A proteomic approach for studying insect phylogeny: CAPA peptides of ancient insect taxa (Dictyoptera, Blattoptera) as a test case

Simple item page
dc.contributor.authorRoth, Steffenen_ZA
dc.contributor.authorFromm, Bastianen_ZA
dc.contributor.authorGade, Gerden_ZA
dc.contributor.authorPredel, Reinharden_ZA
dc.date.accessioned2015-11-04T12:01:47Z
dc.date.available2015-11-04T12:01:47Z
dc.date.issued2009en_ZA
dc.description.abstractBACKGROUND: Neuropeptide ligands have to fit exactly into their respective receptors and thus the evolution of the coding regions of their genes is constrained and may be strongly conserved. As such, they may be suitable for the reconstruction of phylogenetic relationships within higher taxa. CAPA peptides of major lineages of cockroaches (Blaberidae, Blattellidae, Blattidae, Polyphagidae, Cryptocercidae) and of the termite Mastotermes darwiniensis were chosen to test the above hypothesis. The phylogenetic relationships within various groups of the taxon Dictyoptera (praying mantids, termites and cockroaches) are still highly disputed. RESULTS: Tandem mass spectrometry of neuropeptides from perisympathetic organs was used to obtain sequence data of CAPA peptides from single specimens; the data were analysed by Maximum Parsimony and Bayesian Interference. The resulting cladograms, taking 61 species into account, show a topology which is in general agreement with recent molecular and morphological phylogenetic analyses, including the recent phylogenetic arrangement placing termites within the cockroaches. When sequence data sets from other neuropeptides, viz. adipokinetic hormones and sulfakinins, were included, the general topology of the cladogram did not change but bootstrap values increased considerably. CONCLUSION: This study represents the first comprehensive survey of neuropeptides of insects for solely phylogenetic purposes and concludes that sequences of short neuropeptides are suitable to complement molecular biological and morphological data for the reconstruction of phylogenetic relationships.en_ZA
dc.identifier.apacitationRoth, S., Fromm, B., Gade, G., & Predel, R. (2009). A proteomic approach for studying insect phylogeny: CAPA peptides of ancient insect taxa (Dictyoptera, Blattoptera) as a test case. <i>BMC Evolutionary Biology</i>, http://hdl.handle.net/11427/14694en_ZA
dc.identifier.chicagocitationRoth, Steffen, Bastian Fromm, Gerd Gade, and Reinhard Predel "A proteomic approach for studying insect phylogeny: CAPA peptides of ancient insect taxa (Dictyoptera, Blattoptera) as a test case." <i>BMC Evolutionary Biology</i> (2009) http://hdl.handle.net/11427/14694en_ZA
dc.identifier.citationRoth, S., Fromm, B., Gäde, G., & Predel, R. (2009). A proteomic approach for studying insect phylogeny: CAPA peptides of ancient insect taxa (Dictyoptera, Blattoptera) as a test case. BMC evolutionary biology, 9(1), 50.en_ZA
dc.identifier.ris TY - Journal Article AU - Roth, Steffen AU - Fromm, Bastian AU - Gade, Gerd AU - Predel, Reinhard AB - BACKGROUND: Neuropeptide ligands have to fit exactly into their respective receptors and thus the evolution of the coding regions of their genes is constrained and may be strongly conserved. As such, they may be suitable for the reconstruction of phylogenetic relationships within higher taxa. CAPA peptides of major lineages of cockroaches (Blaberidae, Blattellidae, Blattidae, Polyphagidae, Cryptocercidae) and of the termite Mastotermes darwiniensis were chosen to test the above hypothesis. The phylogenetic relationships within various groups of the taxon Dictyoptera (praying mantids, termites and cockroaches) are still highly disputed. RESULTS: Tandem mass spectrometry of neuropeptides from perisympathetic organs was used to obtain sequence data of CAPA peptides from single specimens; the data were analysed by Maximum Parsimony and Bayesian Interference. The resulting cladograms, taking 61 species into account, show a topology which is in general agreement with recent molecular and morphological phylogenetic analyses, including the recent phylogenetic arrangement placing termites within the cockroaches. When sequence data sets from other neuropeptides, viz. adipokinetic hormones and sulfakinins, were included, the general topology of the cladogram did not change but bootstrap values increased considerably. CONCLUSION: This study represents the first comprehensive survey of neuropeptides of insects for solely phylogenetic purposes and concludes that sequences of short neuropeptides are suitable to complement molecular biological and morphological data for the reconstruction of phylogenetic relationships. DA - 2009 DB - OpenUCT DO - 10.1186/1471-2148-9-50 DP - University of Cape Town J1 - BMC Evolutionary Biology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2009 T1 - A proteomic approach for studying insect phylogeny: CAPA peptides of ancient insect taxa (Dictyoptera, Blattoptera) as a test case TI - A proteomic approach for studying insect phylogeny: CAPA peptides of ancient insect taxa (Dictyoptera, Blattoptera) as a test case UR - http://hdl.handle.net/11427/14694 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14694
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2148-9-50
dc.identifier.vancouvercitationRoth S, Fromm B, Gade G, Predel R. A proteomic approach for studying insect phylogeny: CAPA peptides of ancient insect taxa (Dictyoptera, Blattoptera) as a test case. BMC Evolutionary Biology. 2009; http://hdl.handle.net/11427/14694.en_ZA
dc.language.isoengen_ZA
dc.publisherBioMed Central Ltden_ZA
dc.publisher.departmentDepartment of Biological Sciencesen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.rights.holder2009 Roth et al; licensee BioMed Central Ltd.en_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_ZA
dc.sourceBMC Evolutionary Biologyen_ZA
dc.source.urihttp://www.biomedcentral.com/bmcevolbiol/en_ZA
dc.subject.otherPerisympathetic Organen_ZA
dc.subject.otherModel Free Analysisen_ZA
dc.subject.otherNeuropeptide Sequenceen_ZA
dc.subject.otherNeurohaemal Organen_ZA
dc.subject.otherBayesian Majority Ruleen_ZA
dc.subject.otherPhylogenetic Analysisen_ZA
dc.titleA proteomic approach for studying insect phylogeny: CAPA peptides of ancient insect taxa (Dictyoptera, Blattoptera) as a test caseen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Roth_Proteomic_approach_insect_phylogeny_2009.pdf
Size:
535.96 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Journal Articles