Fermentation studies on Clostridium acetabutylicum

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dc.contributor.advisorWoods, David Ren_ZA
dc.contributor.advisorJones, David Ten_ZA
dc.contributor.authorVan der Westhuizen, Andréen_ZA
dc.date.accessioned2016-10-10T14:57:01Z
dc.date.available2016-10-10T14:57:01Z
dc.date.issued1982en_ZA
dc.descriptionBibliography: pages 147-162.en_ZA
dc.description.abstractThe initial aim of this work was to develop a laboratory system for the study of the ABE fermentation under laboratory conditions. The development of defined and simple laboratory inoculation and build-up procedures for the ABE process was investigated. A defined spore preparation in distilled water gave solvent yields comparable to the yields obtained in the commercial ABE process. A laboratory inoculation procedure was developed which avoided the lengthy culture build-up procedures presently utilised. An investigation into solvent production by Clostridium acetobutylicum in clostridial basal medium (CBM) , reinforced clostridial medium (RCM) , Leung and Robson media was undertaken with the aim of developing a partially defined laboratory medium which produced solvent yields comparable to the molasses fermentation medium (MFM). The solvent yields obtained in the partially defined laboratory media were substantially lower than those obtained in MFM. It became apparent that the initial aim of trying to identify and manipulate a few key factors to give better solvent yields would not be easily attained. Both the solvent levels and the overall pattern of cell development were markedly different in the various laboratory systems. In view of these differences, a more detailed investigation of the growth patterns, morphological and physiological changes were undertaken.en_ZA
dc.identifier.apacitationVan der Westhuizen, A. (1982). <i>Fermentation studies on Clostridium acetabutylicum</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/22111en_ZA
dc.identifier.chicagocitationVan der Westhuizen, André. <i>"Fermentation studies on Clostridium acetabutylicum."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1982. http://hdl.handle.net/11427/22111en_ZA
dc.identifier.citationVan der Westhuizen, A. 1982. Fermentation studies on Clostridium acetabutylicum. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Van der Westhuizen, André AB - The initial aim of this work was to develop a laboratory system for the study of the ABE fermentation under laboratory conditions. The development of defined and simple laboratory inoculation and build-up procedures for the ABE process was investigated. A defined spore preparation in distilled water gave solvent yields comparable to the yields obtained in the commercial ABE process. A laboratory inoculation procedure was developed which avoided the lengthy culture build-up procedures presently utilised. An investigation into solvent production by Clostridium acetobutylicum in clostridial basal medium (CBM) , reinforced clostridial medium (RCM) , Leung and Robson media was undertaken with the aim of developing a partially defined laboratory medium which produced solvent yields comparable to the molasses fermentation medium (MFM). The solvent yields obtained in the partially defined laboratory media were substantially lower than those obtained in MFM. It became apparent that the initial aim of trying to identify and manipulate a few key factors to give better solvent yields would not be easily attained. Both the solvent levels and the overall pattern of cell development were markedly different in the various laboratory systems. In view of these differences, a more detailed investigation of the growth patterns, morphological and physiological changes were undertaken. DA - 1982 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1982 T1 - Fermentation studies on Clostridium acetabutylicum TI - Fermentation studies on Clostridium acetabutylicum UR - http://hdl.handle.net/11427/22111 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/22111
dc.identifier.vancouvercitationVan der Westhuizen A. Fermentation studies on Clostridium acetabutylicum. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1982 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/22111en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMicrobiologyen_ZA
dc.titleFermentation studies on Clostridium acetabutylicumen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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