Anomalies in the growth kinetics of Saccharomyces cerevisiae strains in aerobic chemostat cultures
| dc.contributor.author | De Kock, S H | |
| dc.contributor.author | Du Preez, J C | |
| dc.contributor.author | Kilian, S G | |
| dc.date.accessioned | 2016-08-17T09:22:52Z | |
| dc.date.available | 2016-08-17T09:22:52Z | |
| dc.date.issued | 2000 | |
| dc.date.updated | 2016-08-16T11:56:15Z | |
| dc.description.abstract | Aerobic glucose-limited chemostat cultivations were conducted with Saccharomyces cerevisiae strains NRRL Y132, ATCC 4126 and CBS 8066, using a complex medium. At low dilution rates all three strains utilised glucose oxidatively with high biomass yield coefficients, no ethanol production and very low steady-state residual glucose concentrations in the culture. Above a threshold dilution rate, respiro-fermentative (oxido-reductive) metabolism commenced, with simultaneous respiration and fermentation occurring, which is typical of Crabtree-positive yeasts. However, at high dilution rates the three strains responded differently. At high dilution rates S. cerevisiae CBS 8066 produced 7–8 g ethanol L−1 from 20 g glucose L−1 with concomitant low levels of residual glucose, which increased markedly only close to the wash-out dilution rate. By contrast, in the respiro-fermentative region both S. cerevisiae ATCC 4126 and NRRL Y132 produced much lower levels of ethanol (3–4 g L−1) than S. cerevisiae CBS 8066, concomitant with very high residual sugar concentrations, which was a significant deviation from Monod kinetics and appeared to be associated either with high growth rates or with a fermentative (or respiro-fermentative) metabolism. Supplementation of the cultures with inorganic or organic nutrients failed to improve ethanol production or glucose assimilation. | en_ZA |
| dc.identifier | http://dx.doi.org/10.1038/sj.jim.2900813 | |
| dc.identifier.apacitation | De Kock, S. H., Du Preez, J. C., & Kilian, S. G. (2000). Anomalies in the growth kinetics of Saccharomyces cerevisiae strains in aerobic chemostat cultures. <i> J Indust Biotechnol 24: 231-236</i>, http://hdl.handle.net/11427/21286 | en_ZA |
| dc.identifier.chicagocitation | De Kock, S H, J C Du Preez, and S G Kilian "Anomalies in the growth kinetics of Saccharomyces cerevisiae strains in aerobic chemostat cultures." <i> J Indust Biotechnol 24: 231-236</i> (2000) http://hdl.handle.net/11427/21286 | en_ZA |
| dc.identifier.citation | De Kock, S. H., Du Preez, J. C., & Kilian, S. G. (2000). Anomalies in the growth kinetics of Saccharomyces cerevisiae strains in aerobic chemostat cultures. Journal of Industrial Microbiology and Biotechnology, 24(4), 231-236. | en_ZA |
| dc.identifier.issn | 1367-5435 | en_ZA |
| dc.identifier.ris | TY - Journal Article AU - De Kock, S H AU - Du Preez, J C AU - Kilian, S G AB - Aerobic glucose-limited chemostat cultivations were conducted with Saccharomyces cerevisiae strains NRRL Y132, ATCC 4126 and CBS 8066, using a complex medium. At low dilution rates all three strains utilised glucose oxidatively with high biomass yield coefficients, no ethanol production and very low steady-state residual glucose concentrations in the culture. Above a threshold dilution rate, respiro-fermentative (oxido-reductive) metabolism commenced, with simultaneous respiration and fermentation occurring, which is typical of Crabtree-positive yeasts. However, at high dilution rates the three strains responded differently. At high dilution rates S. cerevisiae CBS 8066 produced 7–8 g ethanol L−1 from 20 g glucose L−1 with concomitant low levels of residual glucose, which increased markedly only close to the wash-out dilution rate. By contrast, in the respiro-fermentative region both S. cerevisiae ATCC 4126 and NRRL Y132 produced much lower levels of ethanol (3–4 g L−1) than S. cerevisiae CBS 8066, concomitant with very high residual sugar concentrations, which was a significant deviation from Monod kinetics and appeared to be associated either with high growth rates or with a fermentative (or respiro-fermentative) metabolism. Supplementation of the cultures with inorganic or organic nutrients failed to improve ethanol production or glucose assimilation. DA - 2000 DB - OpenUCT DP - University of Cape Town J1 - J Indust Biotechnol 24: 231-236 LK - https://open.uct.ac.za PB - University of Cape Town PY - 2000 SM - 1367-5435 T1 - Anomalies in the growth kinetics of Saccharomyces cerevisiae strains in aerobic chemostat cultures TI - Anomalies in the growth kinetics of Saccharomyces cerevisiae strains in aerobic chemostat cultures UR - http://hdl.handle.net/11427/21286 ER - | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/21286 | |
| dc.identifier.vancouvercitation | De Kock SH, Du Preez JC, Kilian SG. Anomalies in the growth kinetics of Saccharomyces cerevisiae strains in aerobic chemostat cultures. J Indust Biotechnol 24: 231-236. 2000; http://hdl.handle.net/11427/21286. | en_ZA |
| dc.language | eng | en_ZA |
| dc.publisher | Springer | en_ZA |
| dc.publisher.institution | University of Cape Town | |
| dc.source | J Indust Biotechnol 24: 231-236 | en_ZA |
| dc.source.uri | http://link.springer.com/journal/10295 | |
| dc.subject.other | Saccharomyces cerevisiae | |
| dc.subject.other | aerobic | |
| dc.subject.other | chemostat | |
| dc.subject.other | growth kinetics | |
| dc.title | Anomalies in the growth kinetics of Saccharomyces cerevisiae strains in aerobic chemostat cultures | en_ZA |
| dc.type | Journal Article | en_ZA |
| uct.type.filetype | Text | |
| uct.type.filetype | Image | |
| uct.type.publication | Research | en_ZA |
| uct.type.resource | Article | en_ZA |