Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor

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dc.contributor.authorMa, Pei-yinen_ZA
dc.date.accessioned2014-11-05T17:31:18Z
dc.date.available2014-11-05T17:31:18Z
dc.date.issued1996en_ZA
dc.descriptionBibliography: leaves 116-120.en_ZA
dc.description.abstractWound-inducible proteinase inhibitors in plants elicit a defence mechanism by inactivating the proteinases of insects. This triggers a feedback mechanism causing overproduction of digestive enzymes together with a decrease in appetite, leading to starvation. System in, a polypeptide proteinase inhibitor-inducing factor, when applied to cut stems of young tomato plants induces the accumulation of inhibitors in a manner similar to the normal wounding response. We designed and synthesised the minus strand oligonucleotide template complementary to the system in DNA sequence using Escherichia coli codon preferences. The double stranded fragment encoding the 18 amino acid residue systemin was cloned into pUCJ 8 for amplification and subcloning into pMAL-pk for expression as a maltose binding-fusion protein. The recombinant systemin was released by enterokinase and isolated by HPLC. After further purification, the physical characteristics including amino acid composition, peptide sequence and molecular weight of r-systemin were determined. When the recombinant peptide was applied to young tomato plants, it induced the accumulation of proteinase inhibitor I messenger RNA.en_ZA
dc.identifier.apacitationMa, P. (1996). <i>Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/9241en_ZA
dc.identifier.chicagocitationMa, Pei-yin. <i>"Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1996. http://hdl.handle.net/11427/9241en_ZA
dc.identifier.citationMa, P. 1996. Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Ma, Pei-yin AB - Wound-inducible proteinase inhibitors in plants elicit a defence mechanism by inactivating the proteinases of insects. This triggers a feedback mechanism causing overproduction of digestive enzymes together with a decrease in appetite, leading to starvation. System in, a polypeptide proteinase inhibitor-inducing factor, when applied to cut stems of young tomato plants induces the accumulation of inhibitors in a manner similar to the normal wounding response. We designed and synthesised the minus strand oligonucleotide template complementary to the system in DNA sequence using Escherichia coli codon preferences. The double stranded fragment encoding the 18 amino acid residue systemin was cloned into pUCJ 8 for amplification and subcloning into pMAL-pk for expression as a maltose binding-fusion protein. The recombinant systemin was released by enterokinase and isolated by HPLC. After further purification, the physical characteristics including amino acid composition, peptide sequence and molecular weight of r-systemin were determined. When the recombinant peptide was applied to young tomato plants, it induced the accumulation of proteinase inhibitor I messenger RNA. DA - 1996 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1996 T1 - Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor TI - Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor UR - http://hdl.handle.net/11427/9241 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/9241
dc.identifier.vancouvercitationMa P. Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1996 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/9241en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBiochemistryen_ZA
dc.titleChemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factoren_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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