Novel methods for the isolation and purification of exoglycosidases

Simple item page
dc.contributor.advisorMerrifield, E Hen_ZA
dc.contributor.authorPannifer, Susanen_ZA
dc.date.accessioned2017-12-13T14:15:58Z
dc.date.available2017-12-13T14:15:58Z
dc.date.issued1989en_ZA
dc.description.abstractA number of exoglycosidases have been prepared from bacterial and plant sources using established methods for the separation of enzymes, in conjunction with certain novel purification systems hitherto not described in the literature for these enzymes. The enzyme, beta-galactosidase from E. coli has been prepared using previously described methods of phase separation and ion-exchange chromatography. As a final step in this purification, the use of a new hydroxyl-rich chromatographic support for the isolation of high-grade enzyme suitable for use in enzyme immunoassays was investigated. Methods have also been studied for the recovery of alpha-mannosidase as a by-product of the procedure used for the extraction of urease from jack bean (Canavalia ensiformis). The inclusion of a novel step involving the use of hydrophobic-interaction chromatography on Phenyl-Sepharose led to excellent recoveries of enzyme suitable for commercial use. Studies on a second glycosidase, beta-N-acetylhexosaminidase, from the same source (jack bean) paved the way for an adaptation of existing purification methods to provide increased yields and an improved quality of enzyme. Since the research unit in which this work was performed is associated with commercial organizations responsible for the preparation and marketing of biologically active products, it is important that the methods of purification described in this thesis are compatible with the requirements for largescale purification.en_ZA
dc.identifier.apacitationPannifer, S. (1989). <i>Novel methods for the isolation and purification of exoglycosidases</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology. Retrieved from http://hdl.handle.net/11427/26600en_ZA
dc.identifier.chicagocitationPannifer, Susan. <i>"Novel methods for the isolation and purification of exoglycosidases."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology, 1989. http://hdl.handle.net/11427/26600en_ZA
dc.identifier.citationPannifer, S. 1989. Novel methods for the isolation and purification of exoglycosidases. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Pannifer, Susan AB - A number of exoglycosidases have been prepared from bacterial and plant sources using established methods for the separation of enzymes, in conjunction with certain novel purification systems hitherto not described in the literature for these enzymes. The enzyme, beta-galactosidase from E. coli has been prepared using previously described methods of phase separation and ion-exchange chromatography. As a final step in this purification, the use of a new hydroxyl-rich chromatographic support for the isolation of high-grade enzyme suitable for use in enzyme immunoassays was investigated. Methods have also been studied for the recovery of alpha-mannosidase as a by-product of the procedure used for the extraction of urease from jack bean (Canavalia ensiformis). The inclusion of a novel step involving the use of hydrophobic-interaction chromatography on Phenyl-Sepharose led to excellent recoveries of enzyme suitable for commercial use. Studies on a second glycosidase, beta-N-acetylhexosaminidase, from the same source (jack bean) paved the way for an adaptation of existing purification methods to provide increased yields and an improved quality of enzyme. Since the research unit in which this work was performed is associated with commercial organizations responsible for the preparation and marketing of biologically active products, it is important that the methods of purification described in this thesis are compatible with the requirements for largescale purification. DA - 1989 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1989 T1 - Novel methods for the isolation and purification of exoglycosidases TI - Novel methods for the isolation and purification of exoglycosidases UR - http://hdl.handle.net/11427/26600 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/26600
dc.identifier.vancouvercitationPannifer S. Novel methods for the isolation and purification of exoglycosidases. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology, 1989 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/26600en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Medical Biochemistry and Structural Biology
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherGlycosidasesen_ZA
dc.subject.otherGlycoside hydrolases - isolation and purificationen_ZA
dc.subject.otherMedical Biochemistryen_ZA
dc.titleNovel methods for the isolation and purification of exoglycosidasesen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
thesis_hsf_1989_pannifer_susan.pdf
Size:
6.07 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Masters