Detection of Streptococcus pneumoniae from different types of nasopharyngeal swabs in children

Simple item page
dc.contributor.authorDube, Felix Sen_ZA
dc.contributor.authorKaba, Mamadouen_ZA
dc.contributor.authorWhittaker, Elizabethen_ZA
dc.contributor.authorZar, Heather Jen_ZA
dc.contributor.authorNicol, Mark Pen_ZA
dc.date.accessioned2015-12-28T06:51:42Z
dc.date.available2015-12-28T06:51:42Z
dc.date.issued2013en_ZA
dc.description.abstractBACKGROUND: A better understanding of the epidemiology of nasopharyngeal carriage of Streptococcus pneumoniae is important to assess the impact of vaccination and the pathogenesis of pneumococcal disease. We compared the recovery of S. pneumoniae from nylon flocked, Dacron and rayon swabs. METHODS: The recovery of S. pneumoniae from mocked specimens using flocked, Dacron and rayon swabs were compared by culture. The yield from paired nasopharyngeal (NP) samples obtained from healthy children sampled with flocked and Dacron swabs was also determined using culture and lytA -targeted real-time polymerase chain reaction (qPCR). RESULTS: Using mock specimen, the percentage recovery of S. pneumoniae ATCC 49619 (serotype 19F) strain from the flocked swabs was 100%, while it was 41% from Dacron swabs and 7% from rayon swabs. Similar results were observed for S. pneumoniae serotypes 1 and 5. S. pneumoniae was cultured from 18 of 42 (43%) paired NP samples from the healthy children (median age 8 [interquartile range (IQR) 5-16] months). The median number of colony-forming units (CFU) recovered from flocked swabs was two-fold higher (8.8×10 4 CFU/mL [IQR, 2.0×10 2 - 4.0×10 5 CFU/mL]) than Dacron swabs (3.7×10 4 CFU/mL [IQR, 4.0×10 2 -3.2×10 5 CFU/mL], p  = 0.17). Using lytA -targeted qPCR from paired NP samples, the median copy number of S. pneumoniae detected from flocked swabs was significantly higher than from Dacron swabs (3.0×10 5 genome copies/mL [IQR, 1.3×10 2 −1.8×10 6 ] vs. 9.3×10 4 genome copies/mL [IQR, 7.0×10 1 −1.1×10 6 ]; p  = 0.005). CONCLUSION: Flocked swabs released more S. pneumoniae compared to both Dacron and rayon swabs from mock specimens. Similarly, higher bacterial loads were detected by qPCR from flocked swabs compared with Dacron swabs from healthy children.en_ZA
dc.identifier.apacitationDube, F. S., Kaba, M., Whittaker, E., Zar, H. J., & Nicol, M. P. (2013). Detection of Streptococcus pneumoniae from different types of nasopharyngeal swabs in children. <i>PLoS One</i>, http://hdl.handle.net/11427/16069en_ZA
dc.identifier.chicagocitationDube, Felix S, Mamadou Kaba, Elizabeth Whittaker, Heather J Zar, and Mark P Nicol "Detection of Streptococcus pneumoniae from different types of nasopharyngeal swabs in children." <i>PLoS One</i> (2013) http://hdl.handle.net/11427/16069en_ZA
dc.identifier.citationDube, F. S., Kaba, M., Whittaker, E., Zar, H. J., & Nicol, M. P. (2013). Detection of Streptococcus pneumoniae from different types of nasopharyngeal swabs in children. PloS one, 8(6), e68097. doi:10.1371/journal.pone.0068097en_ZA
dc.identifier.ris TY - Journal Article AU - Dube, Felix S AU - Kaba, Mamadou AU - Whittaker, Elizabeth AU - Zar, Heather J AU - Nicol, Mark P AB - BACKGROUND: A better understanding of the epidemiology of nasopharyngeal carriage of Streptococcus pneumoniae is important to assess the impact of vaccination and the pathogenesis of pneumococcal disease. We compared the recovery of S. pneumoniae from nylon flocked, Dacron and rayon swabs. METHODS: The recovery of S. pneumoniae from mocked specimens using flocked, Dacron and rayon swabs were compared by culture. The yield from paired nasopharyngeal (NP) samples obtained from healthy children sampled with flocked and Dacron swabs was also determined using culture and lytA -targeted real-time polymerase chain reaction (qPCR). RESULTS: Using mock specimen, the percentage recovery of S. pneumoniae ATCC 49619 (serotype 19F) strain from the flocked swabs was 100%, while it was 41% from Dacron swabs and 7% from rayon swabs. Similar results were observed for S. pneumoniae serotypes 1 and 5. S. pneumoniae was cultured from 18 of 42 (43%) paired NP samples from the healthy children (median age 8 [interquartile range (IQR) 5-16] months). The median number of colony-forming units (CFU) recovered from flocked swabs was two-fold higher (8.8×10 4 CFU/mL [IQR, 2.0×10 2 - 4.0×10 5 CFU/mL]) than Dacron swabs (3.7×10 4 CFU/mL [IQR, 4.0×10 2 -3.2×10 5 CFU/mL], p  = 0.17). Using lytA -targeted qPCR from paired NP samples, the median copy number of S. pneumoniae detected from flocked swabs was significantly higher than from Dacron swabs (3.0×10 5 genome copies/mL [IQR, 1.3×10 2 −1.8×10 6 ] vs. 9.3×10 4 genome copies/mL [IQR, 7.0×10 1 −1.1×10 6 ]; p  = 0.005). CONCLUSION: Flocked swabs released more S. pneumoniae compared to both Dacron and rayon swabs from mock specimens. Similarly, higher bacterial loads were detected by qPCR from flocked swabs compared with Dacron swabs from healthy children. DA - 2013 DB - OpenUCT DO - 10.1371/journal.pone.0068097 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2013 T1 - Detection of Streptococcus pneumoniae from different types of nasopharyngeal swabs in children TI - Detection of Streptococcus pneumoniae from different types of nasopharyngeal swabs in children UR - http://hdl.handle.net/11427/16069 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/16069
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0068097
dc.identifier.vancouvercitationDube FS, Kaba M, Whittaker E, Zar HJ, Nicol MP. Detection of Streptococcus pneumoniae from different types of nasopharyngeal swabs in children. PLoS One. 2013; http://hdl.handle.net/11427/16069.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentDivision of Medical Biochemistryen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2013 Dube et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plosoneen_ZA
dc.subject.otherChildrenen_ZA
dc.subject.otherChild healthen_ZA
dc.subject.otherPolymerase chain reactionen_ZA
dc.subject.otherPneumococcusen_ZA
dc.subject.otherNucleic acidsen_ZA
dc.subject.otherPneumoniaen_ZA
dc.subject.otherDNA extractionen_ZA
dc.subject.otherHealth services researchen_ZA
dc.titleDetection of Streptococcus pneumoniae from different types of nasopharyngeal swabs in childrenen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Dube_Detection_of_Streptococcus_pneumoniae_2013.pdf
Size:
184.41 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Journal Articles