An oral recombinant Salmonella enterica serovar Typhimurium mutant elicits systemic antigen-specific CD8+ T cell cytokine responses in mice

dc.contributor.authorChin'ombe, Nyashaen_ZA
dc.contributor.authorBourn, Williamen_ZA
dc.contributor.authorWilliamson, Anna-Liseen_ZA
dc.contributor.authorShephard, Eniden_ZA
dc.date.accessioned2015-10-28T07:01:08Z
dc.date.available2015-10-28T07:01:08Z
dc.date.issued2009en_ZA
dc.description.abstractBACKGROUND:The induction of antigen-specific CD8+ T cell cytokine responses against an attenuated, oral recombinant Salmonella enterica serovar Typhimurium vaccine expressing a green fluorescent protein (GFP) model antigen was investigated. A GFP expression plasmid was constructed in which the gfp gene was fused in-frame with the 5' domain of the Escherichia coli beta-galactosidase alpha-gene fragment with expression under the lac promoter. Groups of mice were orally immunized three times with the bacteria and systemic CD8+ T cell cytokine responses were evaluated. RESULTS: High level of the GFP model antigen was expressed by the recombinant Salmonella vaccine vector. Systemic GFP-specific CD8+ T cell cytokine (IFN-gamma and IL-4) immune responses were detected after mice were orally vaccinated with the bacteria. It was shown that 226 net IFN-gamma and 132 net IL-4 GFP-specific SFUs/10e6 splenocytes were formed in an ELISPOT assay. The level of IFN-gamma produced by GFP peptide-stimulated cells was 65.2-fold above background (p < 0.05). The level of IL-4 produced by the cells was 10.4-fold above background (p < 0.05). CONCLUSION: These results suggested that a high expressing recombinant Salmonella vaccine given orally to mice would elicit antigen-specific CD8+ T cell responses in the spleen. Salmonella bacteria may, therefore, be used as potential mucosal vaccine vectors.en_ZA
dc.identifier.apacitationChin'ombe, N., Bourn, W., Williamson, A., & Shephard, E. (2009). An oral recombinant Salmonella enterica serovar Typhimurium mutant elicits systemic antigen-specific CD8+ T cell cytokine responses in mice. <i>Gut Pathogens</i>, http://hdl.handle.net/11427/14449en_ZA
dc.identifier.chicagocitationChin'ombe, Nyasha, William Bourn, Anna-Lise Williamson, and Enid Shephard "An oral recombinant Salmonella enterica serovar Typhimurium mutant elicits systemic antigen-specific CD8+ T cell cytokine responses in mice." <i>Gut Pathogens</i> (2009) http://hdl.handle.net/11427/14449en_ZA
dc.identifier.citationChin'ombe, N., Bourn, W. R., Williamson, A. L., & Shephard, E. G. (2009). An oral recombinant Salmonella enterica serovar Typhimurium mutant elicits systemic antigen-specific CD8+ T cell cytokine responses in mice. Gut pathogens, 1(1), 9.en_ZA
dc.identifier.ris TY - Journal Article AU - Chin'ombe, Nyasha AU - Bourn, William AU - Williamson, Anna-Lise AU - Shephard, Enid AB - BACKGROUND:The induction of antigen-specific CD8+ T cell cytokine responses against an attenuated, oral recombinant Salmonella enterica serovar Typhimurium vaccine expressing a green fluorescent protein (GFP) model antigen was investigated. A GFP expression plasmid was constructed in which the gfp gene was fused in-frame with the 5' domain of the Escherichia coli beta-galactosidase alpha-gene fragment with expression under the lac promoter. Groups of mice were orally immunized three times with the bacteria and systemic CD8+ T cell cytokine responses were evaluated. RESULTS: High level of the GFP model antigen was expressed by the recombinant Salmonella vaccine vector. Systemic GFP-specific CD8+ T cell cytokine (IFN-gamma and IL-4) immune responses were detected after mice were orally vaccinated with the bacteria. It was shown that 226 net IFN-gamma and 132 net IL-4 GFP-specific SFUs/10e6 splenocytes were formed in an ELISPOT assay. The level of IFN-gamma produced by GFP peptide-stimulated cells was 65.2-fold above background (p < 0.05). The level of IL-4 produced by the cells was 10.4-fold above background (p < 0.05). CONCLUSION: These results suggested that a high expressing recombinant Salmonella vaccine given orally to mice would elicit antigen-specific CD8+ T cell responses in the spleen. Salmonella bacteria may, therefore, be used as potential mucosal vaccine vectors. DA - 2009 DB - OpenUCT DO - 10.1186/1757-4749-1-9 DP - University of Cape Town J1 - Gut Pathogens LK - https://open.uct.ac.za PB - University of Cape Town PY - 2009 T1 - An oral recombinant Salmonella enterica serovar Typhimurium mutant elicits systemic antigen-specific CD8+ T cell cytokine responses in mice TI - An oral recombinant Salmonella enterica serovar Typhimurium mutant elicits systemic antigen-specific CD8+ T cell cytokine responses in mice UR - http://hdl.handle.net/11427/14449 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14449
dc.identifier.urihttp://dx.doi.org/10.1186/1757-4749-1-9
dc.identifier.vancouvercitationChin'ombe N, Bourn W, Williamson A, Shephard E. An oral recombinant Salmonella enterica serovar Typhimurium mutant elicits systemic antigen-specific CD8+ T cell cytokine responses in mice. Gut Pathogens. 2009; http://hdl.handle.net/11427/14449.en_ZA
dc.language.isoengen_ZA
dc.publisherBioMed Central Ltden_ZA
dc.publisher.departmentInstitute of Infectious Disease and Molecular Medicineen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.rights.holder2009 Chin'ombe et al; licensee BioMed Central Ltd.en_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_ZA
dc.sourceGut Pathogensen_ZA
dc.source.urihttp://www.gutpathogens.com/en_ZA
dc.subject.otherImmune responsesen_ZA
dc.subject.otherSalmonellaen_ZA
dc.titleAn oral recombinant Salmonella enterica serovar Typhimurium mutant elicits systemic antigen-specific CD8+ T cell cytokine responses in miceen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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