Helical structure of unidirectionally shadowed metal replicas of cyanide hydratase from Gloeocercospora sorghi.

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dc.contributor.authorWoodward, J D
dc.contributor.authorWeber, B W
dc.contributor.authorScheffer, M P
dc.contributor.authorBenedik, M J
dc.contributor.authorHoenger A
dc.contributor.authorSewell, B T
dc.date.accessioned2016-07-21T15:20:33Z
dc.date.available2016-07-21T15:20:33Z
dc.date.issued2008
dc.date.updated2016-07-21T15:19:09Z
dc.description.abstractThe helical filaments of the cyanide hydratase from Gloeocercospora sorghi have been reconstructed in three dimensions from freeze dried, unidirectionally shadowed specimens using iterative real-space helical reconstruction. The average power spectrum of all selected images has three clear reflections on different layer lines. The reconstruction is complicated by the fact that three possible indexing schemes are possible and reconstructions using the starting symmetries based on each of these indexing schemes converge on threedimensional volumes which appear plausible. Because only one side is visible in shadowed specimens, it is necessary to examine the phases from a single filament by cryo-electron microscopy in order to make an unequivocal assignment of the symmetry. Because of the novel nature of the reconstruction method used here, conventional cryo-EM methods were also used to determine a second reconstruction, allowing us to make comparisons between the two. The filament is shown to have a left-handed one-start helix with D1 symmetry, 5.46 dimers per turn and a pitch of 7.15 nm. The reconstruction suggests the presence of an interaction across the groove not previously seen in nitrilase helical fibres.en_ZA
dc.identifierhttp://dx.doi.org/10.1016/j.jsb.2007.09.019
dc.identifier.apacitationWoodward, J. D., Weber, B. W., Scheffer, M. P., Benedik, M. J., , & Sewell, B. T. (2008). Helical structure of unidirectionally shadowed metal replicas of cyanide hydratase from Gloeocercospora sorghi. <i>Journal of Structural Biology</i>, http://hdl.handle.net/11427/20589en_ZA
dc.identifier.chicagocitationWoodward, J D, B W Weber, M P Scheffer, M J Benedik, , and B T Sewell "Helical structure of unidirectionally shadowed metal replicas of cyanide hydratase from Gloeocercospora sorghi." <i>Journal of Structural Biology</i> (2008) http://hdl.handle.net/11427/20589en_ZA
dc.identifier.citationWoodward, J. D., Weber, B. W., Scheffer, M. P., Benedik, M. J., Hoenger, A., & Sewell, B. T. (2008). Helical structure of unidirectionally shadowed metal replicas of cyanide hydratase from Gloeocercospora sorghi. Journal of structural biology, 161(2), 111-119.en_ZA
dc.identifier.issn1047-8477en_ZA
dc.identifier.ris TY - Journal Article AU - Woodward, J D AU - Weber, B W AU - Scheffer, M P AU - Benedik, M J AU - Hoenger A AU - Sewell, B T AB - The helical filaments of the cyanide hydratase from Gloeocercospora sorghi have been reconstructed in three dimensions from freeze dried, unidirectionally shadowed specimens using iterative real-space helical reconstruction. The average power spectrum of all selected images has three clear reflections on different layer lines. The reconstruction is complicated by the fact that three possible indexing schemes are possible and reconstructions using the starting symmetries based on each of these indexing schemes converge on threedimensional volumes which appear plausible. Because only one side is visible in shadowed specimens, it is necessary to examine the phases from a single filament by cryo-electron microscopy in order to make an unequivocal assignment of the symmetry. Because of the novel nature of the reconstruction method used here, conventional cryo-EM methods were also used to determine a second reconstruction, allowing us to make comparisons between the two. The filament is shown to have a left-handed one-start helix with D1 symmetry, 5.46 dimers per turn and a pitch of 7.15 nm. The reconstruction suggests the presence of an interaction across the groove not previously seen in nitrilase helical fibres. DA - 2008 DB - OpenUCT DP - University of Cape Town J1 - Journal of Structural Biology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 SM - 1047-8477 T1 - Helical structure of unidirectionally shadowed metal replicas of cyanide hydratase from Gloeocercospora sorghi TI - Helical structure of unidirectionally shadowed metal replicas of cyanide hydratase from Gloeocercospora sorghi UR - http://hdl.handle.net/11427/20589 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20589
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S1047847707002377
dc.identifier.vancouvercitationWoodward JD, Weber BW, Scheffer MP, Benedik MJ, , Sewell BT. Helical structure of unidirectionally shadowed metal replicas of cyanide hydratase from Gloeocercospora sorghi. Journal of Structural Biology. 2008; http://hdl.handle.net/11427/20589.en_ZA
dc.languageengen_ZA
dc.publisherElsevieren_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceJournal of Structural Biologyen_ZA
dc.source.urihttp://www.journals.elsevier.com/journal-of-structural-biology/
dc.subject.otherCyanide hydratase
dc.subject.otherGloeocercospora sorghi
dc.subject.otherNitrilase
dc.subject.otherHelical reconstruction
dc.subject.otherShadowing
dc.subject.otherMidilab
dc.subject.otherIH
dc.titleHelical structure of unidirectionally shadowed metal replicas of cyanide hydratase from Gloeocercospora sorghi.en_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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