The kinetics of endosome processing

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dc.contributor.advisorThilo, Lutzen_ZA
dc.contributor.authorLegalatladi, Seetselaen_ZA
dc.date.accessioned2018-01-29T07:13:44Z
dc.date.available2018-01-29T07:13:44Z
dc.date.issued1995en_ZA
dc.description.abstractThe present thesis looks at the behaviour of internalised cell surface-derived membrane marker in comparison with the behaviour of endocytosed HRP (horse-radish peroxidase) as a fluid-phase contents marker. The pooling and/or segregation in the endosome was measured by determining co-localization with HRP. Colocalization of the two markers in the endosome is studied by using the ability of HRP to catalyse the crosslinking of membrane marker in endosomes with DAB (3,3'-diaminobenzidine), rendering the membrane marker detergent insoluble. To study the kinetic behaviour of membrane marker, radioactive galactose was covalently bound to cell-surface glycoconjugates on mouse macrophage-cells, P388D₁, as catalysed by galactosyltransferase. This provided a general membrane marker. After endocytosis-derived redistribution of membrane marker between the cell surface and endosomal membrane, a steady state was established with about 16% of the label on internal membranes. The bulk of the label on the cell surface was removable by subsequent treatment with β-galactosidase.en_ZA
dc.identifier.apacitationLegalatladi, S. (1995). <i>The kinetics of endosome processing</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Biomedical Engineering. Retrieved from http://hdl.handle.net/11427/27047en_ZA
dc.identifier.chicagocitationLegalatladi, Seetsela. <i>"The kinetics of endosome processing."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Biomedical Engineering, 1995. http://hdl.handle.net/11427/27047en_ZA
dc.identifier.citationLegalatladi, S. 1995. The kinetics of endosome processing. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Legalatladi, Seetsela AB - The present thesis looks at the behaviour of internalised cell surface-derived membrane marker in comparison with the behaviour of endocytosed HRP (horse-radish peroxidase) as a fluid-phase contents marker. The pooling and/or segregation in the endosome was measured by determining co-localization with HRP. Colocalization of the two markers in the endosome is studied by using the ability of HRP to catalyse the crosslinking of membrane marker in endosomes with DAB (3,3'-diaminobenzidine), rendering the membrane marker detergent insoluble. To study the kinetic behaviour of membrane marker, radioactive galactose was covalently bound to cell-surface glycoconjugates on mouse macrophage-cells, P388D₁, as catalysed by galactosyltransferase. This provided a general membrane marker. After endocytosis-derived redistribution of membrane marker between the cell surface and endosomal membrane, a steady state was established with about 16% of the label on internal membranes. The bulk of the label on the cell surface was removable by subsequent treatment with β-galactosidase. DA - 1995 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1995 T1 - The kinetics of endosome processing TI - The kinetics of endosome processing UR - http://hdl.handle.net/11427/27047 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/27047
dc.identifier.vancouvercitationLegalatladi S. The kinetics of endosome processing. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Biomedical Engineering, 1995 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/27047en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Biomedical Engineeringen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBiomedical Engineeringen_ZA
dc.subject.otherEndocytosisen_ZA
dc.subject.otherEndosomesen_ZA
dc.subject.otherBiological transporten_ZA
dc.titleThe kinetics of endosome processingen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMSc (Med)en_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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