Molecular mechanisms of recombination restriction in the envelope gene of the human immunodeficiency virus

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dc.contributor.authorSimon-Loriere, Etienneen_ZA
dc.contributor.authorGaletto, Romanen_ZA
dc.contributor.authorHamoudi, Meriemen_ZA
dc.contributor.authorArcher, Johnen_ZA
dc.contributor.authorLefeuvre, Pierreen_ZA
dc.contributor.authorMartin, Darren Pen_ZA
dc.contributor.authorRobertson, David Len_ZA
dc.contributor.authorNegroni, Matteoen_ZA
dc.date.accessioned2016-01-02T05:05:44Z
dc.date.available2016-01-02T05:05:44Z
dc.date.issued2009en_ZA
dc.description.abstractAuthor Summary Recombination allows mixing portions of genomes of different origins, generating chimeric genes and genomes. With respect to the random generation of new mutations, it can lead to the simultaneous insertion of several substitutions, introducing more drastic changes in the genome. Furthermore, recombination is expected to yield a higher proportion of functional products since it combines variants that already exist in the population and that are therefore compatible with the survival of the organism. However, when recombination involves genetically distant strains, it can be constrained by the necessity to retain the functionality of the resulting products. In pathogens, which are subjected to strong selective pressures, recombination is particularly important, and several viruses, such as the human immunodeficiency virus (HIV), readily recombine. Here, we demonstrate the existence of preferential regions for recombination in the HIV-1 envelope gene when crossing sequences representative of strains observed to recombine in vivo. Furthermore, some recombinants give a decreased proportion of functional products. When considering these factors, one can retrace the history of most natural HIV recombinants. Recombination in HIV appears not so unpredictable, therefore, and the existence of recombinants that frequently generate nonfunctional products highlights previously unappreciated limits of the genetic flexibility of HIV.en_ZA
dc.identifier.apacitationSimon-Loriere, E., Galetto, R., Hamoudi, M., Archer, J., Lefeuvre, P., Martin, D. P., ... Negroni, M. (2009). Molecular mechanisms of recombination restriction in the envelope gene of the human immunodeficiency virus. <i>PLoS One</i>, http://hdl.handle.net/11427/16157en_ZA
dc.identifier.chicagocitationSimon-Loriere, Etienne, Roman Galetto, Meriem Hamoudi, John Archer, Pierre Lefeuvre, Darren P Martin, David L Robertson, and Matteo Negroni "Molecular mechanisms of recombination restriction in the envelope gene of the human immunodeficiency virus." <i>PLoS One</i> (2009) http://hdl.handle.net/11427/16157en_ZA
dc.identifier.citationSimon-Loriere, E., Galetto, R., Hamoudi, M., Archer, J., Lefeuvre, P., Martin, D. P., ... & Negroni, M. (2009). Molecular mechanisms of recombination restriction in the envelope gene of the human immunodeficiency virus. PLoS Pathog, 5(5), e1000418. doi:10.1371/journal.ppat.1000418en_ZA
dc.identifier.ris TY - Journal Article AU - Simon-Loriere, Etienne AU - Galetto, Roman AU - Hamoudi, Meriem AU - Archer, John AU - Lefeuvre, Pierre AU - Martin, Darren P AU - Robertson, David L AU - Negroni, Matteo AB - Author Summary Recombination allows mixing portions of genomes of different origins, generating chimeric genes and genomes. With respect to the random generation of new mutations, it can lead to the simultaneous insertion of several substitutions, introducing more drastic changes in the genome. Furthermore, recombination is expected to yield a higher proportion of functional products since it combines variants that already exist in the population and that are therefore compatible with the survival of the organism. However, when recombination involves genetically distant strains, it can be constrained by the necessity to retain the functionality of the resulting products. In pathogens, which are subjected to strong selective pressures, recombination is particularly important, and several viruses, such as the human immunodeficiency virus (HIV), readily recombine. Here, we demonstrate the existence of preferential regions for recombination in the HIV-1 envelope gene when crossing sequences representative of strains observed to recombine in vivo. Furthermore, some recombinants give a decreased proportion of functional products. When considering these factors, one can retrace the history of most natural HIV recombinants. Recombination in HIV appears not so unpredictable, therefore, and the existence of recombinants that frequently generate nonfunctional products highlights previously unappreciated limits of the genetic flexibility of HIV. DA - 2009 DB - OpenUCT DO - 10.1371/journal.ppat.1000418 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2009 T1 - Molecular mechanisms of recombination restriction in the envelope gene of the human immunodeficiency virus TI - Molecular mechanisms of recombination restriction in the envelope gene of the human immunodeficiency virus UR - http://hdl.handle.net/11427/16157 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/16157
dc.identifier.urihttp://dx.doi.org/10.1371/journal.ppat.1000418
dc.identifier.vancouvercitationSimon-Loriere E, Galetto R, Hamoudi M, Archer J, Lefeuvre P, Martin DP, et al. Molecular mechanisms of recombination restriction in the envelope gene of the human immunodeficiency virus. PLoS One. 2009; http://hdl.handle.net/11427/16157.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentInstitute of Infectious Disease and Molecular Medicineen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2009 Simon-Loriere et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plospathogensen_ZA
dc.subject.otherRecombinant proteinsen_ZA
dc.subject.otherHIVen_ZA
dc.subject.otherDNA recombinationen_ZA
dc.subject.otherHIV-1en_ZA
dc.subject.otherSequence analysisen_ZA
dc.subject.otherDNA sequence analysisen_ZA
dc.subject.otherEvolutionary geneticsen_ZA
dc.subject.otherViral evolutionen_ZA
dc.titleMolecular mechanisms of recombination restriction in the envelope gene of the human immunodeficiency virusen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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