The role of Surfacant Protein D in the control of human helminth infections
| dc.contributor.advisor | Horsnell, William | |
| dc.contributor.author | Baker, Zoe | |
| dc.date.accessioned | 2021-01-20T08:41:50Z | |
| dc.date.available | 2021-01-20T08:41:50Z | |
| dc.date.issued | 2020 | |
| dc.date.updated | 2020-12-23T09:21:37Z | |
| dc.description.abstract | Lung produced surfactant protein D (SP-D) is essential for both homeostasis and as an innate immune opsonin. In the project presented here, we aimed to translate data recently published by our group, which demonstrated that SP-D contributes to protection against murine parasitic nematode infections, to human work. In the first part of this study, we determined whether individuals exposed to helminths have altered serum SP-D in comparison to unexposed individuals, through analysis (ELISA and Western Blot) of bio banked samples in 2 clinical cohorts from South Africa. Secondly, we aimed to identify if SP-D influences the magnitude of anti-nematode responses in human immune cells (type 2 innate lymphoid cells, monocytes and macrophages) through in vitro cell work and flow cytometry. Our findings indicated an association between serum SP-D and exposure to helminths that have a lung migration stage as part of their life cycle (Ascaris spp and Toxocara spp). Furthermore, in vitro analysis demonstrated that human immune cells primed with SP-D might have an altered response to helminth antigen. These findings point toward the need for further investigation into the novel role of SP-D in the control of human helminth infections in the context of immune physiology, as a biomarker and eventually treatment option. | |
| dc.identifier.apacitation | Baker, Z. (2020). <i>The role of Surfacant Protein D in the control of human helminth infections</i>. (). ,Faculty of Health Sciences ,Division of Immunology. Retrieved from http://hdl.handle.net/11427/32585 | en_ZA |
| dc.identifier.chicagocitation | Baker, Zoe. <i>"The role of Surfacant Protein D in the control of human helminth infections."</i> ., ,Faculty of Health Sciences ,Division of Immunology, 2020. http://hdl.handle.net/11427/32585 | en_ZA |
| dc.identifier.citation | Baker, Z. 2020. The role of Surfacant Protein D in the control of human helminth infections. . ,Faculty of Health Sciences ,Division of Immunology. http://hdl.handle.net/11427/32585 | en_ZA |
| dc.identifier.ris | TY - Master Thesis AU - Baker, Zoe AB - Lung produced surfactant protein D (SP-D) is essential for both homeostasis and as an innate immune opsonin. In the project presented here, we aimed to translate data recently published by our group, which demonstrated that SP-D contributes to protection against murine parasitic nematode infections, to human work. In the first part of this study, we determined whether individuals exposed to helminths have altered serum SP-D in comparison to unexposed individuals, through analysis (ELISA and Western Blot) of bio banked samples in 2 clinical cohorts from South Africa. Secondly, we aimed to identify if SP-D influences the magnitude of anti-nematode responses in human immune cells (type 2 innate lymphoid cells, monocytes and macrophages) through in vitro cell work and flow cytometry. Our findings indicated an association between serum SP-D and exposure to helminths that have a lung migration stage as part of their life cycle (Ascaris spp and Toxocara spp). Furthermore, in vitro analysis demonstrated that human immune cells primed with SP-D might have an altered response to helminth antigen. These findings point toward the need for further investigation into the novel role of SP-D in the control of human helminth infections in the context of immune physiology, as a biomarker and eventually treatment option. DA - 2020_ DB - OpenUCT DP - University of Cape Town KW - Clinical Science and Immunology LK - https://open.uct.ac.za PY - 2020 T1 - The role of Surfacant Protein D in the control of human helminth infections TI - The role of Surfacant Protein D in the control of human helminth infections UR - http://hdl.handle.net/11427/32585 ER - | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/32585 | |
| dc.identifier.vancouvercitation | Baker Z. The role of Surfacant Protein D in the control of human helminth infections. []. ,Faculty of Health Sciences ,Division of Immunology, 2020 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/32585 | en_ZA |
| dc.language.rfc3066 | eng | |
| dc.publisher.department | Division of Immunology | |
| dc.publisher.faculty | Faculty of Health Sciences | |
| dc.subject | Clinical Science and Immunology | |
| dc.title | The role of Surfacant Protein D in the control of human helminth infections | |
| dc.type | Master Thesis | |
| dc.type.qualificationlevel | Masters | |
| dc.type.qualificationlevel | MSc |