Characterization of transcripts expressed from the Meis2 locus in the Natal Long-fingered bat (Miniopterus natalensis)

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dc.contributor.advisorIlling, Nicolaen_ZA
dc.contributor.advisorJacobs, David Sen_ZA
dc.contributor.authorCurry, Lyleen_ZA
dc.date.accessioned2014-11-05T03:47:00Z
dc.date.available2014-11-05T03:47:00Z
dc.date.issued2014en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractThe Myeloid ecotropic insertion site 2 (Meis2) gene is an important transcriptional regulator involved in the pattering of the limb during vertebrate development. In the forelimbs (wings) of bats the expression Meis2 was shown to be differentially expressed when compared to mice forelimbs. Meis2 was found to be present in the interdigital webbing of the autopod (hand) of the developing bat forelimb. In both the mouse and the bat, two separate transcripts were discovered to be expressed from the 3’and 5’ region of the Meis2 locus. The 3’ transcripts corresponded to annotated Meis2 mRNA transcripts, the 5’ transcript corresponded to an mRNA transcript that did not code for any known protein. The 5’ transcript was thought to be a long non-coding RNA and was termed lncMeis2. In this study Random Amplification of cDNA Ends (RACE) analysis was used to identify the RNA transcripts expressed from the Meis2 locus and to verify the presence of lncMeis2. RACE was performed on the heads and forelimbs of the Natal Long-fingered bat (Miniopterus natalensis) and the mouse (Mus musculus) and the products sequenced and aligned to the mouse genome for verification.en_ZA
dc.identifier.apacitationCurry, L. (2014). <i>Characterization of transcripts expressed from the Meis2 locus in the Natal Long-fingered bat (Miniopterus natalensis)</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/9112en_ZA
dc.identifier.chicagocitationCurry, Lyle. <i>"Characterization of transcripts expressed from the Meis2 locus in the Natal Long-fingered bat (Miniopterus natalensis)."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2014. http://hdl.handle.net/11427/9112en_ZA
dc.identifier.citationCurry, L. 2014. Characterization of transcripts expressed from the Meis2 locus in the Natal Long-fingered bat (Miniopterus natalensis). University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Curry, Lyle AB - The Myeloid ecotropic insertion site 2 (Meis2) gene is an important transcriptional regulator involved in the pattering of the limb during vertebrate development. In the forelimbs (wings) of bats the expression Meis2 was shown to be differentially expressed when compared to mice forelimbs. Meis2 was found to be present in the interdigital webbing of the autopod (hand) of the developing bat forelimb. In both the mouse and the bat, two separate transcripts were discovered to be expressed from the 3’and 5’ region of the Meis2 locus. The 3’ transcripts corresponded to annotated Meis2 mRNA transcripts, the 5’ transcript corresponded to an mRNA transcript that did not code for any known protein. The 5’ transcript was thought to be a long non-coding RNA and was termed lncMeis2. In this study Random Amplification of cDNA Ends (RACE) analysis was used to identify the RNA transcripts expressed from the Meis2 locus and to verify the presence of lncMeis2. RACE was performed on the heads and forelimbs of the Natal Long-fingered bat (Miniopterus natalensis) and the mouse (Mus musculus) and the products sequenced and aligned to the mouse genome for verification. DA - 2014 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2014 T1 - Characterization of transcripts expressed from the Meis2 locus in the Natal Long-fingered bat (Miniopterus natalensis) TI - Characterization of transcripts expressed from the Meis2 locus in the Natal Long-fingered bat (Miniopterus natalensis) UR - http://hdl.handle.net/11427/9112 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/9112
dc.identifier.vancouvercitationCurry L. Characterization of transcripts expressed from the Meis2 locus in the Natal Long-fingered bat (Miniopterus natalensis). [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2014 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/9112en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.titleCharacterization of transcripts expressed from the Meis2 locus in the Natal Long-fingered bat (Miniopterus natalensis)en_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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