Investigation of XvSap promoters from the resurrection plant, Xerophyta viscosa

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dc.contributor.advisorRafudeen, Mohamed Sen_ZA
dc.contributor.authorEllick, Tamaryn Loreanen_ZA
dc.date.accessioned2014-12-28T20:12:15Z
dc.date.available2014-12-28T20:12:15Z
dc.date.issued2012en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractThe XvPSap1 promoter derived from Xerophyta viscosa has been demonstrated to be stress-inducible during dehydration in transgenic Nicotiana tabacum, black Mexican sweetcorn cells and Zea mays. To improve this promoter, for future applications in crop biotechnology, four shortened promoters, XvSap1D, E, F and G were generated by mutagenesis. The generated promoters had circa 50% reduction in size and contained the 5' proximal and 3' distal regions of the XvPsap1 promoter with the internal region removed. The shortened promoters displayed no significant sequence homology to any other known plant promoter, besides XvPSap1. In addition to the shortened promoters, a newly discovered full length XvPSap2 promoter, showing a 56.41% homology with XvPSap 1 was also assessed in this study.en_ZA
dc.identifier.apacitationEllick, T. L. (2012). <i>Investigation of XvSap promoters from the resurrection plant, Xerophyta viscosa</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/10452en_ZA
dc.identifier.chicagocitationEllick, Tamaryn Lorean. <i>"Investigation of XvSap promoters from the resurrection plant, Xerophyta viscosa."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2012. http://hdl.handle.net/11427/10452en_ZA
dc.identifier.citationEllick, T. 2012. Investigation of XvSap promoters from the resurrection plant, Xerophyta viscosa. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Ellick, Tamaryn Lorean AB - The XvPSap1 promoter derived from Xerophyta viscosa has been demonstrated to be stress-inducible during dehydration in transgenic Nicotiana tabacum, black Mexican sweetcorn cells and Zea mays. To improve this promoter, for future applications in crop biotechnology, four shortened promoters, XvSap1D, E, F and G were generated by mutagenesis. The generated promoters had circa 50% reduction in size and contained the 5' proximal and 3' distal regions of the XvPsap1 promoter with the internal region removed. The shortened promoters displayed no significant sequence homology to any other known plant promoter, besides XvPSap1. In addition to the shortened promoters, a newly discovered full length XvPSap2 promoter, showing a 56.41% homology with XvPSap 1 was also assessed in this study. DA - 2012 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 T1 - Investigation of XvSap promoters from the resurrection plant, Xerophyta viscosa TI - Investigation of XvSap promoters from the resurrection plant, Xerophyta viscosa UR - http://hdl.handle.net/11427/10452 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/10452
dc.identifier.vancouvercitationEllick TL. Investigation of XvSap promoters from the resurrection plant, Xerophyta viscosa. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2012 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/10452en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleInvestigation of XvSap promoters from the resurrection plant, Xerophyta viscosaen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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