The inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assay

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dc.contributor.authorHabte, Habtomen_ZA
dc.contributor.authorde Beer, Corenaen_ZA
dc.contributor.authorLotz, Zoeen_ZA
dc.contributor.authorTyler, Marilynen_ZA
dc.contributor.authorSchoeman, Leannen_ZA
dc.contributor.authorKahn, Delawiren_ZA
dc.contributor.authorMall, Anwaren_ZA
dc.date.accessioned2015-10-28T07:09:50Z
dc.date.available2015-10-28T07:09:50Z
dc.date.issued2008en_ZA
dc.description.abstractBACKGROUND:The female reproductive tract is amongst the main routes for Human Immunodeficiency Virus (HIV) transmission. Cervical mucus however is known to protect the female reproductive tract from bacterial invasion and fluid loss and regulates and facilitates sperm transport to the upper reproductive tract. The purpose of this study was to purify and characterize pregnancy plug mucins and determine their anti-HIV-1 activity in an HIV inhibition assay. METHODS: Pregnancy plug mucins were purified by caesium chloride density-gradient ultra-centrifugation and characterized by Western blotting analysis. The anti-HIV-1 activities of the crude pregnancy plug mucus and purified pregnancy plug mucins was determined by incubating them with HIV-1 prior to infection of the human T lymphoblastoid cell line (CEM SS cells). RESULTS: The pregnancy plug mucus had MUC1, MUC2, MUC5AC and MUC5B. The HIV inhibition assay revealed that while the purified pregnancy plug mucins inhibit HIV-1 activity by approximately 97.5%, the crude pregnancy plug mucus failed to inhibit HIV-1 activity. CONCLUSION: Although it is not clear why the crude sample did not inhibit HIV-1 activity, it may be that the amount of mucins in the crude pregnancy plug mucus (which contains water, mucins, lipids, nucleic acids, lactoferrin, lysozyme, immunoglobulins and ions), is insufficient to cause viral inhibition or aggregation.en_ZA
dc.identifier.apacitationHabte, H., de Beer, C., Lotz, Z., Tyler, M., Schoeman, L., Kahn, D., & Mall, A. (2008). The inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assay. <i>Virology Journal</i>, http://hdl.handle.net/11427/14488en_ZA
dc.identifier.chicagocitationHabte, Habtom, Corena de Beer, Zoe Lotz, Marilyn Tyler, Leann Schoeman, Delawir Kahn, and Anwar Mall "The inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assay." <i>Virology Journal</i> (2008) http://hdl.handle.net/11427/14488en_ZA
dc.identifier.citationHabte, H. H., De Beer, C., Lotz, Z. E., Tyler, M. G., Schoeman, L., Kahn, D., & Mall, A. S. (2008). The inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assay. Virol J, 5(59), b16.en_ZA
dc.identifier.ris TY - Journal Article AU - Habte, Habtom AU - de Beer, Corena AU - Lotz, Zoe AU - Tyler, Marilyn AU - Schoeman, Leann AU - Kahn, Delawir AU - Mall, Anwar AB - BACKGROUND:The female reproductive tract is amongst the main routes for Human Immunodeficiency Virus (HIV) transmission. Cervical mucus however is known to protect the female reproductive tract from bacterial invasion and fluid loss and regulates and facilitates sperm transport to the upper reproductive tract. The purpose of this study was to purify and characterize pregnancy plug mucins and determine their anti-HIV-1 activity in an HIV inhibition assay. METHODS: Pregnancy plug mucins were purified by caesium chloride density-gradient ultra-centrifugation and characterized by Western blotting analysis. The anti-HIV-1 activities of the crude pregnancy plug mucus and purified pregnancy plug mucins was determined by incubating them with HIV-1 prior to infection of the human T lymphoblastoid cell line (CEM SS cells). RESULTS: The pregnancy plug mucus had MUC1, MUC2, MUC5AC and MUC5B. The HIV inhibition assay revealed that while the purified pregnancy plug mucins inhibit HIV-1 activity by approximately 97.5%, the crude pregnancy plug mucus failed to inhibit HIV-1 activity. CONCLUSION: Although it is not clear why the crude sample did not inhibit HIV-1 activity, it may be that the amount of mucins in the crude pregnancy plug mucus (which contains water, mucins, lipids, nucleic acids, lactoferrin, lysozyme, immunoglobulins and ions), is insufficient to cause viral inhibition or aggregation. DA - 2008 DB - OpenUCT DO - 10.1186/1743-422X-5-59 DP - University of Cape Town J1 - Virology Journal LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 T1 - The inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assay TI - The inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assay UR - http://hdl.handle.net/11427/14488 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14488
dc.identifier.urihttp://dx.doi.org/10.1186/1743-422X-5-59
dc.identifier.vancouvercitationHabte H, de Beer C, Lotz Z, Tyler M, Schoeman L, Kahn D, et al. The inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assay. Virology Journal. 2008; http://hdl.handle.net/11427/14488.en_ZA
dc.language.isoengen_ZA
dc.publisherBioMed Central Ltden_ZA
dc.publisher.departmentDepartment of Surgeryen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.rights.holder2008 Habte et al; licensee BioMed Central Ltd.en_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_ZA
dc.sourceVirology Journalen_ZA
dc.source.urihttp://www.virologyj.com/en_ZA
dc.subject.otherPregnancy plug mucinsen_ZA
dc.subject.otherHIV-1 activityen_ZA
dc.subject.otherCervical mucusen_ZA
dc.titleThe inhibition of the Human Immunodeficiency Virus type 1 activity by crude and purified human pregnancy plug mucus and mucins in an inhibition assayen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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