Altered ratio of IFN-γ/IL-10 in patients with drug resistant Mycobacterium tuberculosis and HIV-tuberculosis immune reconstitution inflammatory syndrome

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dc.contributor.authorSkolimowska, Keira Hen_ZA
dc.contributor.authorRangaka, Molebogeng Xen_ZA
dc.contributor.authorMeintjes, Graemeen_ZA
dc.contributor.authorPepper, Dominique Jen_ZA
dc.contributor.authorSeldon, Ronnetten_ZA
dc.contributor.authorMatthews, Kerrynen_ZA
dc.contributor.authorWilkinson, Robert Jen_ZA
dc.contributor.authorWilkinson, Katalin Aen_ZA
dc.date.accessioned2015-12-28T06:47:43Z
dc.date.available2015-12-28T06:47:43Z
dc.date.issued2012en_ZA
dc.description.abstractWe have described a clinical relationship between HIV-Tuberculosis Immune Reconstitution Inflammatory Syndrome (TB-IRIS) and anti-tubercular drug resistance. Here we studied the immune response of TB-IRIS patients from whom a drug-resistant (n = 11) or drug-susceptible (n = 25) Mycobacterium tuberculosis (MTB) strain was isolated after presenting with TB-IRIS. ELISpot analysis and multiplex cytokine analysis of the supernatant collected from peripheral blood mononuclear cells stimulated overnight with the heat-killed H37Rv MTB laboratory strain was used. Although there was no statistical difference in IFN-gamma ELISpot responses between the two groups, the results point towards higher bacterial load in the drug-resistant patients, possibly due to failed therapy. The ratio between secreted IFN-gamma/IL-10 and IL-2/IL-10 was significantly lower in TB-IRIS patients in whom the cause of TB was a drug-resistant strain compared to those with a fully sensitive strain (p = 0.02). Since host immune responses are dependent on the bacterial load, we hypothesise that the impaired cytokine balance is likely to be caused by the poorly controlled bacterial growth in these patients.en_ZA
dc.identifier.apacitationSkolimowska, K. H., Rangaka, M. X., Meintjes, G., Pepper, D. J., Seldon, R., Matthews, K., ... Wilkinson, K. A. (2012). Altered ratio of IFN-γ/IL-10 in patients with drug resistant Mycobacterium tuberculosis and HIV-tuberculosis immune reconstitution inflammatory syndrome. <i>PLoS One</i>, http://hdl.handle.net/11427/16043en_ZA
dc.identifier.chicagocitationSkolimowska, Keira H, Molebogeng X Rangaka, Graeme Meintjes, Dominique J Pepper, Ronnett Seldon, Kerryn Matthews, Robert J Wilkinson, and Katalin A Wilkinson "Altered ratio of IFN-γ/IL-10 in patients with drug resistant Mycobacterium tuberculosis and HIV-tuberculosis immune reconstitution inflammatory syndrome." <i>PLoS One</i> (2012) http://hdl.handle.net/11427/16043en_ZA
dc.identifier.citationSkolimowska, K. H., Rangaka, M. X., Meintjes, G., Pepper, D. J., Seldon, R., Matthews, K., ... & Wilkinson, K. A. (2012). Altered ratio of IFN-γ/IL-10 in patients with drug resistant Mycobacterium tuberculosis and HIV-tuberculosis immune reconstitution inflammatory syndrome. PLoS ONE, 7(10), e46481. doi:10.1371/journal.pone.0046481en_ZA
dc.identifier.ris TY - Journal Article AU - Skolimowska, Keira H AU - Rangaka, Molebogeng X AU - Meintjes, Graeme AU - Pepper, Dominique J AU - Seldon, Ronnett AU - Matthews, Kerryn AU - Wilkinson, Robert J AU - Wilkinson, Katalin A AB - We have described a clinical relationship between HIV-Tuberculosis Immune Reconstitution Inflammatory Syndrome (TB-IRIS) and anti-tubercular drug resistance. Here we studied the immune response of TB-IRIS patients from whom a drug-resistant (n = 11) or drug-susceptible (n = 25) Mycobacterium tuberculosis (MTB) strain was isolated after presenting with TB-IRIS. ELISpot analysis and multiplex cytokine analysis of the supernatant collected from peripheral blood mononuclear cells stimulated overnight with the heat-killed H37Rv MTB laboratory strain was used. Although there was no statistical difference in IFN-gamma ELISpot responses between the two groups, the results point towards higher bacterial load in the drug-resistant patients, possibly due to failed therapy. The ratio between secreted IFN-gamma/IL-10 and IL-2/IL-10 was significantly lower in TB-IRIS patients in whom the cause of TB was a drug-resistant strain compared to those with a fully sensitive strain (p = 0.02). Since host immune responses are dependent on the bacterial load, we hypothesise that the impaired cytokine balance is likely to be caused by the poorly controlled bacterial growth in these patients. DA - 2012 DB - OpenUCT DO - 10.1371/journal.pone.0046481 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 T1 - Altered ratio of IFN-γ/IL-10 in patients with drug resistant Mycobacterium tuberculosis and HIV-tuberculosis immune reconstitution inflammatory syndrome TI - Altered ratio of IFN-γ/IL-10 in patients with drug resistant Mycobacterium tuberculosis and HIV-tuberculosis immune reconstitution inflammatory syndrome UR - http://hdl.handle.net/11427/16043 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/16043
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0046481
dc.identifier.vancouvercitationSkolimowska KH, Rangaka MX, Meintjes G, Pepper DJ, Seldon R, Matthews K, et al. Altered ratio of IFN-γ/IL-10 in patients with drug resistant Mycobacterium tuberculosis and HIV-tuberculosis immune reconstitution inflammatory syndrome. PLoS One. 2012; http://hdl.handle.net/11427/16043.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentInstitute of Infectious Disease and Molecular Medicineen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© Skolimowska et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plosoneen_ZA
dc.subject.otherMycobacterium tuberculosisen_ZA
dc.subject.otherTuberculosisen_ZA
dc.subject.otherCytokinesen_ZA
dc.subject.otherExtensively drug-resistant tuberculosisen_ZA
dc.subject.otherMulti-drug-resistant tuberculosisen_ZA
dc.subject.otherImmune responseen_ZA
dc.subject.otherEnzyme-linked immunoassaysen_ZA
dc.subject.otherAntiretroviral therapyen_ZA
dc.titleAltered ratio of IFN-γ/IL-10 in patients with drug resistant Mycobacterium tuberculosis and HIV-tuberculosis immune reconstitution inflammatory syndromeen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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