An investigation into the synergistic action of chemotherapy and photodynamic therapy in resistant skin cancers

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dc.contributor.advisorDavids, Lester Men_ZA
dc.contributor.authorNsole Biteghe, Fleury Augustinen_ZA
dc.date.accessioned2015-09-30T13:43:33Z
dc.date.available2015-09-30T13:43:33Z
dc.date.issued2015en_ZA
dc.description.abstractMelanoma is a form of skin cancer, arising from epidermal cells of the melanocyte lineage, which undergo a series of transformations and genetic alterations that may give rise to both pigmented and unpigmented melanoma. Melanoma represents 4% of all skin cancers but due to its aggressive nature, it accounts for 80% of death among skin cancer patients. South Africa has a melanoma incidence rate that is second worldwide to only Australia. In melanoma; non-metastatic primary tumours are treated by surgical resection. However, metastatic melanoma is highly resistant to conventional radio and chemotherapy, thus reducing the median life of patient's diagnosis with the metastatic form to about 7-9months. Given the implications of the pigment in failure of chemotherapy, two human metastatic pigmented and unpigmented melanoma cell lines were used to investigate the mechanisms underlying chemo-resistance. During the course of this study, the first aim was to determine the concentration of the chemotherapeutic drug dacarbazine (DTIC) causing fifty percent decrease in melanoma cell viability (LD50), then to investigate the possible synergism of hypericin activated-photodynamic therapy in reducing (HYP-PDT) melanoma cell viability, when combined with chemotherapy. In addition we wanted to assess the morphology and the clonogenic capacity of the melanoma cells, after the different treatments and further investigate the ATP-binding cassette (ABC) transporters (ABCB5/1 & ABCG2) expression profile, before and after chemotherapeutic (DTIC) and combination therapy (DTIC+HYP-PDT) treatments.en_ZA
dc.identifier.apacitationNsole Biteghe, F. A. (2015). <i>An investigation into the synergistic action of chemotherapy and photodynamic therapy in resistant skin cancers</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Department of Human Biology. Retrieved from http://hdl.handle.net/11427/14133en_ZA
dc.identifier.chicagocitationNsole Biteghe, Fleury Augustin. <i>"An investigation into the synergistic action of chemotherapy and photodynamic therapy in resistant skin cancers."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Department of Human Biology, 2015. http://hdl.handle.net/11427/14133en_ZA
dc.identifier.citationNsole Biteghe, F. 2015. An investigation into the synergistic action of chemotherapy and photodynamic therapy in resistant skin cancers. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Nsole Biteghe, Fleury Augustin AB - Melanoma is a form of skin cancer, arising from epidermal cells of the melanocyte lineage, which undergo a series of transformations and genetic alterations that may give rise to both pigmented and unpigmented melanoma. Melanoma represents 4% of all skin cancers but due to its aggressive nature, it accounts for 80% of death among skin cancer patients. South Africa has a melanoma incidence rate that is second worldwide to only Australia. In melanoma; non-metastatic primary tumours are treated by surgical resection. However, metastatic melanoma is highly resistant to conventional radio and chemotherapy, thus reducing the median life of patient's diagnosis with the metastatic form to about 7-9months. Given the implications of the pigment in failure of chemotherapy, two human metastatic pigmented and unpigmented melanoma cell lines were used to investigate the mechanisms underlying chemo-resistance. During the course of this study, the first aim was to determine the concentration of the chemotherapeutic drug dacarbazine (DTIC) causing fifty percent decrease in melanoma cell viability (LD50), then to investigate the possible synergism of hypericin activated-photodynamic therapy in reducing (HYP-PDT) melanoma cell viability, when combined with chemotherapy. In addition we wanted to assess the morphology and the clonogenic capacity of the melanoma cells, after the different treatments and further investigate the ATP-binding cassette (ABC) transporters (ABCB5/1 & ABCG2) expression profile, before and after chemotherapeutic (DTIC) and combination therapy (DTIC+HYP-PDT) treatments. DA - 2015 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2015 T1 - An investigation into the synergistic action of chemotherapy and photodynamic therapy in resistant skin cancers TI - An investigation into the synergistic action of chemotherapy and photodynamic therapy in resistant skin cancers UR - http://hdl.handle.net/11427/14133 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/14133
dc.identifier.vancouvercitationNsole Biteghe FA. An investigation into the synergistic action of chemotherapy and photodynamic therapy in resistant skin cancers. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Department of Human Biology, 2015 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/14133en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Human Biologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleAn investigation into the synergistic action of chemotherapy and photodynamic therapy in resistant skin cancersen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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