A novel microwell-based analytical technique for studying ferrous iron biooxidation activity
| dc.contributor.author | Meissner, Murray P | |
| dc.contributor.author | Xu, Zhehua | |
| dc.contributor.author | Jones, Gavin C | |
| dc.contributor.author | Minnaar, Susanna H | |
| dc.contributor.author | Harrison, Susan T L | |
| dc.date.accessioned | 2016-08-15T11:32:48Z | |
| dc.date.available | 2016-08-15T11:32:48Z | |
| dc.date.issued | 2014 | |
| dc.date.updated | 2016-08-15T11:31:10Z | |
| dc.description.abstract | The broad range of complexities in bioleaching includes the use of mixed microbial communities with diversity of species and strains with different windows of operating conditions. Empirical approaches to characterise these currently use cumbersome experimental systems; hence the need to develop a high throughput research tool, analogous to the techniques used in high throughput pharmaceutical research. In this study, a microwell research tool was evaluated as a growth and measurement tool for mixed autotrophic bioleaching cultures. The tool was assessed by comparing its performance to conventional shake flask apparatus. Mixed mesophilic cultures of predominantly Leptospirillum ferriphilum and Acidithiobacillus ferrooxidans were used. Growth and ferrous iron oxidation kinetics were quantified and assessed. Microwell plates performed similarly to conventional shake flasks with respect to growth and iron oxidation kinetics. The microwell plate apparatus was also used as a measurement system in combination with a microwell plate reader (measuring absorbance change at 428 nm over time). Progressive colour change of growth experiments correlated to ferrous iron oxidation within a defined operating window. We conclude that, using this measurement as a proxy for trends iron oxidation, the microwell research tool is well-suited for high throughput scoping studies to map operating windows for different cultures, in both an unadapted and adapted context. This was confirmed through an activity test utilising fluoride as an inhibitor. Where absorbance measurements at 428 nm are used to track oxidation progress, the research tool has limitations with respect to pH (<2.0) and total iron concentration (<8.0 g l−1). | en_ZA |
| dc.description.uri | http://www.sciencedirect.com/science/article/pii/S0892687514000132 | |
| dc.identifier | http://dx.doi.org/10.1016/j.mineng.2014.01.012 | |
| dc.identifier.apacitation | Meissner, M. P., Xu, Z., Jones, G. C., Minnaar, S. H., & Harrison, S. T. L. (2014). A novel microwell-based analytical technique for studying ferrous iron biooxidation activity. <i>Minerals Engineering</i>, http://hdl.handle.net/11427/21236 | en_ZA |
| dc.identifier.chicagocitation | Meissner, Murray P, Zhehua Xu, Gavin C Jones, Susanna H Minnaar, and Susan T L Harrison "A novel microwell-based analytical technique for studying ferrous iron biooxidation activity." <i>Minerals Engineering</i> (2014) http://hdl.handle.net/11427/21236 | en_ZA |
| dc.identifier.citation | Meissner, M. P., Xu, Z., Jones, G. C., Minnaar, S. H., & Harrison, S. T. (2014). A novel microwell-based analytical technique for studying ferrous iron biooxidation activity. Minerals Engineering, 60, 8-13. | en_ZA |
| dc.identifier.issn | 0892-6875 | en_ZA |
| dc.identifier.ris | TY - Journal Article AU - Meissner, Murray P AU - Xu, Zhehua AU - Jones, Gavin C AU - Minnaar, Susanna H AU - Harrison, Susan T L AB - The broad range of complexities in bioleaching includes the use of mixed microbial communities with diversity of species and strains with different windows of operating conditions. Empirical approaches to characterise these currently use cumbersome experimental systems; hence the need to develop a high throughput research tool, analogous to the techniques used in high throughput pharmaceutical research. In this study, a microwell research tool was evaluated as a growth and measurement tool for mixed autotrophic bioleaching cultures. The tool was assessed by comparing its performance to conventional shake flask apparatus. Mixed mesophilic cultures of predominantly Leptospirillum ferriphilum and Acidithiobacillus ferrooxidans were used. Growth and ferrous iron oxidation kinetics were quantified and assessed. Microwell plates performed similarly to conventional shake flasks with respect to growth and iron oxidation kinetics. The microwell plate apparatus was also used as a measurement system in combination with a microwell plate reader (measuring absorbance change at 428 nm over time). Progressive colour change of growth experiments correlated to ferrous iron oxidation within a defined operating window. We conclude that, using this measurement as a proxy for trends iron oxidation, the microwell research tool is well-suited for high throughput scoping studies to map operating windows for different cultures, in both an unadapted and adapted context. This was confirmed through an activity test utilising fluoride as an inhibitor. Where absorbance measurements at 428 nm are used to track oxidation progress, the research tool has limitations with respect to pH (<2.0) and total iron concentration (<8.0 g l−1). DA - 2014 DB - OpenUCT DP - University of Cape Town J1 - Minerals Engineering KW - Mesophiles KW - Microwell plate KW - High throughput research LK - https://open.uct.ac.za PB - University of Cape Town PY - 2014 SM - 0892-6875 T1 - A novel microwell-based analytical technique for studying ferrous iron biooxidation activity TI - A novel microwell-based analytical technique for studying ferrous iron biooxidation activity UR - http://hdl.handle.net/11427/21236 ER - | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/21236 | |
| dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0892687514000132 | |
| dc.identifier.vancouvercitation | Meissner MP, Xu Z, Jones GC, Minnaar SH, Harrison STL. A novel microwell-based analytical technique for studying ferrous iron biooxidation activity. Minerals Engineering. 2014; http://hdl.handle.net/11427/21236. | en_ZA |
| dc.language | eng | en_ZA |
| dc.publisher | Elsevier | en_ZA |
| dc.publisher.institution | University of Cape Town | |
| dc.source | Minerals Engineering | en_ZA |
| dc.source.uri | http://www.journals.elsevier.com/minerals-engineering/ | |
| dc.subject | Mesophiles | |
| dc.subject | Microwell plate | |
| dc.subject | High throughput research | |
| dc.subject.other | Bioleaching | |
| dc.title | A novel microwell-based analytical technique for studying ferrous iron biooxidation activity | en_ZA |
| dc.type | Journal Article | en_ZA |
| uct.type.filetype | Text | |
| uct.type.filetype | Image | |
| uct.type.publication | Research | en_ZA |
| uct.type.resource | Article | en_ZA |