Genome-wide DNA methylation in mixed ancestry individuals with diabetes and prediabetes from South Africa

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dc.contributor.authorMatsha, Tandi E
dc.contributor.authorPheiffer, Carmen
dc.contributor.authorHumphries, Stephen E
dc.contributor.authorGamieldien, Junaid
dc.contributor.authorErasmus, Rajiv T
dc.contributor.authorKengne, Andre P
dc.date.accessioned2021-10-08T07:04:20Z
dc.date.available2021-10-08T07:04:20Z
dc.date.issued2016
dc.description.abstractAims. To conduct a genome-wide DNA methylation in individuals with type 2 diabetes, individuals with prediabetes, and control mixed ancestry individuals from South Africa. Methods. We used peripheral blood to perform genome-wide DNA methylation analysis in 3 individuals with screen detected diabetes, 3 individuals with prediabetes, and 3 individuals with normoglycaemia from the Bellville South Community, Cape Town, South Africa, who were age-, gender-, body mass index-, and duration of residency-matched. Methylated DNA immunoprecipitation (MeDIP) was performed by Arraystar Inc. (Rockville, MD, USA). Results. Hypermethylated DMRs were 1160 (81.97%) and 124 (43.20%), respectively, in individuals with diabetes and prediabetes when both were compared to subjects with normoglycaemia. Our data shows that genes related to the immune system, signal transduction, glucose transport, and pancreas development have altered DNA methylation in subjects with prediabetes and diabetes. Pathway analysis based on the functional analysis mapping of genes to KEGG pathways suggested that the linoleic acid metabolism and arachidonic acid metabolism pathways are hypomethylated in prediabetes and diabetes. Conclusions. Our study suggests that epigenetic changes are likely to be an early process that occurs before the onset of overt diabetes. Detailed analysis of DMRs that shows gradual methylation differences from control versus prediabetes to prediabetes versus diabetes in a larger sample size is required to confirm these findings.
dc.identifier.apacitationMatsha, T. E., Pheiffer, C., Humphries, S. E., Gamieldien, J., Erasmus, R. T., & Kengne, A. P. (2016). Genome-wide DNA methylation in mixed ancestry individuals with diabetes and prediabetes from South Africa. <i>International Journal of Endocrinology</i>, 2016(4), 174 - 177. http://hdl.handle.net/11427/34484en_ZA
dc.identifier.chicagocitationMatsha, Tandi E, Carmen Pheiffer, Stephen E Humphries, Junaid Gamieldien, Rajiv T Erasmus, and Andre P Kengne "Genome-wide DNA methylation in mixed ancestry individuals with diabetes and prediabetes from South Africa." <i>International Journal of Endocrinology</i> 2016, 4. (2016): 174 - 177. http://hdl.handle.net/11427/34484en_ZA
dc.identifier.citationMatsha, T.E., Pheiffer, C., Humphries, S.E., Gamieldien, J., Erasmus, R.T. & Kengne, A.P. 2016. Genome-wide DNA methylation in mixed ancestry individuals with diabetes and prediabetes from South Africa. <i>International Journal of Endocrinology.</i> 2016(4):174 - 177. http://hdl.handle.net/11427/34484en_ZA
dc.identifier.issn1687-8337
dc.identifier.issn1687-8345
dc.identifier.ris TY - Journal Article AU - Matsha, Tandi E AU - Pheiffer, Carmen AU - Humphries, Stephen E AU - Gamieldien, Junaid AU - Erasmus, Rajiv T AU - Kengne, Andre P AB - Aims. To conduct a genome-wide DNA methylation in individuals with type 2 diabetes, individuals with prediabetes, and control mixed ancestry individuals from South Africa. Methods. We used peripheral blood to perform genome-wide DNA methylation analysis in 3 individuals with screen detected diabetes, 3 individuals with prediabetes, and 3 individuals with normoglycaemia from the Bellville South Community, Cape Town, South Africa, who were age-, gender-, body mass index-, and duration of residency-matched. Methylated DNA immunoprecipitation (MeDIP) was performed by Arraystar Inc. (Rockville, MD, USA). Results. Hypermethylated DMRs were 1160 (81.97%) and 124 (43.20%), respectively, in individuals with diabetes and prediabetes when both were compared to subjects with normoglycaemia. Our data shows that genes related to the immune system, signal transduction, glucose transport, and pancreas development have altered DNA methylation in subjects with prediabetes and diabetes. Pathway analysis based on the functional analysis mapping of genes to KEGG pathways suggested that the linoleic acid metabolism and arachidonic acid metabolism pathways are hypomethylated in prediabetes and diabetes. Conclusions. Our study suggests that epigenetic changes are likely to be an early process that occurs before the onset of overt diabetes. Detailed analysis of DMRs that shows gradual methylation differences from control versus prediabetes to prediabetes versus diabetes in a larger sample size is required to confirm these findings. DA - 2016 DB - OpenUCT DP - University of Cape Town IS - 4 J1 - International Journal of Endocrinology LK - https://open.uct.ac.za PY - 2016 SM - 1687-8337 SM - 1687-8345 T1 - Genome-wide DNA methylation in mixed ancestry individuals with diabetes and prediabetes from South Africa TI - Genome-wide DNA methylation in mixed ancestry individuals with diabetes and prediabetes from South Africa UR - http://hdl.handle.net/11427/34484 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/34484
dc.identifier.vancouvercitationMatsha TE, Pheiffer C, Humphries SE, Gamieldien J, Erasmus RT, Kengne AP. Genome-wide DNA methylation in mixed ancestry individuals with diabetes and prediabetes from South Africa. International Journal of Endocrinology. 2016;2016(4):174 - 177. http://hdl.handle.net/11427/34484.en_ZA
dc.language.isoeng
dc.publisher.departmentDepartment of Medicine
dc.publisher.facultyFaculty of Health Sciences
dc.sourceInternational Journal of Endocrinology
dc.source.journalissue4
dc.source.journalvolume2016
dc.source.pagination174 - 177
dc.source.urihttps://dx.doi.org/10.1155/2016/3172093
dc.subject.otherScience &
dc.subject.otherTechnology
dc.subject.otherLife Sciences &
dc.subject.otherBiomedicine
dc.subject.otherEndocrinology &
dc.subject.otherMetabolism
dc.subject.otherFATTY-ACIDS
dc.subject.otherMELLITUS
dc.subject.otherMETABOLITES
dc.subject.otherPREVALENCE
dc.subject.otherLIPIDOMICS
dc.subject.otherBELLVILLE
dc.subject.otherGENES
dc.subject.otherBLOOD
dc.titleGenome-wide DNA methylation in mixed ancestry individuals with diabetes and prediabetes from South Africa
dc.typeJournal Article
uct.type.publicationResearch
uct.type.resourceJournal Article
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