Chinks in the armor of the HIV-1 Envelope glycan shield: implications for immune escape from anti-glycan broadly neutralizing antibodies

Simple item page
dc.contributor.authorMoyo, Thandeka
dc.contributor.authorFerreira, Roux-cil
dc.contributor.authorDavids, Reyaaz
dc.contributor.authorSonday, Zarinah
dc.contributor.authorMoore, Penny L
dc.contributor.authorTravers, Simon A
dc.contributor.authorWood, Natasha T
dc.contributor.authorDorfman, Jeffrey Robert
dc.date.accessioned2017-09-14T12:43:07Z
dc.date.available2017-07-15
dc.date.available2017-09-14T12:43:07Z
dc.date.issued2017-01-15
dc.description.abstractGlycans on HIV-1 Envelope serve multiple functions including blocking epitopes from antibodies. We show that removal of glycan 301, a major target of anti-V3/glycan antibodies, has substantially different effects in two viruses. While glycan 301 on Du156.12 blocks epitopes commonly recognized by sera from chronically HIV-1-infected individuals, it does not do so on CAP45.G3, suggesting that removing the 301 glycan has a smaller effect on the integrity of the glycan shield in CAP45.G3. Changes in sensitivity to broadly neutralizing monoclonal antibodies suggest that the interaction between glycan 301 and the CD4 binding site differ substantially between these 2 viruses. Molecular modeling suggests that removal of glycan 301 likely exposes a greater surface area of the V3 and C4 regions in Du156.12. Our data indicate that the contribution of the 301 glycan to resistance to common neutralizing antibodies varies between viruses, allowing for easier selection for its loss in some viruses.en_ZA
dc.identifier.apacitationMoyo, T., Ferreira, R., Davids, R., Sonday, Z., Moore, P. L., Travers, S. A., ... Dorfman, J. R. (2017). Chinks in the armor of the HIV-1 Envelope glycan shield: implications for immune escape from anti-glycan broadly neutralizing antibodies. <i>Virology</i>, http://hdl.handle.net/11427/25211en_ZA
dc.identifier.chicagocitationMoyo, Thandeka, Roux-cil Ferreira, Reyaaz Davids, Zarinah Sonday, Penny L Moore, Simon A Travers, Natasha T Wood, and Jeffrey Robert Dorfman "Chinks in the armor of the HIV-1 Envelope glycan shield: implications for immune escape from anti-glycan broadly neutralizing antibodies." <i>Virology</i> (2017) http://hdl.handle.net/11427/25211en_ZA
dc.identifier.citationMoyo, T., Ferreira, R. C., Davids, R., Sonday, Z., Moore, P. L., Travers, S. A., ... & Dorfman, J. R. (2017). Chinks in the armor of the HIV-1 Envelope glycan shield: Implications for immune escape from anti-glycan broadly neutralizing antibodies. Virology, 501, 12-24.en_ZA
dc.identifier.ris TY - Journal Article AU - Moyo, Thandeka AU - Ferreira, Roux-cil AU - Davids, Reyaaz AU - Sonday, Zarinah AU - Moore, Penny L AU - Travers, Simon A AU - Wood, Natasha T AU - Dorfman, Jeffrey Robert AB - Glycans on HIV-1 Envelope serve multiple functions including blocking epitopes from antibodies. We show that removal of glycan 301, a major target of anti-V3/glycan antibodies, has substantially different effects in two viruses. While glycan 301 on Du156.12 blocks epitopes commonly recognized by sera from chronically HIV-1-infected individuals, it does not do so on CAP45.G3, suggesting that removing the 301 glycan has a smaller effect on the integrity of the glycan shield in CAP45.G3. Changes in sensitivity to broadly neutralizing monoclonal antibodies suggest that the interaction between glycan 301 and the CD4 binding site differ substantially between these 2 viruses. Molecular modeling suggests that removal of glycan 301 likely exposes a greater surface area of the V3 and C4 regions in Du156.12. Our data indicate that the contribution of the 301 glycan to resistance to common neutralizing antibodies varies between viruses, allowing for easier selection for its loss in some viruses. DA - 2017-01-15 DB - OpenUCT DP - University of Cape Town J1 - Virology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2017 T1 - Chinks in the armor of the HIV-1 Envelope glycan shield: implications for immune escape from anti-glycan broadly neutralizing antibodies TI - Chinks in the armor of the HIV-1 Envelope glycan shield: implications for immune escape from anti-glycan broadly neutralizing antibodies UR - http://hdl.handle.net/11427/25211 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/25211
dc.identifier.vancouvercitationMoyo T, Ferreira R, Davids R, Sonday Z, Moore PL, Travers SA, et al. Chinks in the armor of the HIV-1 Envelope glycan shield: implications for immune escape from anti-glycan broadly neutralizing antibodies. Virology. 2017; http://hdl.handle.net/11427/25211.en_ZA
dc.languageengen_ZA
dc.publisherElsevieren_ZA
dc.publisher.departmentDivision of Immunologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceVirologyen_ZA
dc.source.urihttps://www.journals.elsevier.com/virology
dc.subject.otherHIV-1
dc.subject.otherneutralising antibody
dc.titleChinks in the armor of the HIV-1 Envelope glycan shield: implications for immune escape from anti-glycan broadly neutralizing antibodiesen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Moyo 2017 Chinks in glycan shield.pdf
Size:
1.58 MB
Format:
Adobe Portable Document Format
Description:
Publication
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.72 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Journal Articles