Species-specific DNA probes for the identification of Actinobacillus actinomycetemcomitans

Simple item page
dc.contributor.advisorSteyn, Lafras Men_ZA
dc.contributor.authorEmanuel, Margoten_ZA
dc.date.accessioned2017-08-23T13:12:39Z
dc.date.available2017-08-23T13:12:39Z
dc.date.issued1990en_ZA
dc.date.updated2017-07-10T12:42:58Z
dc.description.abstractA DNA probe was developed for the identification of the periodontal pathogen, Actinobacillus actinomycetemcomitans. Chromosomal DNA was extracted from A. actinomycetemcomitans, digested with a restriction enzyme, Sau3A, ligated to plasmid DNA (pUC18) and transformed into JM109 cells to give a partial A. actinomycetemcomitans library. The library was screened using Southern blot analysis. Out of the nine inserts tested, one was found to be species specific as it did not cross-hybridise to Haemophilus aphrophilus, a closely related organism which occurs in the normal oral microflora, nor did it cross-hybridise with 7 species of Bacteroides tested. A level of detection of 104 cells or 50ng of A. actinomycetemcomitans was obtained. The probe has a length of 779bp and out of 30 restriction enzymes tested, only SspI was found to have a restriction site in the insert. The probe was tested on clinical specimens obtained from five different periodontitis patient groups and was shown to correlate with culture results in eighteen out of twenty-two cases in detecting A. actinomycetemcomitans.en_ZA
dc.identifier.apacitationEmanuel, M. (1990). <i>Species-specific DNA probes for the identification of Actinobacillus actinomycetemcomitans</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Medical Microbiology. Retrieved from http://hdl.handle.net/11427/24965en_ZA
dc.identifier.chicagocitationEmanuel, Margot. <i>"Species-specific DNA probes for the identification of Actinobacillus actinomycetemcomitans."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Medical Microbiology, 1990. http://hdl.handle.net/11427/24965en_ZA
dc.identifier.citationEmanuel, M. 1990. Species-specific DNA probes for the identification of Actinobacillus actinomycetemcomitans. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Emanuel, Margot AB - A DNA probe was developed for the identification of the periodontal pathogen, Actinobacillus actinomycetemcomitans. Chromosomal DNA was extracted from A. actinomycetemcomitans, digested with a restriction enzyme, Sau3A, ligated to plasmid DNA (pUC18) and transformed into JM109 cells to give a partial A. actinomycetemcomitans library. The library was screened using Southern blot analysis. Out of the nine inserts tested, one was found to be species specific as it did not cross-hybridise to Haemophilus aphrophilus, a closely related organism which occurs in the normal oral microflora, nor did it cross-hybridise with 7 species of Bacteroides tested. A level of detection of 104 cells or 50ng of A. actinomycetemcomitans was obtained. The probe has a length of 779bp and out of 30 restriction enzymes tested, only SspI was found to have a restriction site in the insert. The probe was tested on clinical specimens obtained from five different periodontitis patient groups and was shown to correlate with culture results in eighteen out of twenty-two cases in detecting A. actinomycetemcomitans. DA - 1990 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1990 T1 - Species-specific DNA probes for the identification of Actinobacillus actinomycetemcomitans TI - Species-specific DNA probes for the identification of Actinobacillus actinomycetemcomitans UR - http://hdl.handle.net/11427/24965 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/24965
dc.identifier.vancouvercitationEmanuel M. Species-specific DNA probes for the identification of Actinobacillus actinomycetemcomitans. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Medical Microbiology, 1990 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/24965en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Medical Microbiologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherActinobacillus - pathogenicity~DNA Probes - analysis~Periodontitis - etiologyen_ZA
dc.titleSpecies-specific DNA probes for the identification of Actinobacillus actinomycetemcomitansen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetype
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Emanuel_Species_specific_DNA_probes_1992_1.pdf
Size:
6.68 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Masters