Transient expression of Human papillomavirus type 16 L1 protein in Nicotiana benthamiana using an infectious tobamovirus vector

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dc.contributor.authorVarsani, Arvind
dc.contributor.authorWilliamson, Anna-Lise
dc.contributor.authorStewart, Debbie
dc.contributor.authorRybicki, Edward P
dc.date.accessioned2016-07-26T08:41:32Z
dc.date.available2016-07-26T08:41:32Z
dc.date.issued2006
dc.date.updated2016-07-15T15:11:46Z
dc.description.abstractA Tobacco mosaic virus (TMV)-derived vector was used to express a native Human papillomavirus type 16 (HPV-16) L1 gene in Nicotiana benthamiana by means of infectious in vitro RNA transcripts inoculated onto N. benthamiana plants. HPV-16 L1 protein expression was quantitated by enzyme-linked immunosorbent assays (ELISA) after concentration of the plant extract. We estimated that the L1 product yield was 20–37 g/kg of fresh leaf material. The L1 protein in the concentrated extract was antigenically characterised using the neutralising and conformation-specific Mabs H16:V5 and H16:E70, which bound to the plant-produced protein. Particles observed by transmission electron microscopy were mainly capsomers but virus-like particles (VLPs) similar to those produced in other systems were also present. Immunisation of rabbits with the concentrated plant extract induced a weak immune response. This is the first report of the successful expression of an HPV L1 gene in plants using a plant virus vector.en_ZA
dc.identifierhttp://dx.doi.org/10.1016/j.virusres.2006.01.022
dc.identifier.apacitationVarsani, A., Williamson, A., Stewart, D., & Rybicki, E. P. (2006). Transient expression of Human papillomavirus type 16 L1 protein in Nicotiana benthamiana using an infectious tobamovirus vector. <i>Virus Research</i>, http://hdl.handle.net/11427/20741en_ZA
dc.identifier.chicagocitationVarsani, Arvind, Anna-Lise Williamson, Debbie Stewart, and Edward P Rybicki "Transient expression of Human papillomavirus type 16 L1 protein in Nicotiana benthamiana using an infectious tobamovirus vector." <i>Virus Research</i> (2006) http://hdl.handle.net/11427/20741en_ZA
dc.identifier.citationVarsani, A., Williamson, A. L., Stewart, D., & Rybicki, E. P. (2006). Transient expression of Human papillomavirus type 16 L1 protein in Nicotiana benthamiana using an infectious tobamovirus vector. Virus research, 120(1), 91-96.en_ZA
dc.identifier.issn0168-1702en_ZA
dc.identifier.ris TY - Journal Article AU - Varsani, Arvind AU - Williamson, Anna-Lise AU - Stewart, Debbie AU - Rybicki, Edward P AB - A Tobacco mosaic virus (TMV)-derived vector was used to express a native Human papillomavirus type 16 (HPV-16) L1 gene in Nicotiana benthamiana by means of infectious in vitro RNA transcripts inoculated onto N. benthamiana plants. HPV-16 L1 protein expression was quantitated by enzyme-linked immunosorbent assays (ELISA) after concentration of the plant extract. We estimated that the L1 product yield was 20–37 g/kg of fresh leaf material. The L1 protein in the concentrated extract was antigenically characterised using the neutralising and conformation-specific Mabs H16:V5 and H16:E70, which bound to the plant-produced protein. Particles observed by transmission electron microscopy were mainly capsomers but virus-like particles (VLPs) similar to those produced in other systems were also present. Immunisation of rabbits with the concentrated plant extract induced a weak immune response. This is the first report of the successful expression of an HPV L1 gene in plants using a plant virus vector. DA - 2006 DB - OpenUCT DP - University of Cape Town J1 - Virus Research LK - https://open.uct.ac.za PB - University of Cape Town PY - 2006 SM - 0168-1702 T1 - Transient expression of Human papillomavirus type 16 L1 protein in Nicotiana benthamiana using an infectious tobamovirus vector TI - Transient expression of Human papillomavirus type 16 L1 protein in Nicotiana benthamiana using an infectious tobamovirus vector UR - http://hdl.handle.net/11427/20741 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20741
dc.identifier.vancouvercitationVarsani A, Williamson A, Stewart D, Rybicki EP. Transient expression of Human papillomavirus type 16 L1 protein in Nicotiana benthamiana using an infectious tobamovirus vector. Virus Research. 2006; http://hdl.handle.net/11427/20741.en_ZA
dc.language.isoeng
dc.publisherElsevieren_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)*
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en_ZA
dc.sourceVirus Researchen_ZA
dc.source.urihttp://www.sciencedirect.com/science/journal/01681702
dc.subject.otherPapillomavirus
dc.subject.otherHPV-16
dc.subject.otherVLP
dc.subject.otherVaccine
dc.subject.otherPlant expression
dc.subject.otherTMV vector
dc.titleTransient expression of Human papillomavirus type 16 L1 protein in Nicotiana benthamiana using an infectious tobamovirus vectoren_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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