Kinetic analysis of biological sulphate reduction using lactate as carbon source and electron donor across a range of sulphate concentrations
| dc.contributor.author | Oyekola, O O | |
| dc.contributor.author | van Hille, R P | |
| dc.contributor.author | Harrison, S T L | |
| dc.date.accessioned | 2016-08-22T13:42:39Z | |
| dc.date.available | 2016-08-22T13:42:39Z | |
| dc.date.issued | 2010 | |
| dc.date.updated | 2016-08-19T13:08:12Z | |
| dc.description.abstract | This study investigated the effect of feed sulphate concentration on the kinetics of anaerobic sulphate reduction by a mixed SRB culture, using lactate as the sole carbon source and electron donor. Chemostat cultures were operated across a range of residence times (0.5–5 d) and feed sulphate concentrations (1.0–10.0 g l−1). Similar phenomena were observed at feed sulphate concentrations of 1.0 and 10.0 g l−1 with the volumetric sulphate reduction rate increasing linearly with increasing volumetric sulphate loading rate. These reactors were characterised by higher specific volumetric sulphate reduction rates with maximum values of 0.24 and 0.20 g h−1 g−1. Contrastingly, the reactors fed with sulphate concentrations of 2.5 and 5.0 g l−1 showed distinctly different trends in which the volumetric sulphate reduction rate passed through a maximum at the dilution rates of 0.014 and 0.021 h−1, respectively, followed by a decline with further increase in sulphate loading rate. The maximum specific volumetric sulphate reduction rates observed were 2–6-fold lower than those observed at 1.0 and 10.0 g l−1 feed sulphate concentrations. Profiles of specific volumetric sulphate reduction rate and biomass concentration suggested a shift in lactate utilisation from oxidation to fermentation at high dilution rates, implying a change in the dominant components of the microbial consortium. The data suggest that population structure was influenced by lactate affinity and dissolved sulphide concentration. The trends observed were attributed to the greater ability of lactate oxidisers to scavenge lactate under limiting concentrations of the substrate and their greater resilience to dissolved sulphide species in comparison to lactate fermenters. | en_ZA |
| dc.identifier | http://dx.doi.org/10.1016/j.ces.2010.05.014 | |
| dc.identifier.apacitation | Oyekola, O. O., van Hille, R. P., & Harrison, S. T. L. (2010). Kinetic analysis of biological sulphate reduction using lactate as carbon source and electron donor across a range of sulphate concentrations. <i>Chemical Engineering Science</i>, http://hdl.handle.net/11427/21449 | en_ZA |
| dc.identifier.chicagocitation | Oyekola, O O, R P van Hille, and S T L Harrison "Kinetic analysis of biological sulphate reduction using lactate as carbon source and electron donor across a range of sulphate concentrations." <i>Chemical Engineering Science</i> (2010) http://hdl.handle.net/11427/21449 | en_ZA |
| dc.identifier.citation | Oyekola, O. O., van Hille, R. P., & Harrison, S. T. (2010). Kinetic analysis of biological sulphate reduction using lactate as carbon source and electron donor: effect of sulphate concentration. Chemical Engineering Science, 65(16), 4771-4781. | en_ZA |
| dc.identifier.issn | 0009-2509 | en_ZA |
| dc.identifier.ris | TY - Journal Article AU - Oyekola, O O AU - van Hille, R P AU - Harrison, S T L AB - This study investigated the effect of feed sulphate concentration on the kinetics of anaerobic sulphate reduction by a mixed SRB culture, using lactate as the sole carbon source and electron donor. Chemostat cultures were operated across a range of residence times (0.5–5 d) and feed sulphate concentrations (1.0–10.0 g l−1). Similar phenomena were observed at feed sulphate concentrations of 1.0 and 10.0 g l−1 with the volumetric sulphate reduction rate increasing linearly with increasing volumetric sulphate loading rate. These reactors were characterised by higher specific volumetric sulphate reduction rates with maximum values of 0.24 and 0.20 g h−1 g−1. Contrastingly, the reactors fed with sulphate concentrations of 2.5 and 5.0 g l−1 showed distinctly different trends in which the volumetric sulphate reduction rate passed through a maximum at the dilution rates of 0.014 and 0.021 h−1, respectively, followed by a decline with further increase in sulphate loading rate. The maximum specific volumetric sulphate reduction rates observed were 2–6-fold lower than those observed at 1.0 and 10.0 g l−1 feed sulphate concentrations. Profiles of specific volumetric sulphate reduction rate and biomass concentration suggested a shift in lactate utilisation from oxidation to fermentation at high dilution rates, implying a change in the dominant components of the microbial consortium. The data suggest that population structure was influenced by lactate affinity and dissolved sulphide concentration. The trends observed were attributed to the greater ability of lactate oxidisers to scavenge lactate under limiting concentrations of the substrate and their greater resilience to dissolved sulphide species in comparison to lactate fermenters. DA - 2010 DB - OpenUCT DP - University of Cape Town J1 - Chemical Engineering Science LK - https://open.uct.ac.za PB - University of Cape Town PY - 2010 SM - 0009-2509 T1 - Kinetic analysis of biological sulphate reduction using lactate as carbon source and electron donor across a range of sulphate concentrations TI - Kinetic analysis of biological sulphate reduction using lactate as carbon source and electron donor across a range of sulphate concentrations UR - http://hdl.handle.net/11427/21449 ER - | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/21449 | |
| dc.identifier.vancouvercitation | Oyekola OO, van Hille RP, Harrison STL. Kinetic analysis of biological sulphate reduction using lactate as carbon source and electron donor across a range of sulphate concentrations. Chemical Engineering Science. 2010; http://hdl.handle.net/11427/21449. | en_ZA |
| dc.language | eng | en_ZA |
| dc.publisher | Elsevier | en_ZA |
| dc.publisher.institution | University of Cape Town | |
| dc.rights | Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_ZA |
| dc.source | Chemical Engineering Science | en_ZA |
| dc.source.uri | http://www.journals.elsevier.com/chemical-engineering-science/ | |
| dc.subject.other | Acid mine drainage | |
| dc.subject.other | Bioprocessing | |
| dc.subject.other | Bioreactors | |
| dc.subject.other | Environment | |
| dc.subject.other | Kinetics | |
| dc.title | Kinetic analysis of biological sulphate reduction using lactate as carbon source and electron donor across a range of sulphate concentrations | en_ZA |
| dc.type | Journal Article | en_ZA |
| uct.type.filetype | Text | |
| uct.type.filetype | Image | |
| uct.type.publication | Research | en_ZA |
| uct.type.resource | Article | en_ZA |