A study of crystal matrix extract and urinary prothrombin fragment 1 from a stone-prone and stone-free population.
| dc.contributor.author | Durrbaum, Dawn | |
| dc.contributor.author | Rodgers, Allen L | |
| dc.contributor.author | Sturrock, Edward D | |
| dc.date.accessioned | 2016-08-02T13:26:54Z | |
| dc.date.available | 2016-08-02T13:26:54Z | |
| dc.date.issued | 2001 | |
| dc.date.updated | 2016-08-02T08:02:50Z | |
| dc.description.abstract | South African blacks are immune to urinary calculi whereas whites have an incidence rate similar to that reported in Western societies. Urinary prothrombin fragment 1 (UPTF1) and the crystal matrix extract (CME) from which it is derived have been shown to be potent inhibitors of crystal growth and aggregation in undiluted human urine. The objective of the present study was to isolate CME and UPTF1 from the urines of black and white subjects in order to assess whether either might contribute to the black population's relative stone immunity. CME was isolated from freshly precipitated calcium oxalate (CaOx) crystals and a crystallization study was conducted in synthetic urine. Coulter Counter, 14C-oxalate deposition, and scanning electron microscopy data demonstrated that the extracts from both race groups strongly inhibited CaOx nucleation. The extract derived from the black subjects inhibited nucleation to a greater extent than that from the whites. A phase conversion from COM to COD in the presence of the extracts, in support of the inhibitory effect of CME, was also observed. Purified UPTF1 isolated from both groups' CME was subjected to rigorous biochemical characterization involving matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, protein sequencing by Edman degradation, and amino acid analyses. No differences in molecular weight or amino acid sequence and composition were identified. It is suggested that the more potent inhibitory activity of the extract derived from the black subjects might be related to this group's relative stone immunity. | en_ZA |
| dc.identifier | http://dx.doi.org/10.1007/s002400000163 | |
| dc.identifier.apacitation | Durrbaum, D., Rodgers, A. L., & Sturrock, E. D. (2001). A study of crystal matrix extract and urinary prothrombin fragment 1 from a stone-prone and stone-free population. <i>Urological Research</i>, http://hdl.handle.net/11427/21098 | en_ZA |
| dc.identifier.chicagocitation | Durrbaum, Dawn, Allen L Rodgers, and Edward D Sturrock "A study of crystal matrix extract and urinary prothrombin fragment 1 from a stone-prone and stone-free population." <i>Urological Research</i> (2001) http://hdl.handle.net/11427/21098 | en_ZA |
| dc.identifier.citation | Durrbaum, D., Rodgers, A. L., & Sturrock, E. D. (2001). A study of crystal matrix extract and urinary prothrombin fragment 1 from a stone-prone and stone-free population. Urological research, 29(2), 83-88. | en_ZA |
| dc.identifier.issn | 0300-5623 | en_ZA |
| dc.identifier.ris | TY - Journal Article AU - Durrbaum, Dawn AU - Rodgers, Allen L AU - Sturrock, Edward D AB - South African blacks are immune to urinary calculi whereas whites have an incidence rate similar to that reported in Western societies. Urinary prothrombin fragment 1 (UPTF1) and the crystal matrix extract (CME) from which it is derived have been shown to be potent inhibitors of crystal growth and aggregation in undiluted human urine. The objective of the present study was to isolate CME and UPTF1 from the urines of black and white subjects in order to assess whether either might contribute to the black population's relative stone immunity. CME was isolated from freshly precipitated calcium oxalate (CaOx) crystals and a crystallization study was conducted in synthetic urine. Coulter Counter, 14C-oxalate deposition, and scanning electron microscopy data demonstrated that the extracts from both race groups strongly inhibited CaOx nucleation. The extract derived from the black subjects inhibited nucleation to a greater extent than that from the whites. A phase conversion from COM to COD in the presence of the extracts, in support of the inhibitory effect of CME, was also observed. Purified UPTF1 isolated from both groups' CME was subjected to rigorous biochemical characterization involving matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, protein sequencing by Edman degradation, and amino acid analyses. No differences in molecular weight or amino acid sequence and composition were identified. It is suggested that the more potent inhibitory activity of the extract derived from the black subjects might be related to this group's relative stone immunity. DA - 2001 DB - OpenUCT DP - University of Cape Town J1 - Urological Research LK - https://open.uct.ac.za PB - University of Cape Town PY - 2001 SM - 0300-5623 T1 - A study of crystal matrix extract and urinary prothrombin fragment 1 from a stone-prone and stone-free population TI - A study of crystal matrix extract and urinary prothrombin fragment 1 from a stone-prone and stone-free population UR - http://hdl.handle.net/11427/21098 ER - | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/21098 | |
| dc.identifier.vancouvercitation | Durrbaum D, Rodgers AL, Sturrock ED. A study of crystal matrix extract and urinary prothrombin fragment 1 from a stone-prone and stone-free population. Urological Research. 2001; http://hdl.handle.net/11427/21098. | en_ZA |
| dc.language | eng | en_ZA |
| dc.publisher | Springer | en_ZA |
| dc.publisher.institution | University of Cape Town | |
| dc.source | Urological Research | en_ZA |
| dc.source.uri | http://link.springer.com/journal/240 | |
| dc.subject.other | Crystal matrix extract (CME) | |
| dc.subject.other | Synthetic urine (SU) | |
| dc.subject.other | Calcium oxalate crystals | |
| dc.title | A study of crystal matrix extract and urinary prothrombin fragment 1 from a stone-prone and stone-free population. | en_ZA |
| dc.type | Journal Article | en_ZA |
| uct.type.filetype | Text | |
| uct.type.filetype | Image | |
| uct.type.publication | Research | en_ZA |
| uct.type.resource | Article | en_ZA |