IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection

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dc.contributor.authorHorsnell, William G Cen_ZA
dc.contributor.authorDarby, Matthew Gen_ZA
dc.contributor.authorHoving, Jennifer Cen_ZA
dc.contributor.authorNieuwenhuizen, Natalieen_ZA
dc.contributor.authorMcSorley, Henry Jen_ZA
dc.contributor.authorNdlovu, Hlumanien_ZA
dc.contributor.authorBobat, Saeedaen_ZA
dc.contributor.authorKimberg, Mattien_ZA
dc.contributor.authorKirstein, Franken_ZA
dc.contributor.authorCutler, Anthony Jen_ZA
dc.date.accessioned2016-01-11T06:54:46Z
dc.date.available2016-01-11T06:54:46Z
dc.date.issued2013en_ZA
dc.description.abstractIn this study, B cell function in protective TH2 immunity against N. brasiliensis infection was investigated. Protection against secondary infection depended on IL-4Rα and IL-13; but not IL-4. Protection did not associate with parasite specific antibody responses. Re-infection of B cell-specific IL-4Rα−/− mice resulted in increased worm burdens compared to control mice, despite their equivalent capacity to control primary infection. Impaired protection correlated with reduced lymphocyte IL-13 production and B cell MHC class II and CD86 surface expression. Adoptive transfer of in vivo N. brasiliensis primed IL-4Rα expressing B cells into naïve BALB/c mice, but not IL-4Rα or IL-13 deficient B cells, conferred protection against primary N. brasiliensis infection. This protection required MHC class II compatibility on B cells suggesting cognate interactions by B cells with CD4+ T cells were important to co-ordinate immunity. Furthermore, the rapid nature of these protective effects by B cells suggested non-BCR mediated mechanisms, such as via Toll Like Receptors, was involved, and this was supported by transfer experiments using antigen pulsed Myd88−/− B cells. These data suggest TLR dependent antigen processing by IL-4Rα-responsive B cells producing IL-13 contribute significantly to CD4+ T cell-mediated protective immunity against N. brasiliensis infection.en_ZA
dc.identifier.apacitationHorsnell, W. G. C., Darby, M. G., Hoving, J. C., Nieuwenhuizen, N., McSorley, H. J., Ndlovu, H., ... Cutler, A. J. (2013). IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection. <i>PLoS One</i>, http://hdl.handle.net/11427/16291en_ZA
dc.identifier.chicagocitationHorsnell, William G C, Matthew G Darby, Jennifer C Hoving, Natalie Nieuwenhuizen, Henry J McSorley, Hlumani Ndlovu, Saeeda Bobat, Matti Kimberg, Frank Kirstein, and Anthony J Cutler "IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection." <i>PLoS One</i> (2013) http://hdl.handle.net/11427/16291en_ZA
dc.identifier.citationHorsnell, W. G., Darby, M. G., Hoving, J. C., Nieuwenhuizen, N., McSorley, H. J., Ndlovu, H., ... & Dewals, B. (2013). IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection. PLoS pathogens, 9(10), e1003662. doi:10.1371/journal.ppat.1003662en_ZA
dc.identifier.ris TY - Journal Article AU - Horsnell, William G C AU - Darby, Matthew G AU - Hoving, Jennifer C AU - Nieuwenhuizen, Natalie AU - McSorley, Henry J AU - Ndlovu, Hlumani AU - Bobat, Saeeda AU - Kimberg, Matti AU - Kirstein, Frank AU - Cutler, Anthony J AB - In this study, B cell function in protective TH2 immunity against N. brasiliensis infection was investigated. Protection against secondary infection depended on IL-4Rα and IL-13; but not IL-4. Protection did not associate with parasite specific antibody responses. Re-infection of B cell-specific IL-4Rα−/− mice resulted in increased worm burdens compared to control mice, despite their equivalent capacity to control primary infection. Impaired protection correlated with reduced lymphocyte IL-13 production and B cell MHC class II and CD86 surface expression. Adoptive transfer of in vivo N. brasiliensis primed IL-4Rα expressing B cells into naïve BALB/c mice, but not IL-4Rα or IL-13 deficient B cells, conferred protection against primary N. brasiliensis infection. This protection required MHC class II compatibility on B cells suggesting cognate interactions by B cells with CD4+ T cells were important to co-ordinate immunity. Furthermore, the rapid nature of these protective effects by B cells suggested non-BCR mediated mechanisms, such as via Toll Like Receptors, was involved, and this was supported by transfer experiments using antigen pulsed Myd88−/− B cells. These data suggest TLR dependent antigen processing by IL-4Rα-responsive B cells producing IL-13 contribute significantly to CD4+ T cell-mediated protective immunity against N. brasiliensis infection. DA - 2013 DB - OpenUCT DO - 10.1371/journal.ppat.1003662 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2013 T1 - IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection TI - IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection UR - http://hdl.handle.net/11427/16291 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/16291
dc.identifier.urihttp://dx.doi.org/10.1371/journal.ppat.1003662
dc.identifier.vancouvercitationHorsnell WGC, Darby MG, Hoving JC, Nieuwenhuizen N, McSorley HJ, Ndlovu H, et al. IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection. PLoS One. 2013; http://hdl.handle.net/11427/16291.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentDivision of Immunologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2013 Horsnell et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plospathogensen_ZA
dc.subject.otherB cellsen_ZA
dc.subject.otherCell-mediated immunityen_ZA
dc.subject.otherT cellsen_ZA
dc.subject.otherNematode infectionsen_ZA
dc.subject.otherLymph nodesen_ZA
dc.subject.otherLarvaeen_ZA
dc.subject.otherHelminth infectionsen_ZA
dc.subject.otherIntestinal parasitesen_ZA
dc.titleIL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infectionen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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