Three-dimensional reconstruction of biological macromolecular complexes from in-lens scanning electron micrographs

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dc.contributor.authorWoodward, J D
dc.contributor.authorWepf, R
dc.contributor.authorSewell, B T
dc.date.accessioned2016-09-05T17:59:15Z
dc.date.available2016-09-05T17:59:15Z
dc.date.issued2009
dc.date.updated2016-09-05T13:36:11Z
dc.description.abstractTwo helical samples: F-actin and the bacteriophage T4 tail sheath were reconstructed in three dimensions from contrast enhanced (rotational shadowing and negatively stained) in-lens cryo-field emission scanning electron micrographs, using the iterative real-space helical reconstruction method. The F-actin--and bacteriophage T4 reconstructions compare favourably to an atomic model refined against fibre diffraction data and a cryo-electron microscopy reconstruction, respectively. These results show that single-particle methods, developed for macromolecules imaged in the transmission electron microscope can be applied to cryo-field emission scanning electron micrographs data with appropriate symmetry.en_ZA
dc.identifierhttp://dx.doi.org/10.1111/j.1365-2818.2009.03168.x
dc.identifier.apacitationWoodward, J. D., Wepf, R., & Sewell, B. T. (2009). Three-dimensional reconstruction of biological macromolecular complexes from in-lens scanning electron micrographs. <i>Journal of Microscopy</i>, http://hdl.handle.net/11427/21668en_ZA
dc.identifier.chicagocitationWoodward, J D, R Wepf, and B T Sewell "Three-dimensional reconstruction of biological macromolecular complexes from in-lens scanning electron micrographs." <i>Journal of Microscopy</i> (2009) http://hdl.handle.net/11427/21668en_ZA
dc.identifier.citationWoodward, J. D., Wepf, R., & Sewell, B. T. (2009). Three‐dimensional reconstruction of biological macromolecular complexes from in‐lens scanning electron micrographs. Journal of microscopy, 234(3), 287-292.en_ZA
dc.identifier.issn0022-2720en_ZA
dc.identifier.ris TY - Journal Article AU - Woodward, J D AU - Wepf, R AU - Sewell, B T AB - Two helical samples: F-actin and the bacteriophage T4 tail sheath were reconstructed in three dimensions from contrast enhanced (rotational shadowing and negatively stained) in-lens cryo-field emission scanning electron micrographs, using the iterative real-space helical reconstruction method. The F-actin--and bacteriophage T4 reconstructions compare favourably to an atomic model refined against fibre diffraction data and a cryo-electron microscopy reconstruction, respectively. These results show that single-particle methods, developed for macromolecules imaged in the transmission electron microscope can be applied to cryo-field emission scanning electron micrographs data with appropriate symmetry. DA - 2009 DB - OpenUCT DP - University of Cape Town J1 - Journal of Microscopy LK - https://open.uct.ac.za PB - University of Cape Town PY - 2009 SM - 0022-2720 T1 - Three-dimensional reconstruction of biological macromolecular complexes from in-lens scanning electron micrographs TI - Three-dimensional reconstruction of biological macromolecular complexes from in-lens scanning electron micrographs UR - http://hdl.handle.net/11427/21668 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21668
dc.identifier.vancouvercitationWoodward JD, Wepf R, Sewell BT. Three-dimensional reconstruction of biological macromolecular complexes from in-lens scanning electron micrographs. Journal of Microscopy. 2009; http://hdl.handle.net/11427/21668.en_ZA
dc.languageengen_ZA
dc.publisherWileyen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceJournal of Microscopyen_ZA
dc.source.urihttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2818
dc.subject.otherActin
dc.subject.otherbacteriophage T4 tail
dc.subject.otherhelical reconstruction
dc.subject.otherIHRSR
dc.subject.otherscanning electron microscopy
dc.titleThree-dimensional reconstruction of biological macromolecular complexes from in-lens scanning electron micrographsen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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