The performance of HIV rapid antibody detection assays in children

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dc.contributor.advisorEley, Brianen_ZA
dc.contributor.authorNuttall, James Jolyon Careen_ZA
dc.date.accessioned2016-07-11T13:50:56Z
dc.date.available2016-07-11T13:50:56Z
dc.date.issued2016en_ZA
dc.description.abstractBackground: HIV rapid antibody assays are important for screening children aged <18 months for HIV exposure and children ≥ 18 months for HIV infection. Limited available data indicate variable performance of different HIV rapid tests in comparison to laboratory HIV antibody assays. The aim of this study was to evaluate the diagnostic accuracy of 6 HIV rapid tests currently used in South Africa for screening children using whole blood. Methods: A prospective descriptive cross-sectional laboratory study was conducted at two paediatric healthcare facilities in South Africa. Sensitivity and specificity analyses and positive and negative likelihood ratios were performed. The reference standard was the laboratory HIV enzyme-linked immunosorbent assay (ELISA) test and HIV polymerase chain reaction (PCR) test. Results: Blood samples from 1159 children (896 <18 months of age) with valid HIV ELISA test results were included in the analysis. A total of 5768 HIV rapid tests (4446 in children <18 months of age) were performed. Sensitivity of HIV rapid tests for detecting HIV exposure among children <18 months of age ranged from 38.7% to 94.7%. Four HIV rapid tests attained specificity in excluding HIV exposure among children <18 months of age of >98%. Seroreversion rates were lowest with the Determine rapid test. Three HIV rapid tests (Abon, Advanced Quality, Determine) detected 100% of HIV-infected children <18 months of age, the Reveal, SD Bioline and Insti rapid tests missed 27 (41.5%), 1 (4.5%) and 1 (1.5%) of the HIV-infected children respectively. In children ≥ 18 months of age, sensitivity of rapid tests for detecting HIV infection ranged from 69.2% to 100% and specificity of all rapid tests was 100%. Conclusions: None of the 6 HIV rapid tests evaluated achieved both the World Health Organisation recommended sensitivity and specificity standards for any antibody assay used in screening for HIV exposure in children <18 months. The Determine test showed the best overall diagnostic accuracy and is therefore recommended as the preferred screening test for children. Recommendations: on the use of specific HIV rapid tests in infants and young children should be based on evaluation of their performance in the population to be tested.en_ZA
dc.identifier.apacitationNuttall, J. J. C. (2016). <i>The performance of HIV rapid antibody detection assays in children</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Department of Paediatrics and Child Health. Retrieved from http://hdl.handle.net/11427/20296en_ZA
dc.identifier.chicagocitationNuttall, James Jolyon Care. <i>"The performance of HIV rapid antibody detection assays in children."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Department of Paediatrics and Child Health, 2016. http://hdl.handle.net/11427/20296en_ZA
dc.identifier.citationNuttall, J. 2016. The performance of HIV rapid antibody detection assays in children. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Nuttall, James Jolyon Care AB - Background: HIV rapid antibody assays are important for screening children aged <18 months for HIV exposure and children ≥ 18 months for HIV infection. Limited available data indicate variable performance of different HIV rapid tests in comparison to laboratory HIV antibody assays. The aim of this study was to evaluate the diagnostic accuracy of 6 HIV rapid tests currently used in South Africa for screening children using whole blood. Methods: A prospective descriptive cross-sectional laboratory study was conducted at two paediatric healthcare facilities in South Africa. Sensitivity and specificity analyses and positive and negative likelihood ratios were performed. The reference standard was the laboratory HIV enzyme-linked immunosorbent assay (ELISA) test and HIV polymerase chain reaction (PCR) test. Results: Blood samples from 1159 children (896 <18 months of age) with valid HIV ELISA test results were included in the analysis. A total of 5768 HIV rapid tests (4446 in children <18 months of age) were performed. Sensitivity of HIV rapid tests for detecting HIV exposure among children <18 months of age ranged from 38.7% to 94.7%. Four HIV rapid tests attained specificity in excluding HIV exposure among children <18 months of age of >98%. Seroreversion rates were lowest with the Determine rapid test. Three HIV rapid tests (Abon, Advanced Quality, Determine) detected 100% of HIV-infected children <18 months of age, the Reveal, SD Bioline and Insti rapid tests missed 27 (41.5%), 1 (4.5%) and 1 (1.5%) of the HIV-infected children respectively. In children ≥ 18 months of age, sensitivity of rapid tests for detecting HIV infection ranged from 69.2% to 100% and specificity of all rapid tests was 100%. Conclusions: None of the 6 HIV rapid tests evaluated achieved both the World Health Organisation recommended sensitivity and specificity standards for any antibody assay used in screening for HIV exposure in children <18 months. The Determine test showed the best overall diagnostic accuracy and is therefore recommended as the preferred screening test for children. Recommendations: on the use of specific HIV rapid tests in infants and young children should be based on evaluation of their performance in the population to be tested. DA - 2016 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2016 T1 - The performance of HIV rapid antibody detection assays in children TI - The performance of HIV rapid antibody detection assays in children UR - http://hdl.handle.net/11427/20296 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20296
dc.identifier.vancouvercitationNuttall JJC. The performance of HIV rapid antibody detection assays in children. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Department of Paediatrics and Child Health, 2016 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/20296en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Paediatrics and Child Healthen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherPaediatricsen_ZA
dc.titleThe performance of HIV rapid antibody detection assays in childrenen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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