Protocol: Precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli

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dc.contributor.authorRoden, Lauraen_ZA
dc.contributor.authorGottgens, Bertholden_ZA
dc.contributor.authorMutasa-Gottgens, Effieen_ZA
dc.date.accessioned2015-10-12T10:57:58Z
dc.date.available2015-10-12T10:57:58Z
dc.date.issued2005en_ZA
dc.description.abstractPlant genome sequence data now provide opportunities to conduct molecular genetic studies at the level of the whole gene locus and above. Such studies will be greatly facilitated by adopting and developing further the new generation of genetic engineering tools, based on homologous recombination cloning in Escherichia coli, which are free from the constraints imposed by the availability of suitably positioned restriction sites. Here we describe the basis for homologous recombination cloning in E. coli, the available tools and resources, together with a protocol for long range cloning and manipulation of an Arabidopsis thaliana gene locus, to create constructs co-ordinately driven by locus-specific regulatory elements.en_ZA
dc.identifier.apacitationRoden, L., Gottgens, B., & Mutasa-Gottgens, E. (2005). Protocol: Precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli. <i>Plant Methods</i>, http://hdl.handle.net/11427/14196en_ZA
dc.identifier.chicagocitationRoden, Laura, Berthold Gottgens, and Effie Mutasa-Gottgens "Protocol: Precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli." <i>Plant Methods</i> (2005) http://hdl.handle.net/11427/14196en_ZA
dc.identifier.citationRoden, L. C., Göttgens, B., & Mutasa-Göttgens, E. S. (2005). Protocol: Precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli. Plant methods, 1(1), 1-12.en_ZA
dc.identifier.ris TY - Journal Article AU - Roden, Laura AU - Gottgens, Berthold AU - Mutasa-Gottgens, Effie AB - Plant genome sequence data now provide opportunities to conduct molecular genetic studies at the level of the whole gene locus and above. Such studies will be greatly facilitated by adopting and developing further the new generation of genetic engineering tools, based on homologous recombination cloning in Escherichia coli, which are free from the constraints imposed by the availability of suitably positioned restriction sites. Here we describe the basis for homologous recombination cloning in E. coli, the available tools and resources, together with a protocol for long range cloning and manipulation of an Arabidopsis thaliana gene locus, to create constructs co-ordinately driven by locus-specific regulatory elements. DA - 2005 DB - OpenUCT DO - 10.1186/1746-4811-1-6 DP - University of Cape Town J1 - Plant Methods LK - https://open.uct.ac.za PB - University of Cape Town PY - 2005 T1 - Protocol: Precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli TI - Protocol: Precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli UR - http://hdl.handle.net/11427/14196 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14196
dc.identifier.urihttp://dx.doi.org/10.1186/1746-4811-1-6
dc.identifier.vancouvercitationRoden L, Gottgens B, Mutasa-Gottgens E. Protocol: Precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli. Plant Methods. 2005; http://hdl.handle.net/11427/14196.en_ZA
dc.language.isoengen_ZA
dc.publisherBioMed Central Ltden_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Licenseen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en_ZA
dc.sourcePlant Methodsen_ZA
dc.source.urihttp://www.plantmethods.com/en_ZA
dc.subject.otherMolecular and Cell Biologyen_ZA
dc.titleProtocol: Precision engineering of plant gene loci by homologous recombination cloning in Escherichia colien_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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