An experimental approach to determine the binding mode of yeast linker histone, Hho1p

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dc.contributor.advisorPatterton, Hughen_ZA
dc.contributor.authorSmith, Elizabeth Men_ZA
dc.date.accessioned2014-07-30T17:41:56Z
dc.date.available2014-07-30T17:41:56Z
dc.date.issued2003en_ZA
dc.descriptionBibliography: leaves 108-120.
dc.description.abstractIn this study, we have developed an assay to investigate of the binding mode of yeast linker histone Hholp to nucleosomes, using magnetic bead pull-down assays. To understand the structural and regulatory role of, Hholp, the linker histone in Saccharomyces cerevisiae, and that of higher eukaryotes (Thoma et al., 1979), it is essential to determine the manner in which these histones associate with chromatin. It was previously proposed that Hh01p may bind to two nucleosomes simultaneously, where globular domain one and globular domain two bind to the adjacent nucleosomes.en_ZA
dc.identifier.apacitationSmith, E. M. (2003). <i>An experimental approach to determine the binding mode of yeast linker histone, Hho1p</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4333en_ZA
dc.identifier.chicagocitationSmith, Elizabeth M. <i>"An experimental approach to determine the binding mode of yeast linker histone, Hho1p."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2003. http://hdl.handle.net/11427/4333en_ZA
dc.identifier.citationSmith, E. 2003. An experimental approach to determine the binding mode of yeast linker histone, Hho1p. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Smith, Elizabeth M AB - In this study, we have developed an assay to investigate of the binding mode of yeast linker histone Hholp to nucleosomes, using magnetic bead pull-down assays. To understand the structural and regulatory role of, Hholp, the linker histone in Saccharomyces cerevisiae, and that of higher eukaryotes (Thoma et al., 1979), it is essential to determine the manner in which these histones associate with chromatin. It was previously proposed that Hh01p may bind to two nucleosomes simultaneously, where globular domain one and globular domain two bind to the adjacent nucleosomes. DA - 2003 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2003 T1 - An experimental approach to determine the binding mode of yeast linker histone, Hho1p TI - An experimental approach to determine the binding mode of yeast linker histone, Hho1p UR - http://hdl.handle.net/11427/4333 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/4333
dc.identifier.vancouvercitationSmith EM. An experimental approach to determine the binding mode of yeast linker histone, Hho1p. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2003 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4333en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleAn experimental approach to determine the binding mode of yeast linker histone, Hho1pen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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