlings, D E | |
| dc.contributor.author | Harrison, S T L | |
| dc.date.accessioned | 2016-08-17T08:42:14Z | |
| dc.date.available | 2016-08-17T08:42:14Z | |
| dc.date.issued | 1997 | |
| dc.date.updated | 2016-08-16T10:32:11Z | |
| dc.description.abstract | By monitoring cell yield and fermentation products during fed-batch and continuous growth, Pfaffia rhodozyma was shown to exhibit the Crabtree effect. In fed-batch culture at feed concentrations of 27 and 55 g glucose/l there was good agreement between the observed biomass formation and that predicted by a mass balance model. At 125 g glucose/l in the feed, biomass formation was less than predicted and fermentation products such as ethanol and acetic acid accumulated in the culture medium. In continuous culture with a feed concentration of 10 g glucose/l, the Crabtree effect became apparent at a dilution rate of 0.1 h -1 . Aerobic fermentation did not occur provided the sugar substrate was maintained at a concentration of less than 0.5 g/l. Although the cell yield coefficient was reduced from 0.5 g/g to 0.16 g/g during aerobic fermentation, the carotenoid content of the cells was unaffected. | en_ZA |
| dc.identifier | http://dx.doi.org/10.1023/A:1018341421122 | |
| dc.identifier.apacitation | Reynders, M. B., Rawlings, D. E., & Harrison, S. T. L. (1997). Demonstration of the Crabtree effect in Phaffia rhodozyma during continuous and fed-batch cultivation. <i>Biotechnology Letters</i>, http://hdl.handle.net/11427/21281 | en_ZA |
| dc.identifier.chicagocitation | Reynders, M B, D E Rawlings, and S T L Harrison "Demonstration of the Crabtree effect in Phaffia rhodozyma during continuous and fed-batch cultivation." <i>Biotechnology Letters</i> (1997) http://hdl.handle.net/11427/21281 | en_ZA |
| dc.identifier.citation | Reynders, M. B., Rawlings, D. E., & Harrison, S. T. L. (1997). Demonstration of the Crabtree effect in Phaffia rhodozyma during continuous and fed-batch cultivation. Biotechnology letters, 19(6), 549-552. | en_ZA |
| dc.identifier.issn | 0141-5492 | en_ZA |
| dc.identifier.ris | TY - Journal Article AU - Reynders, M B AU - Rawlings, D E AU - Harrison, S T L AB - By monitoring cell yield and fermentation products during fed-batch and continuous growth, Pfaffia rhodozyma was shown to exhibit the Crabtree effect. In fed-batch culture at feed concentrations of 27 and 55 g glucose/l there was good agreement between the observed biomass formation and that predicted by a mass balance model. At 125 g glucose/l in the feed, biomass formation was less than predicted and fermentation products such as ethanol and acetic acid accumulated in the culture medium. In continuous culture with a feed concentration of 10 g glucose/l, the Crabtree effect became apparent at a dilution rate of 0.1 h -1 . Aerobic fermentation did not occur provided the sugar substrate was maintained at a concentration of less than 0.5 g/l. Although the cell yield coefficient was reduced from 0.5 g/g to 0.16 g/g during aerobic fermentation, the carotenoid content of the cells was unaffected. DA - 1997 DB - OpenUCT DP - University of Cape Town J1 - Biotechnology Letters LK - https://open.uct.ac.za PB - University of Cape Town PY - 1997 SM - 0141-5492 T1 - Demonstration of the Crabtree effect in Phaffia rhodozyma during continuous and fed-batch cultivation TI - Demonstration of the Crabtree effect in Phaffia rhodozyma during continuous and fed-batch cultivation UR - http://hdl.handle.net/11427/21281 ER - | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/21281 | |
| dc.identifier.vancouvercitation | Reynders MB, Rawlings DE, Harrison STL. Demonstration of the Crabtree effect in Phaffia rhodozyma during continuous and fed-batch cultivation. Biotechnology Letters. 1997; http://hdl.handle.net/11427/21281. | en_ZA |
| dc.language | eng | en_ZA |
| dc.publisher | Springer | en_ZA |
| dc.publisher.institution | University of Cape Town | |
| dc.source | Biotechnology Letters | en_ZA |
| dc.source.uri | http://link.springer.com/journal/10529 | |
| dc.title | Demonstration of the Crabtree effect in Phaffia rhodozyma during continuous and fed-batch cultivation | en_ZA |
| dc.type | Journal Article | en_ZA |
| uct.type.filetype | Text | |
| uct.type.filetype | Image | |
| uct.type.publication | Research | en_ZA |
| uct.type.resource | Article | en_ZA |