Nested clade analysis of geographic structure in the morphologically variable Themeda triandra in South Africa

dc.contributor.advisorBond, William Jen_ZA
dc.contributor.advisorHedderson, Terry Aen_ZA
dc.contributor.authorOatley, Graemeen_ZA
dc.date.accessioned2017-11-16T07:25:13Z
dc.date.available2017-11-16T07:25:13Z
dc.date.issued2004en_ZA
dc.date.updated2017-07-26T15:05:01Z
dc.description.abstractThe use of phylogeography in plant systems has been on the increase in recent years with the use of chloroplast DNA to detect sufficient intraspecific variation to reach significant conclusions about plant species histories, both temporally and spatially. In this study, the geographic structure and possible origin of the morphologically variable Themeda triandra is explored. The trnF - trnC and psbD - trnS gene regions of the cpDNA were used to find 12 haplotypes found in 11 populations of T. triandra that encompass the species large distributional range. A haplotype tree was constructed that showed the relationship of the 11 haplotypes (haplotype_H12 was excluded as it fell outside of the 95% confidence limit), with haplotype H6 inferred to be the ancestral haplotype. A nested clade analysis was performed with the results used to infer the geographic structure of T. triandra within South Africa. Significant results showed that there was restricted gene flow with nested clades involving the three Free State populations, indicating that there are barriers to gene flow with other haplotypes. The ancestral haplotype showed long distance colonisation, with a probable root of this colonisation being the Kruger National Park. This is the proposed point of introduction of T. triandra into South Africa, with results from this study supporting this proposal. A substantial amount of gene flow (25.49%; AMOV A) between populations is observed, with this probably being due to the widespread distribution of haplotypes H6 and H10. It is thought that T. triandra followed two migration routes within South Africa: one along the coast, with the other inland above the escarpment where populations became genetically isolated from populations below the escarpment. Further studies may look for a correlation between morphological variants of T. triandra and the cpDNA haplotypes found within the species.en_ZA
dc.identifier.apacitationOatley, G. (2004). <i>Nested clade analysis of geographic structure in the morphologically variable Themeda triandra in South Africa</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Biological Sciences. Retrieved from http://hdl.handle.net/11427/26314en_ZA
dc.identifier.chicagocitationOatley, Graeme. <i>"Nested clade analysis of geographic structure in the morphologically variable Themeda triandra in South Africa."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Biological Sciences, 2004. http://hdl.handle.net/11427/26314en_ZA
dc.identifier.citationOatley, G. 2004. Nested clade analysis of geographic structure in the morphologically variable Themeda triandra in South Africa. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Oatley, Graeme AB - The use of phylogeography in plant systems has been on the increase in recent years with the use of chloroplast DNA to detect sufficient intraspecific variation to reach significant conclusions about plant species histories, both temporally and spatially. In this study, the geographic structure and possible origin of the morphologically variable Themeda triandra is explored. The trnF - trnC and psbD - trnS gene regions of the cpDNA were used to find 12 haplotypes found in 11 populations of T. triandra that encompass the species large distributional range. A haplotype tree was constructed that showed the relationship of the 11 haplotypes (haplotype_H12 was excluded as it fell outside of the 95% confidence limit), with haplotype H6 inferred to be the ancestral haplotype. A nested clade analysis was performed with the results used to infer the geographic structure of T. triandra within South Africa. Significant results showed that there was restricted gene flow with nested clades involving the three Free State populations, indicating that there are barriers to gene flow with other haplotypes. The ancestral haplotype showed long distance colonisation, with a probable root of this colonisation being the Kruger National Park. This is the proposed point of introduction of T. triandra into South Africa, with results from this study supporting this proposal. A substantial amount of gene flow (25.49%; AMOV A) between populations is observed, with this probably being due to the widespread distribution of haplotypes H6 and H10. It is thought that T. triandra followed two migration routes within South Africa: one along the coast, with the other inland above the escarpment where populations became genetically isolated from populations below the escarpment. Further studies may look for a correlation between morphological variants of T. triandra and the cpDNA haplotypes found within the species. DA - 2004 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2004 T1 - Nested clade analysis of geographic structure in the morphologically variable Themeda triandra in South Africa TI - Nested clade analysis of geographic structure in the morphologically variable Themeda triandra in South Africa UR - http://hdl.handle.net/11427/26314 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/26314
dc.identifier.vancouvercitationOatley G. Nested clade analysis of geographic structure in the morphologically variable Themeda triandra in South Africa. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Biological Sciences, 2004 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/26314en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Biological Sciencesen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBotanyen_ZA
dc.subject.otherPlant Ecologyen_ZA
dc.subject.otherPhylogeographyen_ZA
dc.titleNested clade analysis of geographic structure in the morphologically variable Themeda triandra in South Africaen_ZA
dc.typeBachelor Thesis
dc.type.qualificationlevelHonours
dc.type.qualificationnameBSc (Hons)en_ZA
uct.type.filetype
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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