An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins
| dc.contributor.author | Windram, Oliver P | |
| dc.contributor.author | Weber, Brandon | |
| dc.contributor.author | Jaffer, Mohamed A | |
| dc.contributor.author | Rybicki, Edward P | |
| dc.contributor.author | Shepherd, Dionne N | |
| dc.contributor.author | Varsani, Arvind | |
| dc.date.accessioned | 2016-07-13T10:54:03Z | |
| dc.date.available | 2016-07-13T10:54:03Z | |
| dc.date.issued | 2008 | |
| dc.date.updated | 2016-07-12T17:53:03Z | |
| dc.description.abstract | Development of vaccine strategies against human papillomavirus (HPV), which causes cervical cancer, is a priority. We investigated the use of virus-like particles (VLPs) of the most prevalent type, HPV-16, as carriers of foreign proteins. Green fluorescent protein (GFP) was fused to the N or C terminus of both L1 and L2, with L2 chimeras being co-expressed with native L1. Purified chimaeric VLPs were comparable in size (*55 nm) to native HPV VLPs. Conformation-specific monoclonal antibodies (Mabs) bound to the VLPs, thereby indicating that they possibly retain their antigenicity. In addition, all of the VLPs encapsidated DNA in the range of 6–8 kb. | en_ZA |
| dc.identifier | http://dx.doi.org/10.1007/s00705-007-0025-2 | |
| dc.identifier.apacitation | Windram, O. P., Weber, B., Jaffer, M. A., Rybicki, E. P., Shepherd, D. N., & Varsani, A. (2008). An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins. <i>Archives of Virology</i>, http://hdl.handle.net/11427/20337 | en_ZA |
| dc.identifier.chicagocitation | Windram, Oliver P, Brandon Weber, Mohamed A Jaffer, Edward P Rybicki, Dionne N Shepherd, and Arvind Varsani "An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins." <i>Archives of Virology</i> (2008) http://hdl.handle.net/11427/20337 | en_ZA |
| dc.identifier.citation | Windram, O. P., Weber, B., Jaffer, M. A., Rybicki, E. P., Shepherd, D. N., & Varsani, A. (2008). An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N-and C-terminal fusion of GFP to the L1 and L2 capsid proteins. Archives of virology, 153(3), 585-589. | en_ZA |
| dc.identifier.issn | 0304-8608 | en_ZA |
| dc.identifier.ris | TY - Journal Article AU - Windram, Oliver P AU - Weber, Brandon AU - Jaffer, Mohamed A AU - Rybicki, Edward P AU - Shepherd, Dionne N AU - Varsani, Arvind AB - Development of vaccine strategies against human papillomavirus (HPV), which causes cervical cancer, is a priority. We investigated the use of virus-like particles (VLPs) of the most prevalent type, HPV-16, as carriers of foreign proteins. Green fluorescent protein (GFP) was fused to the N or C terminus of both L1 and L2, with L2 chimeras being co-expressed with native L1. Purified chimaeric VLPs were comparable in size (*55 nm) to native HPV VLPs. Conformation-specific monoclonal antibodies (Mabs) bound to the VLPs, thereby indicating that they possibly retain their antigenicity. In addition, all of the VLPs encapsidated DNA in the range of 6–8 kb. DA - 2008 DB - OpenUCT DP - University of Cape Town J1 - Archives of Virology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 SM - 0304-8608 T1 - An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins TI - An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins UR - http://hdl.handle.net/11427/20337 ER - | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/20337 | |
| dc.identifier.vancouvercitation | Windram OP, Weber B, Jaffer MA, Rybicki EP, Shepherd DN, Varsani A. An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins. Archives of Virology. 2008; http://hdl.handle.net/11427/20337. | en_ZA |
| dc.language | eng | en_ZA |
| dc.publisher | Springer Verlag (Germany) | en_ZA |
| dc.publisher.institution | University of Cape Town | |
| dc.source | Archives of Virology | en_ZA |
| dc.source.uri | http://link.springer.com/journal/705 | |
| dc.title | An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins | en_ZA |
| dc.type | Journal Article | en_ZA |
| uct.type.filetype | Text | |
| uct.type.filetype | Image | |
| uct.type.publication | Research | en_ZA |
| uct.type.resource | Article | en_ZA |