Effect of standard tuberculosis treatment on plasma cytokine levels in patients with active pulmonary tuberculosis

dc.contributor.authorRiou, Catherineen_ZA
dc.contributor.authorPeixoto, Blas Perezen_ZA
dc.contributor.authorRoberts, Lindien_ZA
dc.contributor.authorRonacher, Katharinaen_ZA
dc.contributor.authorWalzl, Gerharden_ZA
dc.contributor.authorManca, Claudiaen_ZA
dc.contributor.authorRustomjee, Roxanaen_ZA
dc.contributor.authorMthiyane, Thulien_ZA
dc.contributor.authorFallows, Dorothyen_ZA
dc.contributor.authorGray, Clive Men_ZA
dc.date.accessioned2015-11-23T12:35:25Z
dc.date.available2015-11-23T12:35:25Z
dc.date.issued2012en_ZA
dc.description.abstractBACKGROUND: Sputum Mycobacterium tuberculosis (Mtb) culture is commonly used to assess response to antibiotic treatment in individuals with pulmonary tuberculosis (TB). Such techniques are constrained by the slow growth rate of Mtb, and more sensitive methods to monitor Mtb clearance are needed. The goal of this study was to evaluate changes in plasma cytokines in patients undergoing treatment for TB as a means of identifying candidate host markers associated with microbiologic response to therapy. METHODS: Twenty-four plasma cytokines/chemokines were measured in 42 individuals diagnosed with active pulmonary TB, 52% were HIV co-infected. Individuals, undergoing a 26-week standard TB treatment, were followed longitudinally over 18 months and measurements were associated with HIV status and rates of sputum culture conversion. RESULTS: Plasma concentrations of interferon-inducible protein-10 (IP-10) and vascular endothelial growth factor (VEGF) were significantly reduced upon TB treatment, regardless of HIV status. By the end of treatment, IP-10 concentrations were significantly lower in HIV negative individuals when compared to HIV-positive individuals (p = 0.02). Moreover, in HIV negative patients, plasma VEGF concentrations, measured as early as 2-weeks post TB treatment initiation, positively correlated with the time of sputum conversion (p = 0.0017). No significant changes were observed in other studied immune mediators. CONCLUSIONS: These data suggest that VEGF plasma concentration, measured during early TB treatment, could represent a surrogate marker to monitor sputum culture conversion in HIV uninfected individuals.en_ZA
dc.identifier.apacitationRiou, C., Peixoto, B. P., Roberts, L., Ronacher, K., Walzl, G., Manca, C., ... Gray, C. M. (2012). Effect of standard tuberculosis treatment on plasma cytokine levels in patients with active pulmonary tuberculosis. <i>PLoS One</i>, http://hdl.handle.net/11427/15329en_ZA
dc.identifier.chicagocitationRiou, Catherine, Blas Perez Peixoto, Lindi Roberts, Katharina Ronacher, Gerhard Walzl, Claudia Manca, Roxana Rustomjee, Thuli Mthiyane, Dorothy Fallows, and Clive M Gray "Effect of standard tuberculosis treatment on plasma cytokine levels in patients with active pulmonary tuberculosis." <i>PLoS One</i> (2012) http://hdl.handle.net/11427/15329en_ZA
dc.identifier.citationRiou, C., Perez Peixoto, B., Roberts, L., Ronacher, K., Walzl, G., Manca, C., ... & Kaplan, G. (2012). Effect of standard tuberculosis treatment on plasma cytokine levels in patients with active pulmonary tuberculosis. PloS one, 7(5), e36886. doi:10.1371/journal.pone.0036886en_ZA
dc.identifier.ris TY - Journal Article AU - Riou, Catherine AU - Peixoto, Blas Perez AU - Roberts, Lindi AU - Ronacher, Katharina AU - Walzl, Gerhard AU - Manca, Claudia AU - Rustomjee, Roxana AU - Mthiyane, Thuli AU - Fallows, Dorothy AU - Gray, Clive M AB - BACKGROUND: Sputum Mycobacterium tuberculosis (Mtb) culture is commonly used to assess response to antibiotic treatment in individuals with pulmonary tuberculosis (TB). Such techniques are constrained by the slow growth rate of Mtb, and more sensitive methods to monitor Mtb clearance are needed. The goal of this study was to evaluate changes in plasma cytokines in patients undergoing treatment for TB as a means of identifying candidate host markers associated with microbiologic response to therapy. METHODS: Twenty-four plasma cytokines/chemokines were measured in 42 individuals diagnosed with active pulmonary TB, 52% were HIV co-infected. Individuals, undergoing a 26-week standard TB treatment, were followed longitudinally over 18 months and measurements were associated with HIV status and rates of sputum culture conversion. RESULTS: Plasma concentrations of interferon-inducible protein-10 (IP-10) and vascular endothelial growth factor (VEGF) were significantly reduced upon TB treatment, regardless of HIV status. By the end of treatment, IP-10 concentrations were significantly lower in HIV negative individuals when compared to HIV-positive individuals (p = 0.02). Moreover, in HIV negative patients, plasma VEGF concentrations, measured as early as 2-weeks post TB treatment initiation, positively correlated with the time of sputum conversion (p = 0.0017). No significant changes were observed in other studied immune mediators. CONCLUSIONS: These data suggest that VEGF plasma concentration, measured during early TB treatment, could represent a surrogate marker to monitor sputum culture conversion in HIV uninfected individuals. DA - 2012 DB - OpenUCT DO - 10.1371/journal.pone.0036886 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 T1 - Effect of standard tuberculosis treatment on plasma cytokine levels in patients with active pulmonary tuberculosis TI - Effect of standard tuberculosis treatment on plasma cytokine levels in patients with active pulmonary tuberculosis UR - http://hdl.handle.net/11427/15329 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/15329
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0036886
dc.identifier.vancouvercitationRiou C, Peixoto BP, Roberts L, Ronacher K, Walzl G, Manca C, et al. Effect of standard tuberculosis treatment on plasma cytokine levels in patients with active pulmonary tuberculosis. PLoS One. 2012; http://hdl.handle.net/11427/15329.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentDivision of Immunologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2012 Riou et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plosoneen_ZA
dc.subject.otherCytokinesen_ZA
dc.subject.otherSputumen_ZA
dc.subject.otherTuberculosisen_ZA
dc.subject.otherHIVen_ZA
dc.subject.otherMycobacterium tuberculosisen_ZA
dc.subject.otherCytokine therapyen_ZA
dc.subject.otherPrincipal component analysisen_ZA
dc.subject.otherHIV infectionsen_ZA
dc.titleEffect of standard tuberculosis treatment on plasma cytokine levels in patients with active pulmonary tuberculosisen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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