The effect of As(III) and As(V) on the batch bioleaching of a pyrite-arsenopyrite concentrate
| dc.contributor.author | Breed, A W | |
| dc.contributor.author | Glatz, A | |
| dc.contributor.author | Hansford, G S | |
| dc.contributor.author | Harrison, S T L | |
| dc.date.accessioned | 2016-08-17T08:32:42Z | |
| dc.date.available | 2016-08-17T08:32:42Z | |
| dc.date.issued | 1996 | |
| dc.date.updated | 2016-08-16T10:18:40Z | |
| dc.description.abstract | The bioleaching of arsenical gold-bearing sulphide ores and concentrates solubilises iron, arsenic and sulphur. Previous work has shown that high concentrations of iron and arsenic in solution inhibit bacterial growth, with As(III) reported to inhibit bacteria to a greater degree than As(V). Batch bioleaching experiments were carried out over periods of one month. Varying quantities of either 0.020–0.040 M As(III) or 0.107–0.220 M As(V), were added to a slurry, consisting of a pyrite-arsenopyrite concentrate (20% solids (m.v−1)) in a nutrient solution. The slurry was inoculated with a culture, consisting primarily of Leptospirillum ferrooxidans and Thiobacillus thiooxidans. The culture was obtained from a continuous bioleaching mini-plant treating the same concentrate. The results obtained were compared with those of a culture to which no arsenic was added. The effect of the added arsenic was determined by monitoring three parameters: the oxygen utilisation rate, rO2, of the culture, the rate at which the arsenic in the concentrate was solubilised and the speciation of the dissolved arsenic. The results suggest that the nature of the As(III) and As(V) toxicity is different. The addition of the culture to a slurry containing As(III) resulted in a reduced rate of bacterial oxidation. However, the addition of the culture to a slurry containing As(V) resulted in both a lag phase and a reduced rate of bacterial oxidation. At sufficiently high dosages of As(III) and As(V) the maximum oxygen utilisation rate, rO2max, of the culture was also affected. The results indicate that As(V) toxicity, and the relative toxicity of As(III) and As(V) to a mixed culture, appear to be affected by the availability of an energy source. Hence the toxicity of As(III) is not necessarily in the region of three times that of As(V). Furthermore, the results suggest that the mechanism of arsenic resistance may be attributed to the Pst+ Pit− mutations and an energy dependent efflux pump. | en_ZA |
| dc.identifier | http://dx.doi.org/10.1016/S0892-6875(96)00119-7 | |
| dc.identifier.apacitation | Breed, A. W., Glatz, A., Hansford, G. S., & Harrison, S. T. L. (1996). The effect of As(III) and As(V) on the batch bioleaching of a pyrite-arsenopyrite concentrate. <i>Minerals Engineering</i>, http://hdl.handle.net/11427/21279 | en_ZA |
| dc.identifier.chicagocitation | Breed, A W, A Glatz, G S Hansford, and S T L Harrison "The effect of As(III) and As(V) on the batch bioleaching of a pyrite-arsenopyrite concentrate." <i>Minerals Engineering</i> (1996) http://hdl.handle.net/11427/21279 | en_ZA |
| dc.identifier.citation | Breed, A. W., Glatz, A., Hansford, G. S., & Harrison, S. T. L. (1996). The effect of As (III) and As (V) on the batch bioleaching of a pyrite-arsenopyrite concentrate. Minerals Engineering, 9(12), 1235-1252. | en_ZA |
| dc.identifier.issn | 0892-6875 | en_ZA |
| dc.identifier.ris | TY - Journal Article AU - Breed, A W AU - Glatz, A AU - Hansford, G S AU - Harrison, S T L AB - The bioleaching of arsenical gold-bearing sulphide ores and concentrates solubilises iron, arsenic and sulphur. Previous work has shown that high concentrations of iron and arsenic in solution inhibit bacterial growth, with As(III) reported to inhibit bacteria to a greater degree than As(V). Batch bioleaching experiments were carried out over periods of one month. Varying quantities of either 0.020–0.040 M As(III) or 0.107–0.220 M As(V), were added to a slurry, consisting of a pyrite-arsenopyrite concentrate (20% solids (m.v−1)) in a nutrient solution. The slurry was inoculated with a culture, consisting primarily of Leptospirillum ferrooxidans and Thiobacillus thiooxidans. The culture was obtained from a continuous bioleaching mini-plant treating the same concentrate. The results obtained were compared with those of a culture to which no arsenic was added. The effect of the added arsenic was determined by monitoring three parameters: the oxygen utilisation rate, rO2, of the culture, the rate at which the arsenic in the concentrate was solubilised and the speciation of the dissolved arsenic. The results suggest that the nature of the As(III) and As(V) toxicity is different. The addition of the culture to a slurry containing As(III) resulted in a reduced rate of bacterial oxidation. However, the addition of the culture to a slurry containing As(V) resulted in both a lag phase and a reduced rate of bacterial oxidation. At sufficiently high dosages of As(III) and As(V) the maximum oxygen utilisation rate, rO2max, of the culture was also affected. The results indicate that As(V) toxicity, and the relative toxicity of As(III) and As(V) to a mixed culture, appear to be affected by the availability of an energy source. Hence the toxicity of As(III) is not necessarily in the region of three times that of As(V). Furthermore, the results suggest that the mechanism of arsenic resistance may be attributed to the Pst+ Pit− mutations and an energy dependent efflux pump. DA - 1996 DB - OpenUCT DP - University of Cape Town J1 - Minerals Engineering LK - https://open.uct.ac.za PB - University of Cape Town PY - 1996 SM - 0892-6875 T1 - The effect of As(III) and As(V) on the batch bioleaching of a pyrite-arsenopyrite concentrate TI - The effect of As(III) and As(V) on the batch bioleaching of a pyrite-arsenopyrite concentrate UR - http://hdl.handle.net/11427/21279 ER - | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/21279 | |
| dc.identifier.vancouvercitation | Breed AW, Glatz A, Hansford GS, Harrison STL. The effect of As(III) and As(V) on the batch bioleaching of a pyrite-arsenopyrite concentrate. Minerals Engineering. 1996; http://hdl.handle.net/11427/21279. | en_ZA |
| dc.language | eng | en_ZA |
| dc.publisher | Elsevier | en_ZA |
| dc.publisher.institution | University of Cape Town | |
| dc.rights | Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_ZA |
| dc.source | Minerals Engineering | en_ZA |
| dc.source.uri | http://www.journals.elsevier.com/minerals-engineering/ | |
| dc.subject.other | Bacteria | |
| dc.subject.other | bioleaching | |
| dc.subject.other | oxidation | |
| dc.subject.other | sulphide ores | |
| dc.subject.other | extractive metallurgy | |
| dc.title | The effect of As(III) and As(V) on the batch bioleaching of a pyrite-arsenopyrite concentrate | en_ZA |
| dc.type | Journal Article | en_ZA |
| uct.type.filetype | Text | |
| uct.type.filetype | Image | |
| uct.type.publication | Research | en_ZA |
| uct.type.resource | Article | en_ZA |