Mimicking filtration and transport of rotavirus and adenovirus in sand media using DNA-labeled, protein-coated silica nanoparticles

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dc.contributor.authorPang, Liping
dc.contributor.authorFarkas, Kata
dc.contributor.authorBennett, Grant
dc.contributor.authorVarsani, Arvind
dc.contributor.authorEasingwood, Richard
dc.contributor.authorTilley, Richard
dc.contributor.authorNowostawska, Urszula
dc.contributor.authorLin, Susan
dc.date.accessioned2018-03-26T11:54:59Z
dc.date.available2018-03-26T11:54:59Z
dc.date.issued2014
dc.date.updated2016-01-20T13:00:13Z
dc.description.abstractAbstract Rotavirus (RoV) and adenovirus (AdV) are important viral pathogens for the risk analysis of drinking water. Despite this, little is known about their retention and transport behaviors in porous media due to a lack of representative surrogates. We developed RoV and AdV surrogates by covalently coupling 70-nm sized silica nanoparticles with specific proteins and a DNA marker for sensitive detection. Filtration experiments using beach sand columns demonstrated the similarity of the surrogates' concentrations, filtration efficiencies and attachment kinetics to those of the target viruses. The surrogates showed the same magnitude of concentration reduction as the viruses. Conversely, MS2 phage (a traditional virus model) over-predicted concentrations of AdV and RoV by 1- and 2-orders of magnitude respectively. The surrogates remained stable in size, surface charge and DNA concentration for at least one year. They can be easily and rapidly detected down to a single particle. Preliminary tests suggest that they were readily detectable in a number of environmental waters and treated effluent. With up-scaling validation in pilot trials, the surrogates developed here could be a cost-effective new tool for studying virus retention and transport in porous media. Examples include assessing filter efficacy in water and wastewater treatment, tracking virus migration in groundwater after effluent land disposal, and establishing safe setback distances for groundwater protection.
dc.identifierhttp://dx.doi.org/10.1016/j.watres.2014.05.055
dc.identifier.apacitationPang, L., Farkas, K., Bennett, G., Varsani, A., Easingwood, R., Tilley, R., ... Lin, S. (2014). Mimicking filtration and transport of rotavirus and adenovirus in sand media using DNA-labeled, protein-coated silica nanoparticles. <i>Water Research</i>, http://hdl.handle.net/11427/27719en_ZA
dc.identifier.chicagocitationPang, Liping, Kata Farkas, Grant Bennett, Arvind Varsani, Richard Easingwood, Richard Tilley, Urszula Nowostawska, and Susan Lin "Mimicking filtration and transport of rotavirus and adenovirus in sand media using DNA-labeled, protein-coated silica nanoparticles." <i>Water Research</i> (2014) http://hdl.handle.net/11427/27719en_ZA
dc.identifier.citationPang, L., Farkas, K., Bennett, G., Varsani, A., Easingwood, R., Tilley, R., . . . Lin, S. (2014). Mimicking filtration and transport of rotavirus and adenovirus in sand media using DNA-labeled, protein-coated silica nanoparticles. Water Research, 62, 167-179. doi:10.1016/j.watres.2014.05.055
dc.identifier.ris TY - Journal Article AU - Pang, Liping AU - Farkas, Kata AU - Bennett, Grant AU - Varsani, Arvind AU - Easingwood, Richard AU - Tilley, Richard AU - Nowostawska, Urszula AU - Lin, Susan AB - Abstract Rotavirus (RoV) and adenovirus (AdV) are important viral pathogens for the risk analysis of drinking water. Despite this, little is known about their retention and transport behaviors in porous media due to a lack of representative surrogates. We developed RoV and AdV surrogates by covalently coupling 70-nm sized silica nanoparticles with specific proteins and a DNA marker for sensitive detection. Filtration experiments using beach sand columns demonstrated the similarity of the surrogates' concentrations, filtration efficiencies and attachment kinetics to those of the target viruses. The surrogates showed the same magnitude of concentration reduction as the viruses. Conversely, MS2 phage (a traditional virus model) over-predicted concentrations of AdV and RoV by 1- and 2-orders of magnitude respectively. The surrogates remained stable in size, surface charge and DNA concentration for at least one year. They can be easily and rapidly detected down to a single particle. Preliminary tests suggest that they were readily detectable in a number of environmental waters and treated effluent. With up-scaling validation in pilot trials, the surrogates developed here could be a cost-effective new tool for studying virus retention and transport in porous media. Examples include assessing filter efficacy in water and wastewater treatment, tracking virus migration in groundwater after effluent land disposal, and establishing safe setback distances for groundwater protection. DA - 2014 DB - OpenUCT DP - University of Cape Town J1 - Water Research LK - https://open.uct.ac.za PB - University of Cape Town PY - 2014 T1 - Mimicking filtration and transport of rotavirus and adenovirus in sand media using DNA-labeled, protein-coated silica nanoparticles TI - Mimicking filtration and transport of rotavirus and adenovirus in sand media using DNA-labeled, protein-coated silica nanoparticles UR - http://hdl.handle.net/11427/27719 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/27719
dc.identifier.vancouvercitationPang L, Farkas K, Bennett G, Varsani A, Easingwood R, Tilley R, et al. Mimicking filtration and transport of rotavirus and adenovirus in sand media using DNA-labeled, protein-coated silica nanoparticles. Water Research. 2014; http://hdl.handle.net/11427/27719.en_ZA
dc.language.isoeng
dc.publisher.departmentDivision of Medical Biochemistryen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceWater Research
dc.source.urihttp://www.journals.elsevier.com/water-research
dc.subject.otherVirus surrogates
dc.subject.otherNanoparticles
dc.subject.otherSand filtration
dc.subject.otherGroundwater
dc.subject.otherDNA-labeling
dc.subject.otherPorous media
dc.titleMimicking filtration and transport of rotavirus and adenovirus in sand media using DNA-labeled, protein-coated silica nanoparticles
dc.typeJournal Article
uct.type.filetypeText
uct.type.filetypeImage
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