Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco

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dc.contributor.authorAlexandersson, Eriken_ZA
dc.contributor.authorBecker, Johnen_ZA
dc.contributor.authorJacobson, Danen_ZA
dc.contributor.authorNguema-Ona, Ericen_ZA
dc.contributor.authorSteyn, Cobusen_ZA
dc.contributor.authorDenby, Katherineen_ZA
dc.contributor.authorVivier, Melaneen_ZA
dc.date.accessioned2015-11-11T12:00:11Z
dc.date.available2015-11-11T12:00:11Z
dc.date.issued2011en_ZA
dc.description.abstractBACKGROUND: Polygalacturonase-inhibiting proteins (PGIPs) directly limit the effective ingress of fungal pathogens by inhibiting cell wall-degrading endopolygalacturonases (ePGs). Transgenic tobacco plants over-expressing grapevine (Vitis vinifera) Vvpgip1 have previously been shown to be resistant to Botrytis infection. In this study we characterized two of these PGIP over-expressing lines with known resistance phenotypes by gene expression and hormone profiling in the absence of pathogen infection. RESULTS: Global gene expression was performed by a cross-species microarray approach using a potato cDNA microarray. The degree of potential cross-hybridization between probes was modeled by a novel computational workflow designed in-house. Probe annotations were updated by predicting probe-to-transcript hybridizations and combining information derived from other plant species. Comparing uninfected Vvpgip1-overexpressing lines to wild-type (WT), 318 probes showed significant change in expression. Functional groups of genes involved in metabolism and associated to the cell wall were identified and consequent cell wall analysis revealed increased lignin-levels in the transgenic lines, but no major differences in cell wall-derived polysaccharides. GO enrichment analysis also identified genes responsive to auxin, which was supported by elevated indole-acetic acid (IAA) levels in the transgenic lines. Finally, a down-regulation of xyloglucan endotransglycosylase/hydrolases (XTHs), which are important in cell wall remodeling, was linked to a decrease in total XTH activity. CONCLUSIONS: This evaluation of PGIP over-expressing plants performed under pathogen-free conditions to exclude the classical PGIP-ePG inhibition interaction indicates additional roles for PGIPs beyond the inhibition of ePGs.en_ZA
dc.identifier.apacitationAlexandersson, E., Becker, J., Jacobson, D., Nguema-Ona, E., Steyn, C., Denby, K., & Vivier, M. (2011). Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco. <i>BMC Research Notes</i>, http://hdl.handle.net/11427/14883en_ZA
dc.identifier.chicagocitationAlexandersson, Erik, John Becker, Dan Jacobson, Eric Nguema-Ona, Cobus Steyn, Katherine Denby, and Melane Vivier "Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco." <i>BMC Research Notes</i> (2011) http://hdl.handle.net/11427/14883en_ZA
dc.identifier.citationAlexandersson, E., Becker, J. V., Jacobson, D., Nguema-Ona, E., Steyn, C., Denby, K. J., & Vivier, M. A. (2011). Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco. BMC research notes, 4(1), 493.en_ZA
dc.identifier.ris TY - Journal Article AU - Alexandersson, Erik AU - Becker, John AU - Jacobson, Dan AU - Nguema-Ona, Eric AU - Steyn, Cobus AU - Denby, Katherine AU - Vivier, Melane AB - BACKGROUND: Polygalacturonase-inhibiting proteins (PGIPs) directly limit the effective ingress of fungal pathogens by inhibiting cell wall-degrading endopolygalacturonases (ePGs). Transgenic tobacco plants over-expressing grapevine (Vitis vinifera) Vvpgip1 have previously been shown to be resistant to Botrytis infection. In this study we characterized two of these PGIP over-expressing lines with known resistance phenotypes by gene expression and hormone profiling in the absence of pathogen infection. RESULTS: Global gene expression was performed by a cross-species microarray approach using a potato cDNA microarray. The degree of potential cross-hybridization between probes was modeled by a novel computational workflow designed in-house. Probe annotations were updated by predicting probe-to-transcript hybridizations and combining information derived from other plant species. Comparing uninfected Vvpgip1-overexpressing lines to wild-type (WT), 318 probes showed significant change in expression. Functional groups of genes involved in metabolism and associated to the cell wall were identified and consequent cell wall analysis revealed increased lignin-levels in the transgenic lines, but no major differences in cell wall-derived polysaccharides. GO enrichment analysis also identified genes responsive to auxin, which was supported by elevated indole-acetic acid (IAA) levels in the transgenic lines. Finally, a down-regulation of xyloglucan endotransglycosylase/hydrolases (XTHs), which are important in cell wall remodeling, was linked to a decrease in total XTH activity. CONCLUSIONS: This evaluation of PGIP over-expressing plants performed under pathogen-free conditions to exclude the classical PGIP-ePG inhibition interaction indicates additional roles for PGIPs beyond the inhibition of ePGs. DA - 2011 DB - OpenUCT DO - 10.1186/1756-0500-4-493 DP - University of Cape Town J1 - BMC Research Notes LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco TI - Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco UR - http://hdl.handle.net/11427/14883 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14883
dc.identifier.urihttp://dx.doi.org/10.1186/1756-0500-4-493
dc.identifier.vancouvercitationAlexandersson E, Becker J, Jacobson D, Nguema-Ona E, Steyn C, Denby K, et al. Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco. BMC Research Notes. 2011; http://hdl.handle.net/11427/14883.en_ZA
dc.language.isoengen_ZA
dc.publisherBioMed Central Ltden_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.rights.holder2011 Alexandersson et al.; licensee BioMed Central Ltd.en_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_ZA
dc.sourceBMC Research Notesen_ZA
dc.source.urihttp://www.biomedcentral.com/bmcresnotes/en_ZA
dc.subject.otherCell Wall-associated Geneen_ZA
dc.subject.otherBotrytis Infectionen_ZA
dc.subject.otherTobacco Leaf Materialen_ZA
dc.subject.otherPotato Microarrayen_ZA
dc.subject.otherControl Tobacco Planten_ZA
dc.subject.otherPGIP Activityen_ZA
dc.subject.otherWT Planten_ZA
dc.subject.otherJA Levelen_ZA
dc.subject.otherSA Levelen_ZA
dc.subject.otherLignin Contenten_ZA
dc.subject.otherPowdery Mildewen_ZA
dc.subject.otherJA Signalinen_ZA
dc.titleConstitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobaccoen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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