Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements

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dc.contributor.authorRooyen, Jason van
dc.contributor.authorBelrhali, Hassan
dc.contributor.authorAbratt, Valarie
dc.contributor.authorSewel, Trevor B
dc.date.accessioned2016-07-27T12:13:25Z
dc.date.available2016-07-27T12:13:25Z
dc.date.issued2011
dc.date.updated2016-07-27T12:11:56Z
dc.description.abstractThis work details the intentional modifications that led to the first structure of a type III glutamine synthetase enzyme (GSIII). This approach followed the serendipitous discovery of digestion caused by an extracellular protease from a contaminating bacterium, Pseudomonas fluorescens. The protease only cleaves the GSIII protein at a single site, leaving the oligomer intact but allowing the protein to crystallize in a different space group. This transition from space group P1 to space group C2221 is accompanied by improved growth characteristics, more reproducible diffraction and enhanced mechanical stability. The crystallographic analyses presented here provide the structural basis of the altered molecular packing in the full-length and digested crystal forms and suggest modifications for future structural studies.en_ZA
dc.identifierhttp://dx.doi.org/10.1107/S1744309110053893
dc.identifier.apacitationRooyen, J. v., Belrhali, H., Abratt, V., & Sewel, T. B. (2011). Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements. <i>Acta Crystallographica Section F Structural Biology and Crystallization Communications</i>, http://hdl.handle.net/11427/20889en_ZA
dc.identifier.chicagocitationRooyen, Jason van, Hassan Belrhali, Valarie Abratt, and Trevor B Sewel "Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements." <i>Acta Crystallographica Section F Structural Biology and Crystallization Communications</i> (2011) http://hdl.handle.net/11427/20889en_ZA
dc.identifier.citationvan Rooyen, J., Belrhali, H., Abratt, V., & Sewell, B. T. (2011). Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 67(3), 358-363.en_ZA
dc.identifier.issn1744-309en_ZA
dc.identifier.ris TY - Journal Article AU - Rooyen, Jason van AU - Belrhali, Hassan AU - Abratt, Valarie AU - Sewel, Trevor B AB - This work details the intentional modifications that led to the first structure of a type III glutamine synthetase enzyme (GSIII). This approach followed the serendipitous discovery of digestion caused by an extracellular protease from a contaminating bacterium, Pseudomonas fluorescens. The protease only cleaves the GSIII protein at a single site, leaving the oligomer intact but allowing the protein to crystallize in a different space group. This transition from space group P1 to space group C2221 is accompanied by improved growth characteristics, more reproducible diffraction and enhanced mechanical stability. The crystallographic analyses presented here provide the structural basis of the altered molecular packing in the full-length and digested crystal forms and suggest modifications for future structural studies. DA - 2011 DB - OpenUCT DP - University of Cape Town J1 - Acta Crystallographica Section F Structural Biology and Crystallization Communications LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 SM - 1744-309 T1 - Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements TI - Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements UR - http://hdl.handle.net/11427/20889 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20889
dc.identifier.urihttp://journals.iucr.org/f/issues/2011/03/00/pu5321/pu5321.pdf
dc.identifier.vancouvercitationRooyen Jv, Belrhali H, Abratt V, Sewel TB. Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements. Acta Crystallographica Section F Structural Biology and Crystallization Communications. 2011; http://hdl.handle.net/11427/20889.en_ZA
dc.languageengen_ZA
dc.publisherInternational Union of Crystallographyen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceActa Crystallographica Section F Structural Biology and Crystallization Communicationsen_ZA
dc.source.urihttp://journals.iucr.org/f/index.html
dc.subject.otherProteolysis
dc.subject.othertype III glutamine synthetase
dc.subject.otherBacteroides fragilis
dc.subject.othercrystal-packing
dc.titleProteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangementsen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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