Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus

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dc.contributor.advisorKirby, Ralphen_ZA
dc.contributor.authorBotha, Christine Leoneen_ZA
dc.date.accessioned2015-11-30T08:28:32Z
dc.date.available2015-11-30T08:28:32Z
dc.date.issued1981en_ZA
dc.descriptionBibliography: leaves 89-96.en_ZA
dc.description.abstractThe Actinomycete organism, Streptomyces alboflavus, produces the antibiotic oxytetracycline. Treatment with curing agents resulted in S. alboflavus losing the ability to produce oxytetracycline. However, the loss of oxytetracyc1ine production was reversible, and non-producing colonies regained the ability to produce oxytetracycline when the curing agent was removed. Therefore the loss of oxytetracyc1ine production was not due to the irreversible loss of genetic material specifying oxytetracycline production, but was possibly due to genetic instability. Two extrachromosomal DNA species were isolated from S. alboflavus, (SAP1 and SAP2). SAP1 was approximately 8 - 10 x 10â ¶ da1tons, and appeared to be linear and heterogenous. SAP2 was 20 - 25 x 10â ¶ daltons and appeared to be covalently closed circular. The functions of SAP1 and SAP2 are unknown, although transformation experiments with SAP1 suggested that it may play a role in the production of an antibiotic-like substance, possibly by acting as a promotor of the genes coding for the antibiotic-like substance.en_ZA
dc.identifier.apacitationBotha, C. L. (1981). <i>Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/15447en_ZA
dc.identifier.chicagocitationBotha, Christine Leone. <i>"Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1981. http://hdl.handle.net/11427/15447en_ZA
dc.identifier.citationBotha, C. 1981. Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Botha, Christine Leone AB - The Actinomycete organism, Streptomyces alboflavus, produces the antibiotic oxytetracycline. Treatment with curing agents resulted in S. alboflavus losing the ability to produce oxytetracycline. However, the loss of oxytetracyc1ine production was reversible, and non-producing colonies regained the ability to produce oxytetracycline when the curing agent was removed. Therefore the loss of oxytetracyc1ine production was not due to the irreversible loss of genetic material specifying oxytetracycline production, but was possibly due to genetic instability. Two extrachromosomal DNA species were isolated from S. alboflavus, (SAP1 and SAP2). SAP1 was approximately 8 - 10 x 10â ¶ da1tons, and appeared to be linear and heterogenous. SAP2 was 20 - 25 x 10â ¶ daltons and appeared to be covalently closed circular. The functions of SAP1 and SAP2 are unknown, although transformation experiments with SAP1 suggested that it may play a role in the production of an antibiotic-like substance, possibly by acting as a promotor of the genes coding for the antibiotic-like substance. DA - 1981 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1981 T1 - Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus TI - Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus UR - http://hdl.handle.net/11427/15447 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/15447
dc.identifier.vancouvercitationBotha CL. Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1981 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/15447en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMicrobiologyen_ZA
dc.titleGeneric studies on the oxytetracycline producing organism, Streptomyces alboflavusen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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