Within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants

Simple item page
dc.contributor.authorUrbino, Cicaen_ZA
dc.contributor.authorGutiérrez, Serafinen_ZA
dc.contributor.authorAntolik, Annaen_ZA
dc.contributor.authorBouazza, Nabilaen_ZA
dc.contributor.authorDoumayrou, Julietteen_ZA
dc.contributor.authorGranier, Martineen_ZA
dc.contributor.authorMartin, Darren Pen_ZA
dc.contributor.authorPeterschmitt, Michelen_ZA
dc.date.accessioned2015-12-28T06:47:21Z
dc.date.available2015-12-28T06:47:21Z
dc.date.issued2013en_ZA
dc.description.abstractTomato yellow leaf curl virus (TYLCV) is a highly damaging begomovirus native to the Middle East. TYLCV has recently spread worldwide, recombining with other begomoviruses. Recent analysis of mixed infections between TYLCV and Tomato leaf curl Comoros begomovirus (ToLCKMV) has shown that, although natural selection preserves certain co-evolved intra-genomic interactions, numerous and diverse recombinants are produced at 120 days post-inoculation (dpi), and recombinant populations from different tomato plants are very divergent. Here, we investigate the population dynamics that lead to such patterns in tomato plants co-infected with TYLCV and ToLCKMV either by agro-inoculation or using the natural whitefly vector Bemisia tabaci . We monitored the frequency of parental and recombinant genotypes independently in 35 plants between 18 and 330 dpi and identified 177 recombinants isolated at different times. Recombinants were detected from 18 dpi and their frequency increased over time to reach about 50% at 150 dpi regardless of the inoculation method. The distribution of breakpoints detected on 96 fully sequenced recombinants was consistent with a continuous generation of new recombinants as well as random and deterministic effects in their maintenance. A severe population bottleneck of around 10 genomes was estimated during early systemic infection-a phenomenon that could account partially for the heterogeneity in recombinant patterns observed among plants. The detection of the same recombinant genome in six of the thirteen plants analysed beyond 30 dpi supported the influence of selection on observed recombination patterns. Moreover, a highly virulent recombinant genotype dominating virus populations within one plant has, apparently, the potential to be maintained in the natural population according to its infectivity, within-host accumulation, and transmission efficiency - all of which were similar or intermediate to those of the parent genotypes. Our results anticipate the outcomes of natural encounters between TYLCV and ToLCKMV.en_ZA
dc.identifier.apacitationUrbino, C., Gutiérrez, S., Antolik, A., Bouazza, N., Doumayrou, J., Granier, M., ... Peterschmitt, M. (2013). Within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants. <i>PLoS One</i>, http://hdl.handle.net/11427/16023en_ZA
dc.identifier.chicagocitationUrbino, Cica, Serafin Gutiérrez, Anna Antolik, Nabila Bouazza, Juliette Doumayrou, Martine Granier, Darren P Martin, and Michel Peterschmitt "Within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants." <i>PLoS One</i> (2013) http://hdl.handle.net/11427/16023en_ZA
dc.identifier.citationUrbino, C., Gutiérrez, S., Antolik, A., Bouazza, N., Doumayrou, J., Granier, M., ... & Peterschmitt, M. (2013). Within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants. PLoS ONE, 8(3). doi:10.1371/journal.pone.0058375en_ZA
dc.identifier.ris TY - Journal Article AU - Urbino, Cica AU - Gutiérrez, Serafin AU - Antolik, Anna AU - Bouazza, Nabila AU - Doumayrou, Juliette AU - Granier, Martine AU - Martin, Darren P AU - Peterschmitt, Michel AB - Tomato yellow leaf curl virus (TYLCV) is a highly damaging begomovirus native to the Middle East. TYLCV has recently spread worldwide, recombining with other begomoviruses. Recent analysis of mixed infections between TYLCV and Tomato leaf curl Comoros begomovirus (ToLCKMV) has shown that, although natural selection preserves certain co-evolved intra-genomic interactions, numerous and diverse recombinants are produced at 120 days post-inoculation (dpi), and recombinant populations from different tomato plants are very divergent. Here, we investigate the population dynamics that lead to such patterns in tomato plants co-infected with TYLCV and ToLCKMV either by agro-inoculation or using the natural whitefly vector Bemisia tabaci . We monitored the frequency of parental and recombinant genotypes independently in 35 plants between 18 and 330 dpi and identified 177 recombinants isolated at different times. Recombinants were detected from 18 dpi and their frequency increased over time to reach about 50% at 150 dpi regardless of the inoculation method. The distribution of breakpoints detected on 96 fully sequenced recombinants was consistent with a continuous generation of new recombinants as well as random and deterministic effects in their maintenance. A severe population bottleneck of around 10 genomes was estimated during early systemic infection-a phenomenon that could account partially for the heterogeneity in recombinant patterns observed among plants. The detection of the same recombinant genome in six of the thirteen plants analysed beyond 30 dpi supported the influence of selection on observed recombination patterns. Moreover, a highly virulent recombinant genotype dominating virus populations within one plant has, apparently, the potential to be maintained in the natural population according to its infectivity, within-host accumulation, and transmission efficiency - all of which were similar or intermediate to those of the parent genotypes. Our results anticipate the outcomes of natural encounters between TYLCV and ToLCKMV. DA - 2013 DB - OpenUCT DO - 10.1371/journal.pone.0058375 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2013 T1 - Within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants TI - Within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants UR - http://hdl.handle.net/11427/16023 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/16023
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0058375
dc.identifier.vancouvercitationUrbino C, Gutiérrez S, Antolik A, Bouazza N, Doumayrou J, Granier M, et al. Within-host dynamics of the emergence of tomato yellow leaf curl virus recombinants. PLoS One. 2013; http://hdl.handle.net/11427/16023.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentInstitute of Infectious Disease and Molecular Medicineen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2013 Urbino et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plosoneen_ZA
dc.subject.otherPlant genomicsen_ZA
dc.subject.otherTomatoesen_ZA
dc.subject.otherLeavesen_ZA
dc.subject.otherCloningen_ZA
dc.subject.otherViral genomicsen_ZA
dc.subject.otherCo-infectionsen_ZA
dc.subject.otherPolymerase chain reactionen_ZA
dc.subject.otherViral vectorsen_ZA
dc.titleWithin-host dynamics of the emergence of tomato yellow leaf curl virus recombinantsen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Urbino_Within_Host_Dynamics_2013.pdf
Size:
1.32 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Journal Articles