The effect of nutrient supplementation on growth and leaching performance of bioleaching bacteria

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dc.contributor.authorvan Hille, Robert P
dc.contributor.authorBromfield, L V
dc.contributor.authorBotha, S S
dc.contributor.authorJones, G
dc.contributor.authorvan Zyl, A W
dc.contributor.authorHarrison, Susan T L
dc.date.accessioned2016-08-19T11:32:47Z
dc.date.available2016-08-19T11:32:47Z
dc.date.issued2009
dc.date.updated2016-08-17T12:26:06Z
dc.description.abstractHeap bioleaching operations are often faced with extended and unpredictable lag periods after inoculation, prior to the establishment of a stable oxidising environment, during which the heap is fully colonised or the inoculum overcomes the sub-optimal conditions resulting from acid agglomeration. Supplementation of laboratory scale (4kg ore) leach columns with soluble nitrogen, particularly as yeast extract, significantly reduced the lag time and promoted bacterial growth, resulting in a 50-95% increase in copper recovery post-inoculation. The effect of yeast extract addition to Acidithiobacillus ferrooxidans in controlled oxidation tests was investigated. Initial exposure of a stock culture to yeast extract resulted in a transient, dose dependent inhibition at concentrations of 0.5 g.l-1 and below. At 1.25 g.l-1 inhibition was complete over the time scale of the experiment. The inhibition phase was characterised by observable changes in cell morphology and ultrastructure. Despite the initial inhibition, the biomass yield at the end of the experiments was equivalent, or higher, in the presence of yeast extract. Cultures were adapted to growth on yeast extract as the sole nitrogen source and adapted cultures showed the highest rates of iron oxidation and cell growth, in the presence of 0.5 and 1 g.l-1 of yeast extract.en_ZA
dc.identifier.apacitationvan Hille, R. P., Bromfield, L. V., Botha, S. S., Jones, G., van Zyl, A. W., & Harrison, S. T. L. (2009). The effect of nutrient supplementation on growth and leaching performance of bioleaching bacteria. <i>Advanced Materials Research</i>, http://hdl.handle.net/11427/21358en_ZA
dc.identifier.chicagocitationvan Hille, Robert P, L V Bromfield, S S Botha, G Jones, A W van Zyl, and Susan T L Harrison "The effect of nutrient supplementation on growth and leaching performance of bioleaching bacteria." <i>Advanced Materials Research</i> (2009) http://hdl.handle.net/11427/21358en_ZA
dc.identifier.citationvan Hille, R. P., Bromfield, L. V., Botha, S. S., Jones, G., van Zyl, A. W., & Harrison, S. T. (2009). The effect of nutrient supplementation on growth and leaching performance of bioleaching bacteria. In Advanced Materials Research (Vol. 71, pp. 413-416). Trans Tech Publications.en_ZA
dc.identifier.issn1022-6680en_ZA
dc.identifier.ris TY - Journal Article AU - van Hille, Robert P AU - Bromfield, L V AU - Botha, S S AU - Jones, G AU - van Zyl, A W AU - Harrison, Susan T L AB - Heap bioleaching operations are often faced with extended and unpredictable lag periods after inoculation, prior to the establishment of a stable oxidising environment, during which the heap is fully colonised or the inoculum overcomes the sub-optimal conditions resulting from acid agglomeration. Supplementation of laboratory scale (4kg ore) leach columns with soluble nitrogen, particularly as yeast extract, significantly reduced the lag time and promoted bacterial growth, resulting in a 50-95% increase in copper recovery post-inoculation. The effect of yeast extract addition to Acidithiobacillus ferrooxidans in controlled oxidation tests was investigated. Initial exposure of a stock culture to yeast extract resulted in a transient, dose dependent inhibition at concentrations of 0.5 g.l-1 and below. At 1.25 g.l-1 inhibition was complete over the time scale of the experiment. The inhibition phase was characterised by observable changes in cell morphology and ultrastructure. Despite the initial inhibition, the biomass yield at the end of the experiments was equivalent, or higher, in the presence of yeast extract. Cultures were adapted to growth on yeast extract as the sole nitrogen source and adapted cultures showed the highest rates of iron oxidation and cell growth, in the presence of 0.5 and 1 g.l-1 of yeast extract. DA - 2009 DB - OpenUCT DP - University of Cape Town J1 - Advanced Materials Research LK - https://open.uct.ac.za PB - University of Cape Town PY - 2009 SM - 1022-6680 T1 - The effect of nutrient supplementation on growth and leaching performance of bioleaching bacteria TI - The effect of nutrient supplementation on growth and leaching performance of bioleaching bacteria UR - http://hdl.handle.net/11427/21358 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21358
dc.identifier.vancouvercitationvan Hille RP, Bromfield LV, Botha SS, Jones G, van Zyl AW, Harrison STL. The effect of nutrient supplementation on growth and leaching performance of bioleaching bacteria. Advanced Materials Research. 2009; http://hdl.handle.net/11427/21358.en_ZA
dc.languageengen_ZA
dc.publisherTrans Tech Publicationsen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceAdvanced Materials Researchen_ZA
dc.source.urihttp://www.scientific.net/AMR
dc.titleThe effect of nutrient supplementation on growth and leaching performance of bioleaching bacteriaen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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