A 40-50kDa Glycoprotein Associated with Mucus is Identified as α-1-Acid Glycoprotein in Carcinoma of the Stomach

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dc.contributor.authorChirwa, Nthato
dc.contributor.authorGovender, Dhirendra
dc.contributor.authorNdimba, Bongani
dc.contributor.authorLotz, Zoe
dc.contributor.authorTyler, Marilyn
dc.contributor.authorPanieri, Eugenio
dc.contributor.authorKAHN, DELAWIR
dc.contributor.authorMall, Anwar S
dc.date.accessioned2021-10-08T07:04:25Z
dc.date.available2021-10-08T07:04:25Z
dc.date.issued2012
dc.description.abstractBackground and Aim: Secreted gastric mucins are large O-glycosylated proteins of crude mucus gels which are aberrantly expressed in malignancy. An albumin associated 55-65kDa glycoprotein was previously shown in mucus gels in gastric cancer. The aim of this study was to investigate its expression and identification in human gastric tissue. Methods: Mucins were purified from crude mucus scrapings of 16 partial and 11 total resections and a rabbit polyclonal antibody was raised to the 55-65kDa glycoprotein. The location and expression of the glycoprotein was examined in normal gastric mucosa (n=20), intestinal metaplasia (n=18) and gastric cancer (n=27) tissue by immunohistochemistry. Mucins were analyzed by isoelectric focusing (IEF) on 2-D polyacrylamide gels. Identification of the 40-50kDa glycoprotein was by MALDI-TOF MS technique. Plasma levels were examined by Western blotting. Results: Extensive SDS-PAGE analysis gave a PAS positive glycoprotein in the 40-50kDa range, in patients with gastric cancer but not normals. It was expressed in parietal and columnar cells of normal gastric tissue and intestinal metaplasia respectively, and in 22 of 27 gastric cancer specimens. In 2-D PAGE stained with Coomassie Blue there were 3 spots positively identified as alpha-1-acid glycoprotein (AGP) by MALDI-TOF MS technique. PAS staining revealed a single bright spot in the same position but could not be identified. Preliminary measurements showed slightly higher levels of AGP in plasma of patients with gastric carcinoma. Conclusion: AGP levels are increased in gastric tissue and in the plasma of those with carcinoma of the stomach.
dc.identifier.apacitationChirwa, N., Govender, D., Ndimba, B., Lotz, Z., Tyler, M., Panieri, E., ... Mall, A. S. (2012). A 40-50kDa Glycoprotein Associated with Mucus is Identified as α-1-Acid Glycoprotein in Carcinoma of the Stomach. <i>Journal of Cancer</i>, 3(4), 83 - 92. http://hdl.handle.net/11427/34502en_ZA
dc.identifier.chicagocitationChirwa, Nthato, Dhirendra Govender, Bongani Ndimba, Zoe Lotz, Marilyn Tyler, Eugenio Panieri, DELAWIR KAHN, and Anwar S Mall "A 40-50kDa Glycoprotein Associated with Mucus is Identified as α-1-Acid Glycoprotein in Carcinoma of the Stomach." <i>Journal of Cancer</i> 3, 4. (2012): 83 - 92. http://hdl.handle.net/11427/34502en_ZA
dc.identifier.citationChirwa, N., Govender, D., Ndimba, B., Lotz, Z., Tyler, M., Panieri, E., KAHN, D. & Mall, A.S. et al. 2012. A 40-50kDa Glycoprotein Associated with Mucus is Identified as α-1-Acid Glycoprotein in Carcinoma of the Stomach. <i>Journal of Cancer.</i> 3(4):83 - 92. http://hdl.handle.net/11427/34502en_ZA
dc.identifier.issn1837-9664
dc.identifier.ris TY - Journal Article AU - Chirwa, Nthato AU - Govender, Dhirendra AU - Ndimba, Bongani AU - Lotz, Zoe AU - Tyler, Marilyn AU - Panieri, Eugenio AU - KAHN, DELAWIR AU - Mall, Anwar S AB - Background and Aim: Secreted gastric mucins are large O-glycosylated proteins of crude mucus gels which are aberrantly expressed in malignancy. An albumin associated 55-65kDa glycoprotein was previously shown in mucus gels in gastric cancer. The aim of this study was to investigate its expression and identification in human gastric tissue. Methods: Mucins were purified from crude mucus scrapings of 16 partial and 11 total resections and a rabbit polyclonal antibody was raised to the 55-65kDa glycoprotein. The location and expression of the glycoprotein was examined in normal gastric mucosa (n=20), intestinal metaplasia (n=18) and gastric cancer (n=27) tissue by immunohistochemistry. Mucins were analyzed by isoelectric focusing (IEF) on 2-D polyacrylamide gels. Identification of the 40-50kDa glycoprotein was by MALDI-TOF MS technique. Plasma levels were examined by Western blotting. Results: Extensive SDS-PAGE analysis gave a PAS positive glycoprotein in the 40-50kDa range, in patients with gastric cancer but not normals. It was expressed in parietal and columnar cells of normal gastric tissue and intestinal metaplasia respectively, and in 22 of 27 gastric cancer specimens. In 2-D PAGE stained with Coomassie Blue there were 3 spots positively identified as alpha-1-acid glycoprotein (AGP) by MALDI-TOF MS technique. PAS staining revealed a single bright spot in the same position but could not be identified. Preliminary measurements showed slightly higher levels of AGP in plasma of patients with gastric carcinoma. Conclusion: AGP levels are increased in gastric tissue and in the plasma of those with carcinoma of the stomach. DA - 2012 DB - OpenUCT DP - University of Cape Town IS - 4 J1 - Journal of Cancer LK - https://open.uct.ac.za PY - 2012 SM - 1837-9664 T1 - A 40-50kDa Glycoprotein Associated with Mucus is Identified as α-1-Acid Glycoprotein in Carcinoma of the Stomach TI - A 40-50kDa Glycoprotein Associated with Mucus is Identified as α-1-Acid Glycoprotein in Carcinoma of the Stomach UR - http://hdl.handle.net/11427/34502 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/34502
dc.identifier.vancouvercitationChirwa N, Govender D, Ndimba B, Lotz Z, Tyler M, Panieri E, et al. A 40-50kDa Glycoprotein Associated with Mucus is Identified as α-1-Acid Glycoprotein in Carcinoma of the Stomach. Journal of Cancer. 2012;3(4):83 - 92. http://hdl.handle.net/11427/34502.en_ZA
dc.publisher.departmentDepartment of Surgery
dc.publisher.facultyFaculty of Health Sciences
dc.sourceJournal of Cancer
dc.source.journalissue4
dc.source.journalvolume3
dc.source.pagination83 - 92
dc.source.urihttps://dx.doi.org/10.7150/jca.3737
dc.subject.otherMucin
dc.subject.otherMucus
dc.subject.othercancer
dc.subject.othergastric
dc.subject.otherintestinal metaplasia
dc.subject.otherα-1-acid-glycoprotein.
dc.titleA 40-50kDa Glycoprotein Associated with Mucus is Identified as α-1-Acid Glycoprotein in Carcinoma of the Stomach
dc.typeJournal Article
uct.type.publicationResearch
uct.type.resourceJournal Article
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