Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize

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dc.contributor.advisorVon Wechmar, M Barbaraen_ZA
dc.contributor.authorKnox, Elizabethen_ZA
dc.date.accessioned2016-09-25T16:46:58Z
dc.date.available2016-09-25T16:46:58Z
dc.date.issued1986en_ZA
dc.descriptionBibliography : pages 218-230.en_ZA
dc.description.abstractMaize plants collected in three geographically distinct regions of South Africa were found to be doubly infected with maize dwarf mosaic (MDMV) and cucumber mosaic virus (CMV). A mixed infection of these two viruses could be maintained in maize plants grown under laboratory conditions. The possibility of synergism or of an interference mechanism between MDMV and CMV in dual infections was investigated and it was found that prior infection with CMV interfered with subsequent infection by MDMV. MDMV and CMV were shown to be non-persistently transmitted by Myzus persicae, Rhopalosiphum padi and Rhopalosipbum maidis aphids. Protoplasts were isolated from maize seedlings and could be viably maintained for up to 66 hours. The maize protoplasts were infected with CMV and MDMV either singly, or together as a mixed inoculum. Infection curves for each virus were plotted. The presence of CMV in a mixed inoculum appeared to prevent infection of the protoplasts by MDMV. Protoplasts were isolated from plants systemically infected with CMV and/or MDMV. Superinfection of protoplasts prepared from CMV infected seedlings with MDMV was not possible. As a possible vehicle for virus infection of protoplasts liposomes were produced. Initially fluorescent dyes were incorporated in them. These were fused to the maize protoplasts. Attempts were made to encapsulate virus particles in the liposomes and fuse them to maize protoplasts but this was not successful.en_ZA
dc.identifier.apacitationKnox, E. (1986). <i>Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/21898en_ZA
dc.identifier.chicagocitationKnox, Elizabeth. <i>"Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1986. http://hdl.handle.net/11427/21898en_ZA
dc.identifier.citationKnox, E. 1986. Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Knox, Elizabeth AB - Maize plants collected in three geographically distinct regions of South Africa were found to be doubly infected with maize dwarf mosaic (MDMV) and cucumber mosaic virus (CMV). A mixed infection of these two viruses could be maintained in maize plants grown under laboratory conditions. The possibility of synergism or of an interference mechanism between MDMV and CMV in dual infections was investigated and it was found that prior infection with CMV interfered with subsequent infection by MDMV. MDMV and CMV were shown to be non-persistently transmitted by Myzus persicae, Rhopalosiphum padi and Rhopalosipbum maidis aphids. Protoplasts were isolated from maize seedlings and could be viably maintained for up to 66 hours. The maize protoplasts were infected with CMV and MDMV either singly, or together as a mixed inoculum. Infection curves for each virus were plotted. The presence of CMV in a mixed inoculum appeared to prevent infection of the protoplasts by MDMV. Protoplasts were isolated from plants systemically infected with CMV and/or MDMV. Superinfection of protoplasts prepared from CMV infected seedlings with MDMV was not possible. As a possible vehicle for virus infection of protoplasts liposomes were produced. Initially fluorescent dyes were incorporated in them. These were fused to the maize protoplasts. Attempts were made to encapsulate virus particles in the liposomes and fuse them to maize protoplasts but this was not successful. DA - 1986 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1986 T1 - Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize TI - Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize UR - http://hdl.handle.net/11427/21898 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21898
dc.identifier.vancouvercitationKnox E. Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1986 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/21898en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMicrobiologyen_ZA
dc.titleMixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maizeen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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