Inhibition of glyoxylate conversion to oxalate in cultured human cells by the carbonyl-scavenging drug, aminoguanidine

dc.contributor.authorBerman, P
dc.contributor.authorVan der Watt, F
dc.contributor.authorHack, D J
dc.contributor.authorBaumgarten, I
dc.date.accessioned2017-04-10T08:07:00Z
dc.date.available2017-04-10T08:07:00Z
dc.date.issued2005
dc.date.updated2016-01-05T09:54:04Z
dc.description.abstractCalcium oxalate is the most frequent cause of kidney stones, and is responsible for the damage to kidneys and other organs observed in inherited disorders of oxalate metabolism. Most oxalate produced in the body is derived from its metabolic precursor, glyoxylate. Thus, any means of scavenging glyoxylate to a non-toxic product, thereby diverting it away from oxalate synthesis, has considerable therapeutic implications. Here we show that aminoguanidine, a compound with a proven safety record and used for many years to prevent long-term complications of diabetes, binds glyoxylate covalently and reduces its conversion to oxalate by human liver- and lymphocyte-derived cell lines by >90%. We propose that scavenging glyoxylate with aminoguanidine or its congeners may provide a means of reducing oxalate production in vivo, and advocate the tissue culture system described here as a convenient means for testing such agents in vitro. A serendipitous finding to emerge from our study was the abiotic and strongly pH-dependent formation of oxalate from ascorbate, which has implications for the contribution of ascorbate to urine oxalate excretion.
dc.identifier.apacitationBerman, P., Van der Watt, F., Hack, D. J., & Baumgarten, I. (2005). Inhibition of glyoxylate conversion to oxalate in cultured human cells by the carbonyl-scavenging drug, aminoguanidine. <i>South African Journal of Science</i>, http://hdl.handle.net/11427/24171en_ZA
dc.identifier.chicagocitationBerman, P, F Van der Watt, D J Hack, and I Baumgarten "Inhibition of glyoxylate conversion to oxalate in cultured human cells by the carbonyl-scavenging drug, aminoguanidine." <i>South African Journal of Science</i> (2005) http://hdl.handle.net/11427/24171en_ZA
dc.identifier.citationBerman, P. A., Van der Watt, G. F., Hack, D. J., & Baumgarten, I. (2005). Inhibition of glyoxylate conversion to oxalate in cultured human cells by the carbonyl-scavenging drug, aminoguanidine: research letter. South African journal of science, 101(5 & 6), p-249.
dc.identifier.ris TY - Journal Article AU - Berman, P AU - Van der Watt, F AU - Hack, D J AU - Baumgarten, I AB - Calcium oxalate is the most frequent cause of kidney stones, and is responsible for the damage to kidneys and other organs observed in inherited disorders of oxalate metabolism. Most oxalate produced in the body is derived from its metabolic precursor, glyoxylate. Thus, any means of scavenging glyoxylate to a non-toxic product, thereby diverting it away from oxalate synthesis, has considerable therapeutic implications. Here we show that aminoguanidine, a compound with a proven safety record and used for many years to prevent long-term complications of diabetes, binds glyoxylate covalently and reduces its conversion to oxalate by human liver- and lymphocyte-derived cell lines by >90%. We propose that scavenging glyoxylate with aminoguanidine or its congeners may provide a means of reducing oxalate production in vivo, and advocate the tissue culture system described here as a convenient means for testing such agents in vitro. A serendipitous finding to emerge from our study was the abiotic and strongly pH-dependent formation of oxalate from ascorbate, which has implications for the contribution of ascorbate to urine oxalate excretion. DA - 2005 DB - OpenUCT DP - University of Cape Town J1 - South African Journal of Science LK - https://open.uct.ac.za PB - University of Cape Town PY - 2005 T1 - Inhibition of glyoxylate conversion to oxalate in cultured human cells by the carbonyl-scavenging drug, aminoguanidine TI - Inhibition of glyoxylate conversion to oxalate in cultured human cells by the carbonyl-scavenging drug, aminoguanidine UR - http://hdl.handle.net/11427/24171 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/24171
dc.identifier.vancouvercitationBerman P, Van der Watt F, Hack DJ, Baumgarten I. Inhibition of glyoxylate conversion to oxalate in cultured human cells by the carbonyl-scavenging drug, aminoguanidine. South African Journal of Science. 2005; http://hdl.handle.net/11427/24171.en_ZA
dc.language.isoeng
dc.publisher.departmentDivision of Chemical Pathologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceSouth African Journal of Science
dc.source.urihttp://www.sajs.co.za/
dc.titleInhibition of glyoxylate conversion to oxalate in cultured human cells by the carbonyl-scavenging drug, aminoguanidine
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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